Lab Final Exam Part II (Ex 9 and 10) Flashcards
These are small extra-chromosomal elements that can exist in some bacteria
Plasmids
The bacterial genome is made up of ___ and ___ (if the bacteria contain them)
Bacterial chromosomes + plasmids
What do plasmids often carry that can be conferred to the bacteria?
Genes for antibiotic resistance
True/False: Plasmids can be very useful for bacteria
True. Plasmids can be very useful for bacteria.
This kind of plasmid may have up to 300 copies of itself in any one bacterial cell.
High copy number
If a plasmid within a bacterial cell contains 300 copies of itself in one bacterial cell, how many copies of its gene product can it produce?
300 copies
Why type of genes goes the [plasmid] pGLO contain within it? (2)
ampicillin resistance gene, gene for GFP (green fluorescence protein)
What does the ampicillin resistance gene code for?
The exoenzyme ampicillinase
How are exoenzymes released?
They are secreted into the environment
What is secreted from E. coli that has been inserted with pGLO, and what does it cause?
Ampicillinase is secreted into the environment and proceeds to destroy the ampicillin the media.
What within an organism, if present, can block insertion of a plasmid, causing it to be cut up?
Restriction enzymes
What is the name of the inducer in the Biorad kit for the pGLO plasmid?
arabinose (sugar)
How goes arabinose act on the pGLO plasmid?
It “turns on” the glow portion of it
What is the purpose of heat shocking the sample?
It opens the pores
What does CaCl2 do, and what step in the procedure process facilitates it to happen?
Incubating on ice allows the DNA to attach to the CaCl2 and bacterial cell walls; The CaCl2 brings the plasmids close to the cell, so that it can made entry when the pores are opened by heat shocking them.
