[Lab] Flagellates Part 2

Question 1

Does the Trichomonas spp. have a cyst stage

Answer 1

No

Question 2

True or false: Trichomonas spp. has a rounded anterior end and somewhat pointed
posterior end.

Answer 2

True

Question 3

True or false: Trichomonas spp. has a straight and soft axostyle

Answer 3

False (curved and rigid)

Question 4

True or false: Trichomonas spp. has a small cytostome on one side of the
anterior end.

Answer 4

True

Question 5

Shape of nuclei of Trichomonas spp.

Answer 5

Spherical

Question 6

Trichomonas spp. has a blepharoplast

Answer 6

Yes

Blepharoplast is present margin from which 3-5
flagella and a marginal flagellum on an undulating membrane, the base attachment being a heavy costa.

Question 7

Size of T. vaginalis trophozoite

Answer 7

15-20

Question 8

Shape of T. vaginalis trophozoite

Answer 8

Pyriform

Question 9

True or false: the axostyle of T. vaginalis trophozoite is prominent

Answer 9

True

Question 10

Number of flagella of T. vaginalis trophozoite

Answer 10

4 (all are anterior)

Question 11

What will you see in the cytoplasm of T. vaginalis trophozoite

Answer 11

Siderophil granules

Question 12

Motility of T. vaginalis trophozoite

Answer 12

Jerky (tumbling), fast

Question 13

Where does the T. vaginalis live in a male host

Answer 13

Epithelium of the urethra, prostate gland

Question 14

Where does the T. vaginalis live in a female host

Answer 14

Surface of vaginal epithelium

Question 15

True or false: Male hosts of T. vaginalis are always symptomatic

Answer 15

False (usually asymptomatic carriers)

Question 16

MOT of T. vaginalis

Answer 16

1. Sexual intercourse
2. Contaminated toilet seat and towel

Question 17

True or false: T. vaginalis is the only pathogenic Trichomonas

Answer 17

True

Question 18

Disease caused by T. vaginalis

Answer 18

Trichomoniasis

Question 19

Clinical manifestations of T. vaginalis

Answer 19

1. Inflamed vaginal walls with petechial hemorrhage.
2. Surface of the vaginal epithelium is covered with coagulated materials: leukocytes, RBC and Trichomonas
3. Desquamation of the epithelial cells
4. Cause non-specific urethritis, vaginitis and prostatitis.

Question 20

Signs and symptoms of Trichomoniasis

Answer 20

1. Greenish yellow frothy vaginal
   discharge
2. Intense itchiness of the vagina
3. Burning sensation
4. Strawberry cervix

Question 21

Technique/s used for the recovery of T. vaginalis

Answer 21

1. Unstained saline wet preparation (TROPHOZOITE)

Question 22

Specimen of interest for T. vaginalis (male)

Answer 22

a. Semen
b. Prostatic secretions

Question 23

Specimen of interest for T. vaginalis (female)

Answer 23

a. Urine
b. Urethral discharges
c. Vaginal discharges
d. Cervical secretion

Question 24

Stains used for recovery of T. vaginalis

Answer 24

1. Giemsa
2. Pap’s
3. Romanowsky
4. Acridine Orange

Question 25

Culture media for T. vaginalis

Answer 25

a. Diamond’s modified medium b. Feinberg and Whittington culture media c. Cysteine Peptone Liver Maltose d. Simplified Trypticase Serum

Question 26

Treatment for T. vaginalis

Answer 26

1. Oral metronidazole (both sexes) 2. Acid douche (females)

Question 27

Prevention and control for T. vaginalis

Answer 27

1. Proper personal hygiene . 2. Avoid promiscuous sexual intercourse. 3. Protective devices such as condoms should be used.

Question 28

Size of T. tenax trophozoite

Answer 28

5-12

Question 29

No. of flagella of T. tenax trophozoite

Answer 29

4

Question 30

Does T. tenax have an undulating membrane

Answer 30

Yes

Question 31

Does T. tenax lack a free posterior extension

Answer 31

Yes

Question 32

T. tenax lives in

Answer 32

Oral cavity

Question 33

MOT of T. tenax

Answer 33

Droplet spray from the mouth (Kissing, common use of contaminated dishes and drinking glasses)

Question 34

Lab diagnosis of T. tenax

Answer 34

1. Swabbing the tartar between gingival margin of the gums or the tonsillar crypts. 2. Direct examination of mouth scrapings.

Question 35

Size of T. hominis trophozoite

Answer 35

7-13

Question 36

No. of flagella of T. hominis trophozoite

Answer 36

5 (anterior)

Question 37

Where does T. hominis live

Answer 37

Colon

Question 38

MOT of T. hominis

Answer 38

Ingestion of contaminated food and water