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Laboratory Safety - part 1 > Lab Safety Part One > Flashcards

Lab Safety Part One Flashcards

1
Q

List 5 sharp objects

A

Needles, lancets, broken glass, microtome, blades

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2
Q

What main things can cause a fire?

A 
Unauthorized smoking
Electrical issues
Open flames
Explosive chemicals
Static electrical charges
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3
Q

What are the 4 general fire-fighting tools?

A

Sprinkler system
Fire hoses
Fire blankets
Fire extinguishers

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4
Q

List the 3 classes of fire extinguishers, their color, and what they’re used for

A

Class A - green - wood and paper
Class B - red - grease, gasoline, oil
Class C - blue - liquids and gases

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5
Q

What three things does fire require to burn?

A

Heat, oxygen, fuel

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6
Q

What does PASS stand for?

A

P - ull (the locking pin out)
A - im (the nozzle at base of flame)
S - queeze (operating handle)
S - weep (motion that covers entire burning surface)

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7
Q

What does REACT stand for?

A 
R - remove patients and alert other staff
E - ensure all doors are closed
A - activate fire alarm
C - call fire department
T - try to extinguish fire
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8
Q

How often should fire extinguishers be recharged?

A

Yearly

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9
Q

What is a corrosive chemical?

A

strong acids or bases that injure body tissues and corrode metal

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10
Q

What is a caustic chemical?

A

by contact, they can burn, eat away or destroy tissue

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11
Q

Poisonous chemical?

A

Can cause immediate death if ingested

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12
Q

Carcinogenic chemical?

A

known to cause cancer to humans or lab animals

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13
Q

Mutagenic chemical?

A

causes genetic mutations

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14
Q

Reactive and volatile chemicals?

A

when mixed with other chemicals can cause bad reactions and explosions

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15
Q

Flammable and combustible?

A

can catch fire easily

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16
Q

What is a laminar flow hood used for?

A

used when working with carcinogenic and mutagenic materials

17
Q

What are the 5 things laminar flow hoods or bio safety cabinets do?

A

1) Protects the employee
2) Protects the specimen
3) Protects the lab environment
4) Has HEPA filter to absorb and filter contaminants
5) Has a barrier in front of the worker

18
Q

When is a fume hood used?

A

it’s used when working with strong acids and bases

19
Q

True or false: you should never store glacial acetic acid in the fridge?

A

True

20
Q

Where are all acids stored?

A

in glass containers

21
Q

Where are all bases stored?

A

in plastic containers

22
Q

What is the main use of phenol?

A

used as disinfectant

23
Q

What is iodine most suitable for?

A

destroying HIV and HBV

24
Q

What is chlorhexidine?

A

Alternative to iodine

25
Q

Complete the sentence: Chemicals that are highly flammable are…

A

organic solvents

26
Q

What should you always add the acids and bases to when preparing solutions?

A

Water

27
Q

Where can we find mercury?

A

Thermometers and stationary blood pressure equipment

28
Q

How to handle dangerous chemicals?

A

Pour chemicals below eye level
Add acid and alkalis to water when mixing
Use bottle carriers for larger bottles when transporting

29
Q

What is lethal dose 50% What is another way it’s written?

A

given orally once or through IV to test animals, kills half in number
LD.50

30
Q

What is lethal concentration 50%

A

the concentration of a toxic substance inhaled by test animals, kills 50%
LC.50

31
Q

What does OPIM stand for?

A

Other Potential Infectious Materials

32
Q

List 5 fundamental requirements

A

1) No eating or drinking in the lab
2) Tie back long hair
3) Wear PPE
4) Keep exits, passageways, and access to safety equipment free from obstruction
5) Keep unauthorized people out of the lab

33
Q

What are 5 Safety Station Equipment?

A

first aid book, PPE, flashlight, eye-wash station, fire extinguisher

34
Q

What are UP (universal precautions)?

A

the concept that all blood and body fluids should be treated as infectious

35
Q

What are standard precautions?

A

infectious status of patients applies to:

blood, all body fluids except sweat, non-intact skin, mucous membrane

36
Q

What are biological hazards?

A

bacteria, fungi, viruses, feces

Laboratory Safety - part 1 (3 decks)

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