Lab Practicum 2 Protozoan Table

Question 1

Entamoeba histolytica - disease it causes

Answer 1

Amebic dysentery
- Diarrhea

Question 2

Entamoeba histolytica - infective stage and mode of transmission

Answer 2

Transmitted through fecal and oral route

• Quadranucloate cyst (infections)
• Ingestion (hatches in small intestine)

Question 3

Entamoeba histolytica - mode of motility

Answer 3

Pseudopod

Question 4

Entamoeba histolytica - symptoms

Answer 4

Bloody diarrhea (penetrate lining of intestine and causes ulcers)

Question 5

Entamoeba histolytica - treatment

Answer 5

Metronidazole

Question 6

Entamoeba histolytica - method of diagnosis

Answer 6

• Some people are asymptomatic carriers (only 10-20% of infected people)
• Stool sample to look for cysts or trophs

Question 7

Entamoeba histolytica - stages in the life cycle

Answer 7

Cysts - up to 4 nuclei
Trophs - 8 trophs

Question 8

Balantidium coli - disease it causes

Answer 8

Balantidiosis
- diarrhea

Question 9

Balantidium coli - infective stage and mode of transmission

Answer 9

Fecal and oral transmission
- Eating contaminated food or drinking water

Question 10

Balantidium coli - mode of motility

Answer 10

cilia

Question 11

Balantidium coli - symptoms

Answer 11

Often asymptomatic but can have bloody diarrhea, weight loss, stomach pain, etc.

Question 12

Balantidium coli - treatment

Answer 12

metronidazole

Question 13

Balantidium coli - method of diagnosis

Answer 13

Stool sample looking for trophs or cysts in stool sample

Question 14

Balantidium coli - stages in the life cycle

Answer 14

Cyst - kidney shaped nuclei
Troph - oval

Question 15

Giardia lamblia (intestinalis) - disease it causes

Answer 15

Giardiasis
- Backpacker’s diarrhea

Question 16

Giardia lamblia (intestinalis) - infective stage and mode of transmission

Answer 16

Beavers are carriers and shed cysts in the water during a bowel movement

Question 17

Giardia lamblia (intestinalis) - mode of motility

Answer 17

flagella

Question 18

Giardia lamblia (intestinalis) - symptoms

Answer 18

Watery diarrhea, fatigue, nausea, flatulence, weakness, weight loss, and abdominal cramps

Question 19

Giardia lamblia (intestinalis) - treatment

Answer 19

metronidazole

Question 20

Giardia lamblia (intestinalis) - method of diagnosis

Answer 20

Stool sample to verify cysts and trophs

Question 21

Giardia lamblia (intestinalis) - stages in the life cycle (cysts, troph, etc)

Answer 21

cysts - up to 4 nuclei
Troph - 2 nuclei + 4 pairs of flagella

Question 22

Trichomonas vaginalis - disease it causes

Answer 22

Trichnomiasis (STI)

Question 23

Trichomonas vaginalis - infective stage and mode of transmission

Answer 23

Sexually transmitted infection

Question 24

Trichomonas vaginalis - mode of motility

Answer 24

flagella

Question 25

Trichomonas vaginalis - symptoms

Answer 25

Many people asymptomatic Itching, burning, redness, discomfort w/ urination, change in vaginal discharge

Question 26

Trichomonas vaginalis - treatment

Answer 26

metronidazole

Question 27

Trichomonas vaginalis - method of diagnosis

Answer 27

Molecular testing and wet mount of discharge fluid to look for troph

Question 28

Trichomonas vaginalis - stages in the life cycle

Answer 28

Troph - large nucleus w/ 4 flagella

Question 29

Trypanosoma brucei - disease

Answer 29

African sleeping sickness

Question 30

Trypanosoma brucei - infective stage and mode of transmission

Answer 30

Intermediate host: tsetse fly - asexual part of life cycle Definitive host: sexually transmitted infection

Question 31

Trypanosoma brucei - mode of motility

Answer 31

undulating membrane

Question 32

Trypanosoma brucei - symptoms

Answer 32

fever, headaches, muscle and joint aches, and fatigue

Question 33

Trypanosoma brucei - treatment

Answer 33

pentamidine

Question 34

Trypanosoma brucei - method of diagnosis

Answer 34

finding parasite in body fluids or tissue by microscopy

Question 35

Trypanosoma brucei - stages in the life cycle

Answer 35

Trypomastigote - definitive host Epimastigote - intermediate host

Question 36

Toxoplasma gondii - disease

Answer 36

Toxoplasmosis

Question 37

Toxoplasma gondii - infective stage and mode of transmission

Answer 37

consuming food or water contaminated by cat feces or contaminated environmental samples - injestion of contaminated meat - from mother to child in utero

Question 38

Toxoplasma gondii- mode of motility

Answer 38

undulating membrane

Question 39

Toxoplasma gondii - symptoms

Answer 39

usually asymptomatic - mild flu like symptoms - still birth, encephalitis

Question 40

Toxoplasma gondii - treatment

Answer 40

pyrimethamine sulfadiazine

Question 41

Toxoplasma gondii - method of diagnosis

Answer 41

serological tests looking for antibody production

Question 42

Toxoplasma gondii - stages in the life cycle

Answer 42

Troph

Question 43

Plasmodium vivax - disease

Answer 43

malaria

Question 44

Plasmodium vivax - infective stage and mode of transmission

Answer 44

definitive host: cysts in mosquito gut wall -> sporozoites in mosquito saliva intermediate host: merozoites released by liver

Question 45

Plasmodium vivax- mode of motility

Answer 45

gliding

Question 46

Plasmodium vivax - symptoms

Answer 46

chills, nausea, vomiting and headaches, then fever

Question 47

Plasmodium vivax - treatment

Answer 47

quinine, chloroquine, mefloquine

Question 48

Plasmodium vivax - method of diagnosis

Answer 48

looking for parasites in the blood, usually by microscopy

Question 49

Plasmodium vivax - stages in the life cycle

Answer 49

sporozoite: infective to humans merozoite ring stage schizont: multi-nucleic cell