LABORATORY ACTIVITY 9

Question 1

 RBC is (normocytic)

Answer 1

6-8 mm

Question 2

 Lower than 6 =

Answer 2

microcytic

Question 3

 Higher than 8=

Answer 3

macrocytic

Question 4

 Normal shape:

Answer 4

biconcave discoid

Question 5

 If there are spikes it can be:

Answer 5

acanthocytes or spur cells

Question 6

 Normal color of RBC:

Answer 6

Pink to orage or salmon pink

Question 7

 Normal color of Reticulocyte:

Answer 7

Pinkish gray or blue-gray

Question 8

INCLUSIONS ● We can see

Answer 8

malarial parasites, basophilic stippling, howell jolly bodies, heinz bodies

Question 9

PURPOSE:
1. Serve as a [?] to verify the result produced by automated analyzers.
2. Shows generalized B, problem that leads to [?]

Answer 9

quality control

anemia

Question 10

○ Ex. you are wondering why RBC is very low so you prepared a smear and you saw that there are acanthocytes, spherocytes and other abnormal cells, so we do this to verify and to compare the results with that of the [?]

Answer 10

automation

Question 11

○ Remember the principle of automation is [?]. So it detects using the size of the RBCs. If RBC is smaller or bigger than normal, it will not count those RBCs. Leading to low RBC count.

Answer 11

electrical impedance and light scattering

Question 12

o Especially if there are problems in WBC and Platelets. Why? For example, we have hypersegmented WBC, so maybe it is not only a problem with the WBC but also in the RBC like [?].

Answer 12

anemia

Question 13

o Hypersegmented WBC also exist in [?] and both of these deficiencies can cause anemia. So maybe there is a problem with the erythropoiesis of the bone marrow.

Answer 13

Vit. B12 deficiency and folate deficiency

Question 14

o [?] are important for the maturation of RBC in the BM.

Answer 14

Folate and Vit. B12

Question 15

o If there is decreased platelet, it is

Answer 15

acute ammonia

Question 16

PREPARATION OF WEDGE SMEAR
● [?] drop of blood
● [?] angle for rodaks and (25-40) angle for steninger

Answer 16

2-3 mm

30-45

Question 17

QUALITY
● Film is [?] of the slide
● [?], buller form is not allowed
● [?] edge (no dropping marks)
● Without [?]
● [?] when exposed to light
● In stain blood cell examination, we are going to read until the [?] of the stained sample.

Answer 17

⅔ to ¾

Finger shaped/ tongue shaped

Feathery

holes/irregularities

Rainbow

center

Question 18

FACTORS AFFECTING A GOOD SMEAR

Answer 18

(P) Pressure

(A) Angle

(S) Size

(S) Speed

Question 19

(P) Pressure

Thick:
Thin:

Answer 19

Decrease
Increase

Question 20

(A) Angle

Thick:
Thin:

Answer 20

Increase
Decrease

Question 21

(S) Size

Thick:
Thin:

Question 22

(S) Speed

Thick:
Thin:

Question 23

● Romanowsky stain (?) : primary or contrasting stain

Answer 23

Wright stain/ Wright Giemsa

Question 24

● [?] (has many colors)

Answer 24

Polychrome stain

Question 25

● stains RNA (acidic): [?]

Answer 25

Free methylene blue

Question 26

● stains Hemoglobin (basic) and eosinophilic granules (basic): [?]

Answer 26

Free eosin or eosin y

Question 27

which is negatively charged and acidic

Answer 27

Free eosin or eosin y

Question 28

which is positively charged and basic

Answer 28

Free methylene blue

Question 29

Thick films

Answer 29

EXCESSIVE BLUE STAIN

Question 30

Insufficient staining time

Answer 30

EXCESSIVE PINK

Question 31

Prolonged staining time

Answer 31

EXCESSIVE BLUE STAIN

Question 32

Prolonged washing time

Answer 32

EXCESSIVE PINK

Question 33

Inadequate washing

Answer 33

EXCESSIVE BLUE STAIN

Question 34

High acidity of stain

Answer 34

EXCESSIVE PINK

Question 35

Too high alkalinity of buffer

Answer 35

EXCESSIVE BLUE STAIN

Question 36

Mounting coverslip before the slide is dry

Answer 36

EXCESSIVE PINK

Question 37

You only mount a coverslip when you are going to preserve

Answer 37

EXCESSIVE PINK

Question 38

 side to side

Answer 38

1. CROSS SECTIONAL

Question 39

 tail towards the head

Answer 39

2. LONGITUDINAL METHOD

Question 40

 reference and the preferred method, so first vertically then horizontally

Answer 40

3. BATTLEMENT METHOD

Question 41

 we are going to count three consecutive horizontal edge fields, so two fields is towards the center and two fields horizontally and two fields vertically

Answer 41

4. HORIZONTAL EDGE

Question 42

Abnormal size =

Answer 42

Anisocyte

Question 43

Abnormal shape =

Answer 43

Poikilocyte

Question 44

Normal color of RBC: Salmon pink (?)

Answer 44

normal Hb synthesis

Question 45

● Decreased color w/ increased central pallor =

Answer 45

Hypochromic

Question 46

● Increased color w/ decreased central pallor =

Answer 46

Hyperchromic

Question 47

● Normal: central pallor

Answer 47

1⁄3

Question 48

(failure to degrade RNA in the retic due to deficiency in Pyrimidine 59 nucleotidase

Answer 48

basophilic stippling

Question 49

Distance from the edge of the slide

Answer 49

1 cm

Question 50

Wright Giemsa (?)

Answer 50

Carbol Fuschin and Methylene Blue

Question 51

a polychrome stain: has two colors staining [?]

Answer 51

cytoplasm and nucleus

Question 52

Laboratory Activity 9: STAINED BLOOD CELL EXAMINATION Specimen Required:

Answer 52

Stained blood smears (CSR Control slides)

Question 53

STAINED BLOOD CELL EXAMINATION 1. Using oil immersion objective, study a thin area of the blood smear. Cells must be (?). 2. Examine about (?) microscopic fields and make observations of: (?) 3. Report the degree of anisocytosis and/or poikilocytosis following the standard manner of reporting as (?)

Answer 53

evenly distributed and free of stain precipitates, not overlapping nor bunched together 15 a. RBCs’ hemoglobin content, size, shape, and presence of inclusion bodies. b. WBCs’ cytoplasm and nucleus c. Platelets’ size, shape and disrtibution - ; +1, +2, +3 or +4