Lab Techniques

Question 1

Gas Chromatography

Answer 1

Mobile Phase:Inert Gas/carrier gas
Stationary Phase:Liquid/Solid
Elute First: Gas with low boiling point or smaller molecule if boiling points are similar if the gas has a high boiling point

Question 2

Thin Layer Chromatography

Answer 2

Mobile: Liquid ex; DCM
Stationary: Solid ex;silica
Elute first: compound most similar to liquid phase

Question 3

Column Chromatography

Answer 3

Mobile: Solvent
Stationary:silica/alumina
Elute first: samples similar to solvent
Depends on: size of compound and similarity to solvent

Question 4

Ion Exchange Chromatography

Answer 4

Mobile:
Stationary: charged
Elute First:same charge, opposite charge are stuck to column

Question 5

Size-Exclusion Chromatography

Answer 5

Mobile:
Stationary: Beads with pores
Elute: Depends on size, usually to catch tiny molecules and let pass the larger ones

Question 6

Affinity Chromatography

Answer 6

Stationary: coated with receptors for particular protein