Lab exam 03

Question 1

What grows on EMB plates?

Answer 1

Gram positive bacteria

Question 2

Will staphylococcus grow on an EMB plate?

Answer 2

Yes

Question 3

What type of bacteria is used as a lactose fermenter?

Answer 3

Lactococcus and Enterococcus, GRAM NEGATIVE

Question 4

Most pathogens are _____philes

Answer 4

mesophiles

Question 5

What type of bacteria remains after pasteurization

Answer 5

Bacillus

Question 6

Recombination will always alter a cells genotype True or False?

Answer 6

True

Question 7

Mutations that are harmful to cells occur more frequently than those that benefit cells.

Answer 7

True

Question 8

In the Ames test, any colonies that form on the control should be the result of spontaneous mutations.

Answer 8

True

Question 9

According to the operon model, for the synthesis of an inducible enzyme to occur, the

Answer 9

Substrate must bind to the repressor

Question 10

A group of enzymes produced in response to the presence of a substrate is called a(n)

Answer 10

Inducible operon

Question 11

An enzyme that reads DNA to make a molecule of RNA is

Answer 11

RNA polymerase

Question 12

DNA is constructed of

Answer 12

Two strands of nucleotides running antiparallel

Question 13

Which of the following is NOT a product of transcription? tRNA, mRNA, A new strand of DNA, rRNA.

Answer 13

A new strand of dna

Question 14

Conjugation differs from reproduction because conjugation

Answer 14

transfers DNA horizontally, to cells in the same generation.

Question 15

How does decarboxylation affect the pH of the media?

Answer 15

It increases the pH and it becomes more alkaline

Question 16

What is the purpose of peptone in the media?

Answer 16

Provides nutrients and energy

Question 17

The mannitol salt is both ________ and ________.

Answer 17

Selective and differential

Question 18

The autoclave uses ________ to denature proteins.

Answer 18

Coagulation

Question 19

Dry heat uses ______ to kill bacteria.

Answer 19

oxidation

Question 20

Mesophiles prefer the temperature ____ to ____degrees.

Answer 20

20-40

Question 21

Thermophilic bacteria prefer the temperature _____ to _____.

Answer 21

41-122

Question 22

What temp do hyperthermophiles prefer?

Answer 22

60 degrees +

Question 23

What type of bacteria prefer temps at around 20 degrees or less?

Answer 23

Psychrophile

Question 24

Photoautotrophs use

Answer 24

energy from sunlight, carbon dioxide

Question 25

Photoheterotrophs use

Answer 25

light energy as their energy source. They also cannot use carbon dioxide as their sole carbon source.

Question 26

Chemoautotrophs use

Answer 26

inorganic energy sources to synthesize organic compounds from carbon dioxide.

Question 27

Chemoheterotrophs use

Answer 27

are unable to utilize carbon dioxide to form their own organic compounds. Their carbon source is rather derived from sulfur, carbohydrates, lipids, and proteins.

Question 28

Ethanol works best as a disinfectant at 100% concentration. True or False

Answer 28

Fase

Question 29

Halophiles require _____.

Answer 29

Salt

Question 30

Which microorganism is likely to be the cause for a fresh water trout preserved in salt spoiling?

Answer 30

facultative halophiles

Question 31

What happens if you tried to do a direct cell count with 25 colonies?

Answer 31

There is a larger margin for error

Question 32

The Krebs cycle occurs in the _______ in prokaryotes and in the ______ in eukaryotes.

Answer 32

Cytosol in prokaryotes, Mitochondria in eukaryotes

Question 33

Which of the following is a coenzyme?

Answer 33

NADH

Question 34

Genetic change in bacteria can be brought about by transduction conjugation transformation mutation

Answer 34

All are correct

Question 35

Which of the following statements is FALSE? Multiple replication forks are possible on a bacterial chromosome. Prokaryotic cells gets rid of introns in the RNA in the cytoplasm The lagging strand of DNA is started by an RNA primer. DNA polymerase joins nucleotides in one direction only. The leading strand of DNA is made continuously.

Answer 35

DNA polymerase joins nucleotides in one direction only

Question 36

The damage caused by ultraviolet radiation is

Answer 36

cut out and replaced

Question 37

A gene is best defined as

Answer 37

a sequence of nucleotides in DNA that codes for a functional product

Question 38

Transformation is the transfer of DNA from a donor to a recipient cell

Answer 38

As naked DNA in a solution

Question 39

prostate cancer is probably the result of which kind of mutation?

Question 40

The cell membranes of all three domains are composed of straight carbon chains attached to glycerol by ester linkage.

Answer 40

False

Question 41

The highest level in the taxonomic hierarchy is "Kingdom."

Answer 41

False

Question 42

When building a cladogram, the length of the horizontal branch is based on the calculated percent similarity between organisms.

Answer 42

True

Question 43

The deinococci can be categorized as extremophiles.

Answer 43

True

Question 44

All gram-negative bacteria are classified as proteobacteria.

Answer 44

False

Question 45

Which of the following statements about archaea is FALSE?

Answer 45

They evolved before bacteria

Question 46

What is the outstanding characteristic of the Kingdom Fungi?

Answer 46

Members absorb dissolved organic matter

Question 47

Which of the following is NOT based on nucleic-acid hybridization?

Answer 47

Western blotting

Question 48

Which of the following statements is a reason for NOT classifying viruses in one of three domains rather than in a fourth domain?

Answer 48

Viruses are not composed of cells

Question 49

In the scientific name Enterobacter aerogenes, Enterobacter | is the

Answer 49

genus

Question 50

Phage typing is based on the fact that

Answer 50

Bacterial viruses attack only members of a specific species.

Question 51

Nucleic acid hybridization is based on the fact that

Answer 51

Pairing between complementary bases occurs.

Question 52

Which of the following lacks a cell wall?

Answer 52

mycoplasma

Question 53

Plasmogamy, karyogamy, and meiosis are stages of the protozoal sexual life cycle.

Answer 53

False

Question 54

Life cycle of trematodes contain cercariae, metacercaria, redia, and sporocysts stages.

Answer 54

True

Question 55

Both the cellular slime molds and the plasmodial slime molds are members of the phylum Amoebozoa.

Answer 55

True

Question 56

Glycoprotein spikes are found on the capsids of all viruses.

Answer 56

False

Question 57

Viruses are the only known infectious agents that are obligatory intracellular parasites.

Answer 57

False

Question 58

Which of the following statements regarding fungi is FALSE?

Answer 58

Most fungi are pathogenic to humans

Question 59

Which of the following statements regarding lichens is FALSE?

Answer 59

Lichens are very tolerant to pollution

Question 60

Which of the following tends to be more complex in a parasitic helminth than in free-living helminths?

Answer 60

Reproductive system

Question 61

The life cycle of the fish tapeworm is similar to that of the beef tapeworm. Which of the following is the most effective preventive measure?

Answer 61

Cooking fish prior to eating

Question 62

Which of the following is NOT used as a criterion to classify viruses?

Answer 62

Biochemical test

Question 63

Bacteriophages and animal viruses do NOT differ significantly in which one of the following steps?

Answer 63

Biosynthesis

Question 64

A persistent viral infection is one in which

Answer 64

The disease process occurs gradually over a long time

Question 65

An envelope is acquired during which of the following steps?

Answer 65

Release