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Flashcards in lab animal past q Deck (151)
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1

This gland is not a specific pheromone producer gland

Rabbit’s face gland

2

specific pheromone producer gland

Gerbil’s umbilical gland
Dog’s cicumanal gland
Hamster’s hip gland

3

Which is not a method for genetic quality control of lab animals?

Back gross breeding

4

methods for genetic quality control of lab animals?

Skin transplantation
SSLP (micro-satellite)
Biomedical markers

5

Drawback of preference tests:

Animals choose in the base of short term conditions

6

Which measurement is used to express energy requirement of laboratory animals?

Metabolic body weight

7

Sentinels are

Animals used to evaluate a group’s health status

8

Which method wouldn't you use to get 1mL of blood from a rat?

Cardiac puncture

9

You cannot buy this type of diet from a commercial supplier

Chemically defined diet

10

you can buy this type of diet from a commercial supplier

commercial pellet
liquid diet
gel

11

Not α2-agonist

Tolmetine

12

α2-agonist

xilazine
detomidine
romofidine

13

Not inherited disease of rat

Hypo-acidosis

14

inherited disease of rat:

deaf
diabetes
obesity

15

The basic method of rodent phenotyping is to look for

Chromosoma test

16

parts of refinement of animal experimentation

Genetic homogeneity
Health status
Proper defined diet

17

Which animals are covered by 63/2010 EEC directive

Vertebrates and Cephalipodes

18

GLP not concerned about conditions under which laboratory

The ones being built

19

Which species can die after oral penicillin treatment

Guinea pig

20

One of them is an important mechanism of nutrigenomics

Changing the signalling pathway

21

Acute toxicity test has an invasiveness category of __ pain

Severe

22

Maintenance energy requirements of laboratory animals

0.45MJ DE x W0.75

23

This disease is not a zoonosis

Ringtail
Sendai virus disease

24

this disease is zoonosis

Lymphatic choriomeningitis of mice
Salmonellosis
Listeriosis
Hantaan virus disease

25

The recommended nutrient allowances

Ensure the reproduction and longevity of the animals

26

Cannot be stage of mouse genital and urinary syndrome

Ulceration

27

Can be stage of mouse genital and urinary syndrome

Bacteraemia
Inflammation
Mating overload

28

Quarantine period not determined by

Number of animals

29

Quarantine period determined by

health status of the animals
species
purpose of use

30

Which species has a tied skin, so cannot grab it without causing pain to the animal?

Guinea pig

31

Not physical euthanasia method

Maceration

32

physical euthanasia method

T61
Cervical dislocation
Decapitation

33

Which species is not a member of Rodentia genus

Marmoset

34

Which species is a member of Rodentia genus

Golden hamster
Gerbil
Guinea pig

35

OECD do not deal with questions about

Validation of in vitro methods

36

Gerbil is a good model for

Epilepsy aorta

37

animal cannot be examined with ultrasound

Golden hamster

38

Not true for chronic mycoplasmosis of mice

Zoonosis

39

true for chronic mycoplasmosis of mice

Agent is Mycoplasma pulmonis
12-18 day old puppies are most disposed
Can appear with Sendai virus

40

The closest species to Syrian hamster in taxonomy is

Gerbil

41

Good species to use for auditory research

Chinchilla

42

Breeding technique which is not used to maintain an out-bred stock

Brother-sister mating

43

Breeding technique which is used to maintain an out-bred stock:

Random breeding
MAI
cross-breeding

44

A contaminated breeding colony (line) should be

Euthanized
Put under negative pressure cabin
Treated by antibiotics
Individually marked

45

In reproductive toxicology, not acceptable treatment

Used only for parents before mating
Between implantation and foetogenesis
Between foetogenesis and parturition From parent before mating till separation from dam

46

It can be used for intra-dermal injection

Mice ear

47

Pasteurella pneumotropica does not attack

Brain

48

Pasteurella pneumotropica does attack

Uterus
Prostate
Lung

49

Environmental enrichment should not be done

Just for human fun

50

Environmental enrichment should be

For the benefit of the animals
With the sense of animals
With some unpredictability

51

How would you restrain a gerbil

Hand around the chest

52

Who will decide on institute level if an animal experiment was necessary or not

Ethical committee (Animal Welfare Body)

53

This female embryo in a rat will achieve breeding maturity (M=Month

1 M

54

The basic method of rodent sexing is to look fo

Ano-genital distance

55

The recommended nutrient allowances

Ensure the optimum functioning of the animals

56

Maintenance energy requirements of laboratory animals and for extrapolation of dosage use

W 0.75

57

The main body of Laboratory Animal Science of the World

CALAS (the Canadian association for laboratory animal science)

58

The closest species to Syrian hamster in taxonomy is

Gerbil

59

What ‘Environmental Enrichment’ means in laboratory

To give bedding material or toys to experimental animal if possible

60

Feeding rancid feed and/or vitamin E deficient feed may cause

Dermatitis

61

Zebradanio is

Omnivore

62

It is an alternative use of animals in experiments

Database search and meta-analysis

63

Sub-cutaneous injury has a severity category of __ pain

Minor

64

Which 3 words called the 3 R s by Russel and Burch

Reduction, Replacement, Refinement

65

It is not true about euthanasia

Can be used to get large amount of blood out of an animal

66

What does ‘knock out animal’ mean

Transgenic animal

67

Which method would you use to get a drop of blood from mice?

Retro-orbital puncture

68

Which name indicates an outbred stock?

.lr:NMRI

69

You cannot use this method for euthanasia on rat

Ketamine

70

You can use this method for euthanasia on rat

Decapitation
Cervical dislocation with weight limit
CO2 inhalation

71

What is the best equipment to maintain a transgenic colony

Base colony in different countries

72

Which species has only provoked (stimulated) ovulation

Ferret

73

What does the expression ‘inbred strain’ mean

Brother-sister mating through at least 20 generations

74

Which species is the best in atherosclerosis research

WHHL-rabbit
Notes – Gerbil

75

You can find a ‘sacculus rotundus or ampulla ilei’ in the

Rabbit

76

This gland is a specific pheromone producer gland

Hamster’s lateral gland

77

Which species has a gallbladder

Golden hamster

78

What does SPF mean

Specific Pathogen Free

79

How would you restrain a rat for per-oral dosing

By grabbing the skin on back and neck

80

Which sentence is not true about ‘Environmental Enrichment’

Device in case that animal can control

81

true about ‘Environmental Enrichment’

All possibilities should be done to improve animal’s well-being what the experiment allows
Always should be evaluated on cross-benefit base
A nest material is an example for that

82

Gnotobiotic (=Axenic) animals

Germ free

83

What does rederivation mean?

Production of germ-free strain by embryo transfer

84

In the practice, it is not possible to produce germ-free colony from this species

Duck

85

Which would be the best feeding regime in an experiment

Ad libitum

86

What is well-being

State of physiological and psychological harmony of the animal with its internal and external environment

87

Which vein can be used for IV injection on ferret

Jugular vein

88

How would you restrain a rabbit

By grabbing as much skin as possible on back

89

You cannot use this method for euthanasia on new-born, nest-living animals

CO2 inhalation

90

Which method would you use you get 10mL of blood from an adult rat?

Preparation of abdominal aorta

91

Which mouse strain is sensitive to Pneumocystis carini

Swiss

92

Which is not necessary before transport of rodent?

Fasting

93

necessary before transport of rodent

Clinical examination
Ear printing
Acclimatisation

94

When this phenomenon disappears, the narcosis ends with death

Cornea reflex

95

It is not true about euthanasia

Should be cheap

96

true about euthanasia

Should achieve rapid unconciseness and death
Should be safe for user
Should be simple to administer

97

not true about the co-isogenic strains

It can only be maintained in a homozygous form

98

true about the co-isogenic strains

The new gene is the product of natural maturation
The name contains the strain/sub-strain and the symbol of the mutant gene
The co-isogenic strain only differs by only one gene from the original one

99

Dog is not a good model for

Cardiovascular atherosclerosis

100

Dog is a good model for

Tumour-cancer research
Leptospirosis
Epilepsy

101

Mini-pig has a live weight of

20-45 kg

102

Experimental design cannot be

Cross-breeding

103

Experimental design can be

Cross-over design
Latin square design
Factorial design

104

The basal circadian rhythm is

Dependent from the gen expression of a protein

105

Melatonin is produced by

Pineal gland

106

This species mostly used in viral respiratory research

Ferrets

107

A chronic stress can cause this type of behaviour

Apathy / Aggression

108

Well-being cannot be measured by (more than one correct answer)

Clinical assessment
Physiological parameters
Grimace score
Reproductive state

109

Which environment is good for animals?

Classical music

110

Which tissue is the least sensitive to suffering?

Brain tissue

111

The naked mole rat has an extra=ordinary long life-span because of

Special hyaluronan which prevent cancer

112

The co-efficient on inbreeding (F

Means the fraction of the genes that get fixed in a homozygous state

113

It is inherited disease of some mouse strains

Deafness

114

Which is a member of Pox viridae

Ectomelia virus

115

Ringtail is caused by

Too low relative humidity

116

Not part of refinement of animal experimentation

Hair colour
Alternative use of animals

117

part of refinement of animal experimentation

Health status
Analgesia
Environmental enrichment

118

The hearing range of rodents

0.5 / 1 - 100 / 120 kHz

119

Which species has 1 pair teats

Guinea pig

120

Which species has a ‘peg’ (double incisors) teeth

Rabbit

121

The two important words for animals concerning the pain feeling (Weiss-trail)

Predictability and controllability

122

Mouse pox is

Caused by Ectomelia

123

It is not a physical euthanasia method

Cooling in refrigerator

124

a physical euthanasia method

T61
Cervical dislocation
Decapacitation
CO2 inhalation

125

The African clawed for (Xenopus)

Shows mating behaviour after Hcg injection

126

The axolotl is not

Reaches adulthood after a complete metamorphosis

127

The axolotl is

Capable of regenerating lost limbs completely
Carnivorous
Has a long lifespan (8-10 years)

128

Laboratory animals – except the guinea pig – usually adjust their feed intake to satisfy their

Energy requirement

129

Which species can die after oral antibiotic treatment

Guinea pig and rabbit

130

The role of PPAR is to

Burn fats

131

One of the following has no anti-cancer effect

Iron

132

the following has anti-cancer effect:

Resveratrol
CLA
Green tea

133

an important mechanism of epigenomics

Methylation of DNA

134

Zebrafish has a life-span of __year:

6-8
3-5 years according to internet

135

One of them has no role in epigenomics

Change of base in DNA

136

The role of __ is a fine regulation of the protein synthesis in the cell

miRNA / siRNA

137

In some cases the epigenetic changes may be inherited

If the effect(s) acts during the germ-cell proliferation (‘time window’)

138

You can find neoteny at

Axolotl

139

Pheromones are detected by

VNO

140

true about the ferret

We can prevent the constant estrus by using GnRH agonist implants

141

The cephalopods

Are under the protection of the 2010/63/EU Directive since 2013

142

One of the statements is true

Animals in an outbred stock are anisogenic

143

A chromorhnydachyrrhea

Vitamin A deficiency

144

It can go through the uteral wall

Mouse mammary tumour
LCM

145

Carbohydrate ingestion

Stimulate the fatty acid synthase gene expression

146

May increase the mitochondria number

Resveratrol

147

The most used model for drug metabolism study for humans

Macaco

148

Describe the naked mole rat as an animal model.

- Have a gene which conserves collagen.
- Do not age.
- Do not get tumours.
- Life-span of 20-30 years.
- Complex social system and hierarchy.
- Best model for aging and cancer studies

149

Describe the evaluation of the Glires pain categories

- Based on appearance, behaviour, pulse, appetite/drinking, cardiovascular, digestive, and nervous systems.
- 0-4 = Normal
- 5-9 = Needs analgesia
- 10-14 = Moderate pain, possible need to terminate trial
- 15-20 = Not physiological, stop experiment!

150

Explain the pain scoring system for Glires

- 0 points = None
- 1 point = Mild
- 2 points = Moderate
- 3 points = Severe

151

Explain the epigenetics’ mechanism of action

- Modifications e.g. Methylation, histone acetylation, which protect DNA and modify which genes are expressed or silenced.
- Do not change the genome itself
- May be inherited
- Influenced by outside stimuli e.g. nutritional status