Lab quiz 3

Question 1

sterile

Answer 1

The killing or removal of all microorganisms on an object or in a material. This includes spores

Question 2

Disinfectant

Answer 2

A chemical agent that is used on inanimate objects to destroy microorganisms
-few are capable of killing spores
-only used on surfaces (toxic)

Question 3

Antiseptic

Answer 3

A chemical agent that is used in association with the skin and mucous membranes to destroy microorganisms or inhibit their growth
-used for humans

Question 4

Antibiotic

Answer 4

chemical used to treat infections
-can be used on surfaced and taken as meds due to low toxicity
-natural, semisynthetic, and synthetic chemicals

Question 5

-cidal

Answer 5

an agent that has killing properties (not typically with spores)

Question 6

-static

Answer 6

an agent that has growth-controlling properties (inhibits, but does not kill)

Question 7

broad spectrum

Answer 7

an agent that exerts an effect against many different organisms

Question 8

narrow spectrum

Answer 8

an agent that exerts an effect against only a few organisms

Question 9

What are the examples of disinfectants

Answer 9

bleach, formaldehyde, lysol and pinesol, and ammonia

Question 10

What are examples of antiseptics

Answer 10

70% isopropyl alcohol, 3% peroxide, mouthwash, and betadine

Question 11

What are the limitations of the filter paper disk method?

Answer 11

human errors
chemical properties influencing the diffusion onto the agar (molecular weight, hydrophobicity, and volatility)

Question 12

Molecular weight

Answer 12

the larger the weight the longer it takes to diffuse
the smaller the weight the less time it takes to diffuse

Question 13

hydrophobicity

Answer 13

How much the chemical can permeate water
-agar plate is mainly water so hydrophilic will be better off

Question 14

Volatility

Answer 14

How easily a chemical evaporates

Question 15

What media is used for the Kirby-Bauer method?

Answer 15

MHA

Question 16

Why do we have to compare the zone of inhibition to a standard table to determine the sensitivity of an organism?

Answer 16

Because ADME does not occur on an agar plate, but it does occur in the body

Question 17

ADME

Answer 17

Absorption, metabolism, distribution, and elimination

Question 18

Morphology

Answer 18

the shape of ONE individual cell

Question 19

Arrangement

Answer 19

The pattern of cells as they grow in culture

Question 20

Spherical bacteria

Answer 20

Cocci

Question 21

Rod-shaped bacteria

Answer 21

bacilli

Question 22

Diplo

Answer 22

Cocci in pairs
bacilli end-to-end in pairs

Question 23

Tetrad

Answer 23

cocci in squares w 4

Question 24

strepto

Answer 24

cocci chain (end to end)
bacilli chain (end to end)

Question 25

staphylo

Answer 25

cocci in random formation

Question 26

single

Answer 26

cocci by themselves bacilli by themselves -endospores show up clear (only found in G+)

Question 27

Decolorization

Answer 27

The differential gram staining step

Question 28

G+ bacilli may stain G- if it is an older sample

Answer 28

ex. B. meg