Laboratory Equipment and Supplies

Question 1

Type of glass that tolerates heat, sterilization but has low chemical resistance.

Answer 1

Borosilicate (Pyrex, Kimax)

Question 2

Glass that is 6x stronger than borosilicate and resists scratches and alkali attack.

Answer 2

Aluminosilicate (Corex)

Question 3

Glass with excellent heat, chemical, and electrical tolerance, used for drastic heat shock and acid/alkali treatment.

Answer 3

High Silica (Vycor)

Question 4

Glass that melts when heated, is boron-free, and has high chemical resistance.

Answer 4

Soft glass

Question 5

Most inexpensive glass that releases alkali, causing errors in tests.

Answer 5

Flint glass (soda lime glass)

Question 6

Amber-colored glass used to reduce light transmission for photosensitive substances.

Answer 6

Low actinic glass

Question 7

Plastic materials that are autoclavable.

Answer 7

Teflon, Polypropylene, Polycarbonate

Question 8

Plastic with excellent temperature and chemical resistance.

Answer 8

Teflon

Question 9

Plastic resistant to chemicals, tolerates heat, and is used for pipet tips and cryogenic storage.

Answer 9

Polypropylene

Question 10

Plastic stronger than polypropylene but with lower chemical resistance, used for centrifuge tubes.

Answer 10

Polycarbonate

Question 11

Non-autoclavable plastic materials.

Answer 11

Polyethylene, Polystyrene, Polyvinyl chloride

Question 12

Plastic resistant to most chemicals except concentrated acids, used for alkaline solution storage.

Answer 12

Polyethylene

Question 13

Rigid, clear plastic used for test tubes and graduated tubes.

Answer 13

Polystyrene

Question 14

Soft, flexible, porous plastic often used as tubing.

Answer 14

Polyvinyl chloride

Question 15

Pipette design that holds but does not deliver the exact volume.

Answer 15

To Contain (TC)

Question 16

Pipette design that delivers the exact volume it holds.

Answer 16

To Deliver (TD)

Question 17

Pipette with an etched ring or band near the mouth, requiring blow-out.

Answer 17

Blow-out pipette

Question 18

Pipettes that drain by gravity.

Answer 18

Self-draining pipettes

Question 19

Examples of pipettes designed to contain (TC).

Answer 19

Sahli pipette, Lang-Levy, Glass micropipettes

Question 20

Pipette with a cylindrical bulb, single capacity, and self-draining for nonviscous samples.

Answer 20

Volumetric pipette

Question 21

Pipette with an oval bulb, calibrated to contain (C.M) 1 mL, used for viscous fluids.

Answer 21

Ostwald-Folin pipette

Question 22

Pipette graduated to the tip and requiring blow-out.

Answer 22

Serologic pipette

Question 23

Pipette with point-to-point delivery, self-draining, and requires controlled drainage.

Answer 23

Mohr pipette

Question 24

Uses of measuring/graduated pipettes.

Answer 24

Serial dilution; measuring reagents

Question 25

Types of semi-automatic micropipettors.

Answer 25

Air displacement, Positive displacement

Question 26

Micropipettor that uses suction with a disposable polypropylene tip and no piston contact with liquid.

Answer 26

Air displacement micropipettor

Question 27

Micropipettor that operates like a syringe, with no air cushion and constant aspiration for viscous/high-density samples.

Answer 27

Positive displacement micropipettor

Question 28

Components of an air displacement pipettor.

Answer 28

Piston with air cushion; disposable polypropylene tip

Question 29

Components of a positive displacement pipettor.

Answer 29

Disposable piston, disposable capillary, disposable seal

Question 30

Application of positive displacement micropipettor.

Answer 30

Discrete automated systems for viscous/high-density samples

Question 31

Calibration frequency

Answer 31

Every 6 months

Question 32

Gravimetric method

Answer 32

Weight of distilled water (d=1g/mL, 20°C) using analytical balance for accuracy

Question 33

Spectrophotometric method

Answer 33

Absorbance of colored solution (K dichromate or p-nitrophenol) delivered

Question 34

Horizontal or swinging bucket centrifuge

Answer 34

Max speed 3000 RPM, tubes horizontal during spinning, good for decantation

Question 35

Fixed-angle centrifuge

Answer 35

Tubes at fixed angle, capable of up to 7000 RPM, less heat build-up

Question 36

Ultracentrifuge

Answer 36

Up to 100,000 RPM, refrigerated for lipoprotein separation, gold standard

Question 37

Cytocentrifuge

Answer 37

Used for body fluid cell counts, speed range 200-2000 RPM

Question 38

Units used for centrifuge speed

Answer 38

RPM, RCF (g), Svedberg (S)

Question 39

Formula to convert rpm to RCF

Answer 39

1.118 x 10^-5 x r (cm) x RPM

Question 40

Centrifuge QC parameters

Answer 40

Timer, speed check, refrigerated centrifuge temp check using tachometer

Question 41

Centrifuge calibration frequency

Answer 41

Every 3 months

Question 42

Analytic Reagent Grade (ACS) chemicals

Answer 42

High purity suitable for most analytical procedures

Question 43

Ultrapure Grades chemicals

Answer 43

Used for chromatography, AAS, immunoassays, molecular diagnostics

Question 44

Chemically Pure Grade chemicals

Answer 44

Impurity limitations not stated, not suitable for research without further purification

Question 45

USP/NF Grade chemicals

Answer 45

Used for drug manufacture, purity may not meet assay requirements

Question 46

Technical/Commercial Grade chemicals

Answer 46

Not suitable for clinical laboratory use

Question 47

Primary standard

Answer 47

Highly purified chemical with known concentration and purity

Question 48

Secondary standard

Answer 48

Lower purity, concentration determined by comparison with primary standard or SRM

Question 49

Max colony count (CFU/mL) Type I

Answer 49

<10

Question 50

Max colony count (CFU/mL) Type II

Answer 50

<1000

Question 51

Max colony count (CFU/mL) Type III

Answer 51

Not specified

Question 52

pH of Type III

Answer 52

5.0 to 8.0

Question 53

Silicate (mg/L SiO2) Type I

Answer 53

0.05

Question 54

Silicate (mg/L SiO2) Type II

Answer 54

0.1

Question 55

Silicate (mg/L SiO2) Type III

Answer 55

1