Lab Midterms

Question 1

Other name for paroxysmal nocturnal hemoglobinuria

Answer 1

Marchiafava-Micheli Syndrome

Question 2

Rare and potentially life threatening disease of the blood characterized by complement induced hemolytic anemia

Answer 2

PNH

Question 3

How is sleeping associated with PNH?

Answer 3

Blood pH becomes acidic during sleep which leads to hemolysis of RBC

Question 4

Screening test in which px RBCs are placed in low-ionic strength solution and observed for hemolysis

Answer 4

Sugar or sucrose lysis test

Question 5

Specific test for PNH

Answer 5

Ham’s acid hemolysis

Question 6

He described the test for PNH in 1937

Answer 6

Dr. Thomas Ham

Question 7

Gold standard, rapid and sensitive test for PNH

Answer 7

Flow cytometry

Question 8

Reagent added in Ham’s acid hemolysis test

Answer 8

0.5ml of 0.2N HCl

Question 9

Another term for sickle cell disease/anemia

Answer 9

Drepanocytosis

Question 10

Genetic blood disorder characterized by abnormal, rigid and sickled RBCs

Answer 10

Sickle cell disease/anemia or drepanocytosis

Question 11

Chemical deoxygenizers

Answer 11

2% sodium metabisulfite
2% sodium sodium dithionite
2% ascorbic acid

Question 12

Best method for sickle cell determination

Answer 12

Daland and Da Silva Method

Question 13

Presence of sickle hemoglobin can be demonstrated with

Answer 13

Sickle solubility test

Question 14

Tests for sickle cell anemia determination

Answer 14

Daland and Da Silva

Sherman’s test

Question 15

Test that follows the law of osmosis, in which there is a transfer of solution from lower to higher concentration

Answer 15

EOFT

Question 16

RBC + hypertonic solution = ?

Answer 16

Shrinking/crenating

Question 17

RBC + hypotonic solution = ?

Answer 17

Swelling/bursting

Question 18

These cells are fragile due to increased osmotic fragility index

Answer 18

Spherical cells

Question 19

Resistant cell due to low osmotic fragility index

Answer 19

Sickle cell

Question 20

Hemolysis of normal cells begin in ? % salt solution

Answer 20

0.39-0.45% salt solution

Question 21

Complete hemolysis of normal cells occur in ? % salt solution

Answer 21

0.30-0.33% salt solution

Question 22

This test’s principle is on the stability of red cells under different concentration of hypotonic sodium chloride solutions

Answer 22

Sanford method

Question 23

Automated method for EOFT

Answer 23

Fragillograph

Question 24

Blood is diluted in hypotonic saline and buffered to pH 7.4 wih phosphate

Answer 24

Dacie’s method

Question 25

Conditions that cause increased EOFT

Answer 25

Hemolytic anemia | Spherocytosis

Question 26

In the case of spherocytosis, IH occurs at ? and CH at ?

Answer 26

``` IH = tube 24 CH = tube 20 ```

Question 27

Severe IDA: IH and CH?

Answer 27

``` IH = tube 19 CH = tube 15 ```

Question 28

Standard factor for concentration of NaCl

Answer 28

0.02

Question 29

Protects cell from complement

Answer 29

CD59

Question 30

For early complement activation

Answer 30

CD55

Question 31

Protein absent in PNH

Answer 31

GPI Protein

Question 32

Test for cold hemolysin

Answer 32

Donath Landsteiner

Question 33

Cold Ab in Donath Landsteiner is associated with this blood group system

Answer 33

P Blood group system

Question 34

What Ig class is a Donath Landsteiner cold hemolysin?

Answer 34

IgG

Question 35

Principle of Donath Landsteiner

Answer 35

Raynaud's phenomenon

Question 36

Stain for differentiation of AML from ALL, stains granules black

Answer 36

Sudan Black B

Question 37

Helpful in diagnosing erythroleukemia

Answer 37

Periodic acid schiff

Question 38

Differentiates myeloblastic and monoblastic leukemias from lymphoblastic leukemias

Answer 38

Periodic acid schiff

Question 39

Basic fuchsin dye in PAS

Answer 39

Schiff's reagent

Question 40

Color of PAS stain

Answer 40

Magenta to purple

Question 41

Other term for specific esterase

Answer 41

Naphthol AS-D Chloroacetate

Question 42

Granules of myeloblasts stain blue-black Stains neutrophilic granulocytes Differentiates monoblasts and myeloblasts

Answer 42

Specific esterase

Question 43

Other term for nonspecific esterase

Answer 43

Alpha-Naphthyl Acetate

Question 44

Added to inhibit activity of nonspecific esterase in monocytes

Answer 44

Na fluoride