Lab Exam 2

Question 1

What stage of a protozoan (cyst or troph) is most likely to be seen in a liquid stool?

Answer 1

troph

Question 1

What are Charcot-Leyden crystals?

Answer 1

artifacts found in stool specimens, spindle shaped structures

Question 2

How soon after collection should a semi-solid or liquid stool be examined?

Answer 2

within 30 minutes of passage

Question 3

Answer 3

Diphyllobothrium latum proglottid

Question 3

Which genera of parasites can be detected and identified by examination of a peripheral blood smear?

Answer 3

Leishmania, Plasmodium, Trypanosoma, Loa, Brugia, and Babesia

Question 3

What is the principle of the zinc-sulfate procedure commonly used for the concentration of a stool specimen?

Answer 3

floatation

Question 3

Why is it useful to perform the direct examination of a stool specimen using iodine?

Answer 3

used to observe cyst of protozoa. Darkens nuclei of cyst of protozoa

Question 4

Answer 4

Ascaris lumbricoides decorticated egg

Question 4

Answer 4

Schistosoma japonicum

Question 4

Answer 4

Taenia saginata proglottid

Question 4

Answer 4

Trichuris trichiura

Question 5

Answer 5

Entamoeba coli troph

Question 6

Answer 6

Balantidium coli troph

Question 7

Answer 7

Entamoeba histolytica cyst

Question 8

Why is it useful to perform the direct examination of a stool specimen using saline?

Answer 8

used to view anything motil, especially helminth eggs

Question 9

Answer 9

Diphyllobothrium latum egg

Question 10

Answer 10

Hymenolepsis dimunata

Question 11

How soon after collection should a formed stool be examined?

Answer 11

macro and microscopic must be done same day, time permitting

Question 12

Answer 12

Diphyllobothrium latum scolex

Question 12

Answer 12

Fasciola/Fasciolopsis

Question 12

Name several specimens other than stools that might be examined for ova and parasites.

Answer 12

Duodenal aspirates, perianal swabs, sputum, urine, blood, tissue biopsy

Question 14

Answer 14

Giardia lamblia cyst

Question 15

Answer 15

Enterobius vermicularis

Question 15

What stage of a protozoan (cyst or troph) is most likely to be seen in a formed stool?

Answer 15

cyst

Question 16

If a formed stool cannot be examined on the day of collection, what steps should be taken?

Answer 16

put specimen in preservative within 30 minutes and refrigerate

Question 17

Answer 17

*Trichinella spiralis* encysted larva

Question 18

Answer 18

*Chilomastix mesnili* troph

Question 20

Answer 20

*Entamoeba coli* cyst

Question 22

Answer 22

*Trichomonas*

Question 23

Answer 23

*Paragonimus westermanii*

Question 24

What does PVA stand for?

Answer 24

poly vinyl alcohol

Question 26

Answer 26

*Endolimax nana* troph

Question 27

What type of container is best to use for the collection and transporting of a stool specimen?

Answer 27

Clean and dry, does not need to be sterile

Question 28

What is the significance of Charcot-Leyden crystals?

Answer 28

indicates inflammation, parasitic infections, or allergic reactions

Question 29

Answer 29

*Iodamoeba bütschlii* troph

Question 29

Name several species of protozoa that have "emerged" as significant pathogens in recent years due to the spread of AIDS.

Answer 29

*Pneumocystis carinii, Cryptosporidium, Toxoplasma gondii*

Question 30

What are Charcot-Leyden crystals thought to be composed of?

Answer 30

ruptured eosinophil granules

Question 32

Answer 32

*Dientamoeba fragilis*

Question 34

Answer 34

*Necator americanus* (hookworm) rhabditiform larva

Question 35

What organism is the scotch tape preparation used for?

Answer 35

*Enterobius vermicularis* (pinworms)

Question 36

Answer 36

*Trypanosoma*

Question 37

Answer 37

*Schistosoma mansoni*

Question 38

Answer 38

*Iodamoeba bütschlii* cyst

Question 38

Answer 38

*Clonorchis sinensis*

Question 39

Answer 39

*Ascaris lumbricoides* unfertilized egg

Question 39

Answer 39

*Strongyloides stercoralis* rhabditiform larva

Question 41

Answer 41

*Taenia solium* scolex

Question 42

Name several artifacts that can be found in ova and parasite preparations.

Answer 42

plant fibers, pollen grains, meat fibers, epithelial cells, Charcot-Leyden crystals, and WBCs

Question 43

Name the two procedures commonly used for the concentration of a stool specimen?

Answer 43

Formalin-ethyl acetate and zinc-sulfate

Question 44

Answer 44

*Ascaris lumbricoides* fertilized egg

Question 46

What is the principle of the formalin-ethyl acetate procedure commonly used for teh concentration of a stool specimen?

Answer 46

sedimentation

Question 47

Answer 47

*Taenia solium* proglottid

Question 49

Answer 49

*Hymenolepsis nana*

Question 51

Answer 51

*Balantidium coli* cyst

Question 52

Answer 52

*Hymenolepsis dimunata*

Question 54

Answer 54

*Chilomastix mesnili* cyst

Question 55

Answer 55

*Taenia saginata* scolex

Question 56

Answer 56

*Necator americanus* (hookworm) egg

Question 58

Answer 58

*Schistosoma haematobium*

Question 60

Answer 60

*Giardia lamblia* troph

Question 61

Answer 61

*Hymenolepsis nana*

Question 62

Answer 62

*Hymenolepsis dimunata*

Question 63

Answer 63

*Entamoeba histolytica* troph

Question 64

What fixative should be used with a liquid stool?

Answer 64

PVA

Question 65

Answer 65

*Taenia saginata* egg

Question 66

What fixative should be used with a formed stool?

Answer 66

10% formalin

Question 67

What are teh four components of the procedure for the laboratory examination of a stool specimen for ova and parasites?

Answer 67

Macroscopic exam, direct microscopic exam, concentration, permanent stain smear

Question 68

Answer 68

*Endolimax nana* cyst