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MT6320 > [LAB] Exercise 10A: Throat Culture > Flashcards

[LAB] Exercise 10A: Throat Culture Flashcards

(61 cards)

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1
Q

A diagnostic test to detect presence of microorganisms in the upper respiratory tract

A

Throat culture/Throat Swab

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2
Q

The most important pathogen causing strep throat

A

Streptoccocus pyogenes

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3
Q

What microorganism can also be recovered and is associated with tonsillar abscess?

A

Staphyloccocus aureus

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4
Q

What other indigenous flora can be detected in throat culture?

A

Viridans streptococci
Beta hemolytic strep (Group A,B,C)
Staphyloccoci
Haemophilus
Moraxella
Anaerobic Bacteria (Clostridium spp.?)

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5
Q

Gram-negative coliform bacteria that is also detected in throat culture

A

Klebsiella

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6
Q

A sterile tongue depressor is used to apply pressure on the tongue to visualize what?

A

tonsillar fossae and posterior pharynx

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7
Q

In the collection of throat swab specimens, 1-2 sterile swabs is extended between?

A

Tonsillar pillars (golden arches) and behind the uvula

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8
Q

Which sites should we avoid in collecting throat swab specimen?

A

Tongue
Buccal mucosa
Uvula

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9
Q

Rub the sterile swabs back and forth across the?

A

Posterior pharynx
Tonsillar areas
Any inflamed or exudative areas

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10
Q

T/F: Do not collect specimens if the epiglottis is inflamed

A

True

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11
Q

Collecting specimens from an inflamed epiglottis can lead to?

A

Respiratory obstruction

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12
Q

Where are swabs placed after collection?

A

Sterile tube or transport media

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13
Q

T/F: All culture media (BAP, CAP, PEA, EMB) should be brought at 37C prior to inoculation

A

False
Room temperature

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14
Q

Firmly roll the swab on to BAP over ____ portion of the BAP surface

A

1/6th

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15
Q

T/F: Using the same swab from BAP, the swab is rolled as well into CAP, PEA, and EMB.

A

True

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16
Q

What streaking method is used?

A

Isolation/four quadrant method

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17
Q

Where is the Taxo A disk placed in the inoculated BAP?

A

Junction of 1st and 2nd quadrants

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18
Q

How much is the distance of Taxo A disk from the edge?

A

10-mm from the edge

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19
Q

What is the component of Taxo A Disk?

A

0.04 units Bacitracin

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20
Q

Inoculated plates are incubated at what temperature?

A

37C

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21
Q

What is the incubation time for the inoculated plates?

A

18-24 hours

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22
Q

Growth can be further enhanced if the inoculated BAP and CAP are incubated with?

A

Increased CO2

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23
Q

What can be done to improve detection of hemolysis?

A

After streaking, carefully stab the BAP several times using the same loop in the streaked and unstreaked areas

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24
Q

In which media can we observe for isolated pinhead colonies and pinpoint colonies that are entire, white or yellow, clear or opalescent, and creamy in appearance?

A

BAP

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25
T/F: Other colonies that may be present include large, grayish mucoid/slimy colonies
True
26
In which media can we observe colonies that are similar to BAP but no hemolytic patterns?
CAP
27
T/F: We also observe for pinpoint and pinhead colonies in PEA
True
28
Colonies growing on this medium is presumptive as gram-positive cocci
PEA
29
T/F: Growth is usually seen in EMB
False; No growth is usually seen
30
In which media can we observe large purple or colorless colonies if growth is seen
EMB
31
T/F: It is susceptible if there is no zone of inhibition
False
32
Interpretation if there is any zone of inhibition
Susceptible
33
Interpretation if there is no zone of inhibition
Resistant
34
In the Catalase Test, from which media do we fish 1-2 similar looking isolated pinhead and pinpoint colonies?
PEA or CAP
35
In the Catalase Test, what is added to the slides mixed with the colonies?
1-2 drops of 3% Hydrogen Peroxide
36
Positive result of Catalase Test
Bubbling or effervescence
37
In which media is the colony inoculated from in Gram stain?
BAP or CAP
38
T/F: Proceed to the succeeding procedure if you obtained a negative catalase test
False Do not proceed
39
T/F: If you obtained a positive catalase test, proceed to Coagulase Testing
True
40
What media is the specimen inoculated from in coagulase test?
BAP or CAP
41
In the slide coagulase test what is first dropped on a clean grease-free slide?
NSS
42
After emulsifying colonies in slide coagulate testing, what is added next?
1-2 drops of rabbit plasma
43
T/F: In the slide coagulase test, the slide is placed on a dark/black cardboard and tilted when observing for macroscopic clumping
True
44
How long do we observe for macroscopic clumping in slide coagulase test
Around 10 seconds
45
When is the test considered negative for Slide Coagulate Test?
No clumping occurs after 2 minutes
46
What test tube is used for Tube Coagulase Test?
Kahn test tube
47
In tube coagulase test, how much rabbit plasma is placed on the Kahn test tube?
0.5mL
48
T/F: Pooled human plasma can be used if rabbit plasma is unavailable in Tube Coagulase Test
True
49
For how long should we incubate the tube coagulase test sample when observing for clots/coagulum?
4 hours
50
In what temperature should the tube coagulase test be incubated at?
37C
51
What is the positive result for tube coagulase test?
Clot or coagulum formation
52
If no clot is seen after 4 hours, what should be done before reporting the tube coagulase test as negative?
Incubate at room temperature for 20 hours
53
In Mannitol Fermentation Test, from what media are the 2-3 colonies fished out from?
PEA or BAP
54
In Mannitol Fermentation Test, what streaking method is used?
Simple streaking
55
In Mannitol Fermentation Test, what is the incubation time and temperature?
24 hours, 37C
56
Mannitol Fermenter/Non-Mannitol Fermenter: Colonies are yellow and plate becomes yellow
Mannitol Fermenter
57
Mannitol Fermenter/Non-Mannitol Fermenter: Pink to red plates
Non-mannitol fermenter
58
Other tests can be performed on which colonies?
Catalase negative and are gram positive cocci in chains
59
What is resistant to Taxo P (Optochin)?
Viridans streptococci (alpha hemolytic)
60
T/F: S. pyogenes is PYR postive
True
61
T/F: S. pyogenes is susceptible to SXT-TMP
False Resistant

MT6320 (8 decks)

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