Lab final

Question 1

What is the graph axis in pipette calibration?

Answer 1

Graph of volume delivered vs volume nominal

Question 2

What must be true about the graph?

Answer 2

It must have 3 or more points.

Question 3

Can the graph be used to establish a linear relationship?

Answer 3

No!

Question 4

What kind of graph/equation do we use in order to understand a pipette calibration?

Answer 4

A linear, Y= MX+B equation

Question 5

When working towards a unknown concentraton in ug/uL, what are the steps?

Answer 5

1.) Use the Abs of the unknown as the Y
2.) Solve for X
Divide x by the uL used.

Question 6

With buffers, what is the equation when a weak acid and strong base (I.E. Acetic acid and NaOH)

Answer 6

HA + OH = A- + H2O

Question 7

With buffers, what is the equation when a weak base and strong acid (I.E. Sodium acetate and HCl)

Answer 7

H+ + A- = HA

Question 8

What is the equation for total parts?

Answer 8

10 ^ Ph-PKA +1

Question 9

How do you determine the total moles?

Answer 9

Molarity * Volume

Question 10

How do you get your moles of HA or A-?

Answer 10

Total moles/Total Parts

Question 11

How do you get A- or OH?

Answer 11

Total Moles - (Total moles/Total Parts)

Question 12

What is mole of HA equal to? Mole of A-?

Answer 12

HCL and NaOH respectively.

Question 13

What is the first and second step of paper chromotography?

Answer 13

Spot standard and the unknown, then put in cylinder shape and into solvent.

Question 14

Is the paper the stationary or mobile phase? What would be the other one?

Answer 14

The Paper is the stationary phase, while the solvent is the mobile phase.

Question 15

What type of amino acids travel the the furthest?

Answer 15

Non Polar (LWAGPIVMF)

Question 16

What type of amino acids will travel the second furthest?

Answer 16

STYCQN (Polar-Neutral AA)

Question 17

What type of AA will travel the least?

Answer 17

The polar AA (ED, HRK)

Question 18

What are the 3rd and 4th steps of paper chromatography?

Answer 18

Let it run through the whole paper, and do a post spot run, and let that dry.

Question 19

What is the final 3 steps of paper chromeotography?

Answer 19

Spray with ninhydrin (detect AAs), Place in oven to speed up development (3 to 5 mins), and read.

Question 20

What’s the qualitative measure of paper chromatography? What is the quantitative measure?

Answer 20

Quali: Color based, Quant: rF value (Distance sample/Distance solvent).

Question 21

In gel filtration/SDS PAGE, what is the mode of serpation?

Answer 21

Size (specifically molecular weight)

Question 22

What’s the order of elution in gel filtration?

Answer 22

Largest to smallest

Question 23

Whats the order of elution for SDS page?

Answer 23

Smallest (travel down the gel further) -> largest.

Question 24

With Enzyme kinetics, what does the M-M plot estimate?

Answer 24

Km and Vmax

Question 25

How does a Lineweaver burke plot differ from the MM plot?

Answer 25

It allows us to calculate the Km and Vmax.

Question 26

How do you calculate the starting and ending concentrations or volumes?

Answer 26

C1V1 = C2V2

Question 27

What Enzyme was used in the reaction?

Answer 27

L-DOPA

Question 28

How do you name the primary antibody in western blot?

Answer 28

Rabbit/Mouse - anti - target protein

Question 29

How do you name the secondary antibody in western blotting?

Answer 29

Animal used - anti - 1st animal - Conjugate enzyme (AP/HRP)

Question 30

What is the order of western blotting?

Answer 30

Transfer, optional wash, block wash, primary antibody, wash, secondary antibody, wash, and develop.

Question 31

What does NaCl do in DNA discovery?

Answer 31

Helps with participation process for the DNA/Removes proteins attached to DNA