Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology > Lab Exam > Flashcards

Lab Exam Flashcards

1
Q

What does TSA stand for?

A

tryptic soy agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is a pathogenic organism?

A

disease causing

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

what is an opportunistic pathogen?

A

not normally disease causing but when immune is low will cause disease.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

what is free living organism?

A

lives freely in the environment and not disease causing.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

why are environmental streaks different from quadrant streaks on plate? (3)

A
  • less bacteria then a clinical sample
  • with less bacteria quadrant streaking is ineffective.
  • quadrant streaking is difficult in the field with out access to a flame.
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

what is undefined media?

A

the exact portions and ingredients are unknown

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

what is defined media?

A

the exact portions and ingredients are known

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

what is agar?

A

a seaweed extract

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What is agar used for in TSA plates?

A

used as a solidifier/ gelling agent for TSA and other media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

why is agar better to use then gelatin?

A

agar is firmer and is not degraded by bacteria.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

what are the 3 parameters to sterility? (autoclave)

A
  • temperature
  • time
  • pressure
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Why are the 3 sterility parameters so important?

A

only way to kill fungus spores.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

what are the 6 cultural characteristics used to identify colonies on a plate?

A

size, color, texture, elevation, form, margin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

what 4 characteristics are used to describe broths?

A

surface, amount of growth, color, subsurface,

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

why is staining bacteria important?

A

bacteria are nearly invisible without staining.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

what are 3 common positively charged stains?

A

Methylene blue, safranin, crystal violet

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

what are 2 common negative stains?

A

Eosin, acid fuschin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

how does a stain work? (2 parts)

A
  • Chromophore that colors the bacteria and binds to the Auxochrome
  • Auxochrome that binds to the bacteria.
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

What shape is this bacteria?

A

coccus/cocci

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

What shape is this bacteria?

A

bacillus/bacilli

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

what shape is this bacteria?

A

spirilla/Spirillum

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

What 1D Conformation is this?

A

singles and Pairs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

What 1D Conformation is this?

A

Chains

24
Q

What 1D Conformation is this?

A

Clusters

25
Q

What 1D Conformation is this?

A

Palisades

26
Q

What 3D arrangement is this?

A

Mono

27
Q

What 3D arrangement is this?

A

Diplo

28
Q

What 3D arrangement is this?

A

Staphylo

29
Q

What 3D arrangement is this?

A

Strepto

30
Q

What 3D arrangement is this?

A

Sarcina

31
Q

What 3D arrangement is this?

A

Tetrad

32
Q

what is Pleomorphism?

A

Slight variations in shapes and sizes of bacteria

33
Q

are positive stains heat fixed?

A

Yes

34
Q

Are Negative stains heat fixed?

A

No

35
Q

what is the biggest drawback to positive stains?

A

Deforms the bacteria from heat fixing

36
Q

what is the biggest pro to negative staining?

A

does not distort bactera as there is no hear required.

37
Q

What stain is used for fungus?

A

Lactophenol cotton blue

38
Q

what does ubiquity of micro organisums mean?

A

they are everywhere

39
Q

why is hand washing an important lab technique?

A

prevents bonus bugs on plates

40
Q

what are TSA plates used for?

A

General Bacteria

41
Q

What are R2A plates used for?

A

Aquatic Bacteria

42
Q

What are SAB plates used for?

A

fungus

43
Q

What are GYE plates used for?

A

Actinomycetes

44
Q

what do plates show?

A

general bacterial morphology

45
Q

what are slants used for?

A

maintain cultures in refridgerators for several weeks.

46
Q

what is broth used for?

A

to grow large ammounts of cells

47
Q

what does a Phenol red test provide?

A

if fermintation of sugars is present

48
Q

what does a methyl red test provide?

A

mixed stable acids

49
Q

what does a voges proskauer test provide?

A

butanediol

50
Q

what does citrate test show?

A

if the organsm can use citrate as a carbon source.

51
Q

what does a triple sugar iron test show?

A

if the organism can use different carbohydrates and/or sulfide

52
Q

what does a urea hydrolysis test show?

A

the ability to make urease

53
Q

what does a starch hydrolysis test show?

A

the ability to produce the extra cellular enzyme called alpha-amylase

54
Q

what does a casein test show

A

the ability to make caseinase

55
Q

what does the lipid hydrolysis with spirit blue agar show?

A

ability to make the enzyme lipase

Microbiology (4 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions