Lab Quiz 1

Question 1

acid has a blank charge

Answer 1

negative

Question 2

bacteria has a blank charge

Answer 2

negative

Question 3

acid will stain blank

Answer 3

background

Question 4

base will stain blank

Answer 4

bacteria

Question 5

basic has a blank charge

Answer 5

positive

Question 6

two types of staining

Answer 6

simple, differential

Question 7

staining technique that uses single basic dye that allows one to see shapes, arrangements, and relative size

Answer 7

simple

Question 8

staining technique that shows differences between bacteria or within bacteria

Answer 8

differential

Question 9

purple from crystal violet turns blank purple and is blank

Answer 9

staph aureus cocci, positive

Question 10

red from safranin turns blank red and is blank

Answer 10

e. coli rods, negative

Question 11

using iodine does what in gram positive

Answer 11

intensifies the color

Question 12

decolorization can be done using blank

Answer 12

alcohol decolorizing solution

Question 13

this stain uses acid dye and stains background

Answer 13

negative stain

Question 14

negative stain is not blank so it shows true size

Answer 14

heat fixed

Question 15

negative stain shows the blank

Answer 15

capsule

Question 16

this is a black dye that produces a purple background color

Answer 16

nigrosin

Question 17

white rods that result from negative staining

Answer 17

B. cereus

Question 18

differential staining that is heat fixed, uses two types of bacteria, and needed steaming

Answer 18

acid-fast

Question 19

mycobacterium produced red blank and is blank because it was not affected by decolorizer

Answer 19

rods, acid fast

Question 20

S. aureus produced blue blank and is blank because it was affected by decolorizer

Answer 20

cocci, not acid fast

Question 21

differential and structural stain can be a blank stain

Answer 21

spore

Question 22

only gram blank can form spores (blank genera)

Answer 22

positive, 5

Question 23

the nasty death bacteria that smells awful and forms spores and is tiny

Answer 23

C. sporogens

Question 24

also forms spores and is larger than C. sporogens

Answer 24

B. cereus

Question 25

why does spore stains need to be steamed

Answer 25

soften spore coat to absorb dye

Question 26

spores are blank color

Answer 26

green

Question 27

vegetative cells are blank color

Answer 27

red

Question 28

first culture we made

Answer 28

S. marcescens

Question 29

1 type of organism in culture

Answer 29

pure

Question 30

streak technique we used

Answer 30

t stain

Question 31

when t staining areas 2 and 3, you should go back into the previous quadrant to grab more organisms blank and start in the previous blank

Answer 31

once, quadrant

Question 32

tuberculosis bacteria

Answer 32

mycobacterium

Question 33

fuchsia stain is also called blank

Answer 33

carbol

Question 34

example of non spore former

Answer 34

E. coli

Question 35

four gram stain steps

Answer 35

1. crystal violet 2. iodine mordant 3. decolorization 4. saffranin

Question 36

7 acid fast steps

Answer 36

1. heat fix 2. steam and put carbol fuchsian stain on 3. rinse with water 4. rinse with decolorizer 5. rinse with water 6. methylene blue 7. rinse with water

Question 37

5 spore stain steps

Answer 37

1. heat fix 2. malachite green then steam 3. rinse with water (decolorizer) 4. safranin 5. rinse with water