(lab) ALP Flashcards
(20 cards)
catalyzes the hydrolysis of various phosphomonoesters with the subsequent production of alcohol
alkaline phosphatase
optimal ph
9-10
heat stability: __C for __ mins
56C, 10mins
if after heating the remaining activity is <20%, the source of ALP elevation is the __
bone
if after heating the remaining activity is >20%, the source of ALP elevation is the __
liver
phenylalanine inhibits __ (75%) and __ (80%) ALP
intestinal, placental
most heat stable abnormal fraction associated with neoplasms
regan isoenzyme
regan isoenzyme is inhibited by
phenylalanine
variant of regan; inihibited by L-leucine
nagao
substrate of bodansky method
B-glycerophosphate / Na-glycerophosphate
substrate of king-armstrong / gutman-gutman method
phenylphosphate / disodium phenylphosphate
continuous monitoring technique of ALP activity based on the molar absorptivity of p-nitrophenol
bowers and mccomb
possible sources of error
hemolysis
delayed anlysis
diet (may elevate the level in group B and O who are secretors)
high fat meal
reference range
30-90 u/L
reagent: activator
magnesium chloride
reagent: substrate
p-nitrophenyl phosphate
ratio of working rgt (r1:r2)
4:1
reagent: buffer
diethanolamine buffer
what activator did we used in the experiment
magnesium chloride
t/f: the working reagent used is readily available
f
