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1

Other name for paroxysmal nocturnal hemoglobinuria

Marchiafava-Micheli Syndrome

2

Rare and potentially life threatening disease of the blood characterized by complement induced hemolytic anemia

PNH

3

How is sleeping associated with PNH?

Blood pH becomes acidic during sleep which leads to hemolysis of RBC

4

Screening test in which px RBCs are placed in low-ionic strength solution and observed for hemolysis

Sugar or sucrose lysis test

5

Specific test for PNH

Ham's acid hemolysis

6

He described the test for PNH in 1937

Dr. Thomas Ham

7

Gold standard, rapid and sensitive test for PNH

Flow cytometry

8

Reagent added in Ham's acid hemolysis test

0.5ml of 0.2N HCl

9

Another term for sickle cell disease/anemia

Drepanocytosis

10

Genetic blood disorder characterized by abnormal, rigid and sickled RBCs

Sickle cell disease/anemia or drepanocytosis

11

Chemical deoxygenizers

2% sodium metabisulfite
2% sodium sodium dithionite
2% ascorbic acid

12

Best method for sickle cell determination

Daland and Da Silva Method

13

Presence of sickle hemoglobin can be demonstrated with

Sickle solubility test

14

Tests for sickle cell anemia determination

Daland and Da Silva
Sherman's test

15

Test that follows the law of osmosis, in which there is a transfer of solution from lower to higher concentration

EOFT

16

RBC + hypertonic solution = ?

Shrinking/crenating

17

RBC + hypotonic solution = ?

Swelling/bursting

18

These cells are fragile due to increased osmotic fragility index

Spherical cells

19

Resistant cell due to low osmotic fragility index

Sickle cell

20

Hemolysis of normal cells begin in ? % salt solution

0.39-0.45% salt solution

21

Complete hemolysis of normal cells occur in ? % salt solution

0.30-0.33% salt solution

22

This test's principle is on the stability of red cells under different concentration of hypotonic sodium chloride solutions

Sanford method

23

Automated method for EOFT

Fragillograph

24

Blood is diluted in hypotonic saline and buffered to pH 7.4 wih phosphate

Dacie's method

25

Conditions that cause increased EOFT

Hemolytic anemia
Spherocytosis

26

In the case of spherocytosis, IH occurs at ? and CH at ?

IH = tube 24
CH = tube 20

27

Severe IDA: IH and CH?

IH = tube 19
CH = tube 15

28

Standard factor for concentration of NaCl

0.02

29

Protects cell from complement

CD59

30

For early complement activation

CD55

31

Protein absent in PNH

GPI Protein

32

Test for cold hemolysin

Donath Landsteiner

33

Cold Ab in Donath Landsteiner is associated with this blood group system

P Blood group system

34

What Ig class is a Donath Landsteiner cold hemolysin?

IgG

35

Principle of Donath Landsteiner

Raynaud's phenomenon

36

Stain for differentiation of AML from ALL, stains granules black

Sudan Black B

37

Helpful in diagnosing erythroleukemia

Periodic acid schiff

38

Differentiates myeloblastic and monoblastic leukemias from lymphoblastic leukemias

Periodic acid schiff

39

Basic fuchsin dye in PAS

Schiff's reagent

40

Color of PAS stain

Magenta to purple

41

Other term for specific esterase

Naphthol AS-D Chloroacetate

42

Granules of myeloblasts stain blue-black
Stains neutrophilic granulocytes
Differentiates monoblasts and myeloblasts

Specific esterase

43

Other term for nonspecific esterase

Alpha-Naphthyl Acetate

44

Added to inhibit activity of nonspecific esterase in monocytes

Na fluoride