Lab Practical 2 Flashcards
Oxidase test
What color is positive for oxidase?
Blue or purple within 15 seconds
Oxidase test
2 organisms used?
What was + and -?
*pseudomonas aeruginosa (+)
*escherichia coli (-)
Hydrolysis of starch
What color indicates positive for AMYLASE?
What color indicates negative?
(+) Iodine color around streak (showed yellow on lab)
(-)dark purple around streak
Hydrolysis of starch
What bacteria were used for test?
What was + and -?
Escherichia coli was (-)
Bacillus spizizenii (-)
Hydrolysis of gelatin
What indicates positive and negative appearance of gelatinase?
Liquid gelatin medium/tube (+)
Solid gelatin medium/tube (-)
Hydrolysis of gelatin
What bacteria were used and what was + and - for gelatinase ?
Bacillus spizizenii (+)
Escherichia coli (-)
Detection of catalase
What indicates a positive and negative appearance?
bubble (+)
No bubble (-)
Detection of catalase
What organisms were used and what was + and -?
Staphylococcus aureus (+)
Streptococcus equisimilis (-)
Hydrolysis of urea
What colors indicate positive and negative appearance ?
What is produced for +?
Positive is hot pink
Negative is yellow/orange
Urease
Hydrolysis of urea
What is the indicator used ?
Phenol red
Hydrolysis of urea
What organisms were used and what is + and -?
Proteus mirabilis (+)
Escherichia coli (-)
Fermentation of carbohydrates
What indicates a positive and negative appearance or no change ?
Positive is YELLOW indicating acid
BUBBLE indicates positive for gas
Negative is PINK (basic) or red indicating no change
Fermentation of carbohydrates
What 4 types of carbohydrates can be used ?
Glucose
Sucrose
Lactose
Mannitol
Fermentation of carbohydrates
What 3 organisms were used and what were the results ?
Escherichia coli (+) for acid and gas
Alcaligenes faecalis (-) no change
Staphylococcus aureus (+) for acid only , no gas
S. I. M.
What does this test for ?
Sulfur- (production of hydrogen sulfide)
Indole- (production of indole)
Motility-(ink dictation of organism motility)
What indicates positive appearance for S.I.M. Test ?
What reagent used for (I)?
(S) - black precipitate (iron sulfide)
(I) - red reagent layer (Kovacs reagent)
(M)- turbidity / spreading away from stab line
S.I.M. Test
What organisms were used and which were positive ?
Escherichia coli S(-), I(+), M(+)
Citrobacter Freundii S(+), I(-), M(+)
MRVP
Positive and negative appearance for test?
Red (+)
Yellow (-)
MRVP
What 2 bacteria were used and which is - and +?
Enterobacter aerogenes (-) for MR, (+) for VP
Escherichia Coli (+) for MR, (-) for VP
MRVP
What are the indicators for MR and VP?
MR - methyl red
VP - VP A & B
SImmons citrate test
What colors are positives and negative?
Blue is (+)
Green is (-)
Simmons citrate test
What organisms were used and what results ?
Escherichia coli (-)
Enterobacter aerogenes (+)
