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BACT 211 > Laboratory Diagnosis of Enterobacteriaceae > Flashcards

Laboratory Diagnosis of Enterobacteriaceae Flashcards

(72 cards)

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1
Q

Yersinia spp. bipolar appearance, closed safety pin appearance.

A

Wayson stain or Methylene Blue stain

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2
Q

Blue rod with prominent polar granules, giving rise to the safety-pin appearance,surrounded by a large, pink capsule.

[microscopic examination]

A

Donovan Body

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3
Q

Used as enrichment for the isolation of Shigella and other Enterobacteriaceae.

[culture media]

A

Gram-negative broth (GN) or Selenite broth (SEL)

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4
Q

Medium specifically used for the isolation of Y. enterocolitica from gastrointestinal specimens.

[culture media]

A

Cefsulodin-irgasan-novobiocin (CIN) agar

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5
Q

It is used to differentiate sorbitolnegative E. coli O157:H7 from other strains of E. coli that are capable of fermenting this sugar alcohol.

[culture media]

A

MacConkeysorbitol agar (MAC-SOR)

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6
Q

Broth cultures of Y. pestis exhibit a characteristic ____ in which clumps of cells adhere to one side of the tube.

A

Stalactite pattern

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7
Q

Colonies of Y. pestis on 5% sheep blood agar are ____ at 24 hours but exhibit a ___, ____ appearance at 48 hours.

A

pinpoint, rough, cauliflower

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8
Q

Production of hydrogen sulfide (H2S); ferrous sulfate. Salmonella spp. Positive reactions appear as brown to black precipitate.

[isolation of salmonella spp. and shigella spp.]

A

Bismuth sulfite agar

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9
Q

Positive reactions of Bimuth Sulfite Agar.

[color]

A

brown to black

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10
Q

Salmonella spp. appear as white to pink or red colonies surrounded by a bright halo.

[isolation of salmonella spp. and shigella spp.]

A

Brilliant green agar

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11
Q

Appear colorless.

[shigella, salmonella]

A

Shigella spp.

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12
Q

Colorless with a black center.

[shigella, salmonella]

A

Salmonella spp.

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13
Q

Selective enrichment for the growth of Salmonella spp.

A

Selenite broth

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14
Q

Colorless to red with black center. (Xylose-lysinedeoxychocolate agar)

[shigella, salmonella]

A

Salmonella spp.

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15
Q

Colorless to red. (Xylose-lysinedeoxychocolate agar)

[shigella, salmonella]

A

Shigella spp.

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16
Q

Lactose fermenter, forms blueblack with a metallic green sheen.

[Eosin/Methylene blue agar (EMB]

A

Escherichia coli

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17
Q

form pink colonies.

[Eosin/Methylene blue agar (EMB]

A

Other coliform fermenters

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18
Q

Translucent, either amber or colorless.

[Eosin/Methylene blue agar (EMB]

A

Nonfermenters

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19
Q

TSI means

A

Triple Sugar Iron agar

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20
Q

[3] Sugar in TSI agar

A

Glucose
Lactose
Sucrose

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21
Q

acidic (yellow) resulting from the fermentation of glucose under anaerobic conditions.

[TSI]

A

Butt

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22
Q

lactose and/or sucrose are fermented:

[TSI]

A

Siant

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23
Q

[3] Quality Control

A
  1. A/A
  2. K/A H2S+
  3. K/NC
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24
Q

A/A

[quality control]

A

Escherichia coli

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25
K/A H2S+ [quality control]
Salmonella typhi
26
K/NC: [quality control]
Pseudomonas aeruginosa
27
Drawing a circle around the A for the acid butt, that is, A/A , usually indicates production of ____?
Gas
28
Indicate the production of CO2 and H2 in the tube.
Bubbles or cracks
29
Contains lysine, peptones, a small amount of glucose, ferric ammonium citrate, and sodium thiosulfate.
Lysin Iron Agar
30
When glucose is fermented, the butt of the medium becomes ___?
Acidic (yellow)
31
What forms if the organism produces lysine decarboxylase?
Cadaverine
32
It neutralizes the organic acids formed by glucose fermentation, and the butt of the medium reverts to the alkaline state (purple)
Cadaverine
33
Organism decarboxylates but cannot deaminate, ferments glucose, first butt is yellow then turns back to purple. [LIA]
K/K
34
Organism fermented glucose but was unable to deaminate or decarboxylate lysine. [LIA]
K/A
35
Organism deaminated lysine but could not decarboxylate it. [LIA]
R/A
36
Lysine decarboxylation and no fermentation of glucose. [LIA result]
Alkaline slant/ Alkaline butt (K/K)
37
Glucose fermentation [LIA result]
Alkaline slant/acid butt (K/A)
38
Lysine deamination and glucose fermentation. [LIA result]
Red slant/acid butt (R/A)
39
Alkaline slant and butt, H2S positive: [LIA, quality control]
Salmonella typhimurium
40
Alkaline slant, acid butt: [LIA, quality control]
Shigella flexneri
41
Red slant, acid butt: [LIA, quality control]
Proteus vulgaris
42
Determines whether an organism possesses the enzyme that deaminates phenylalanine to phenylpyruvic acid. [test]
Phenylalanine Deaminase (PAD)
43
This test is helpful in initial differentiation of Proteus, Morganella, and Providencia organisms, which are positive, from the rest of the Enterobacteriaceae.
Phenylalanine Deaminase (PAD)
44
A semisolid agar.
Sulfide indole motility
45
Color that indicates the production of H2S. [SIM agar]
Black precipitate
46
Color that indicated the addition of Kovac's reagent is positive for indole. [SIM agar]
pink to red
47
It is one of the degradation products of the amino acid tryptophan [SIM agar]
Indole
48
What color that indicates that indole is present. [SIM agar]
Red
49
In SIM agar medium, it can be utilized by bacteria, with the production of hydrogen sulfide.
Sodium thiosulfate
50
What color is H2S in SIM agar.
Colorless
51
It is more sensitive than Kovac’s reagent and is preferred with nonfermentative bacteria. [method]
Ehrlich method
52
Color that indicate that indole is present after the addition of PDAB.
Red color
53
This test is used to determine the ability of an organism to utilize sodium citrate as its only carbon source and inorganic ammonium salts as its only nitrogen source. [test]
Citrate utilization
54
Color of bacteria that can grown on citrate utilization.
Bromothymol blue
55
Bromothymol Blue is GREEN. [pH]
pH <7.0
56
Bromothymol BLUE [pH]
pH > 7.6
57
Quality control: Positive [Citrate utilization]
Klebsiella pneumoniae
58
Quality control: Negative [Citrate utilization]
Escherichia coli
59
Alternative medium of Citrate Utilization.
Christensen's citrate
60
This medium incorporates phenol red (as the pH indicator) and organic nitrogen.
Christensen's citrate medium
61
This test is used to determine the ability of an organism to produce the enzyme urease, which hydrolyzes urea.
Urea hydrolysis (Christensen's method)
62
[2] Hydrolysis of urea produces. [A,C]
Ammonia and CO2
63
Expected results of Urea Hydrolysis if NEGATIVE.
No color change
64
Expected results of Urea Hydrolysis if POSITIVE.
Light orange to magenta
65
Quality control of Urea Hydrolysis: POSITIVE
Proteus vulgaris
66
Quality control of Urea Hydrolysis: NEGATIVE
Escherichia coli
67
Quality control of Methyl Red: POSITIVE
Escherichia coli
68
Quality control of Methyl Red: NEGATIVE
Enterobacter cloacae
69
Quality control of B. VP (Barritt's method): POSITIVE
Enterobacter cloacae
70
Quality control of B. VP (Barritt's method): NEGATIVE
Escherichia coli
71
This test determines whether an organism has the ability to reduce nitrate to nitrite and reduce nitrite further to nitrogen gas (N2).
Nitrate Reduction Test
72
Color that indicates of presence of nitrite.
Red

BACT 211 (20 decks)

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