LABORATORY SAFETY AND INFECTION CONTROL

Question 1

used for dangerous chemicals

Answer 1

Chemical fume hood

Question 2

protect the users

Answer 2

chemical fume hood

Question 3

no hepa filter

Answer 3

chemical fume hood

Question 4

exhaust air outside the building

Answer 4

chemical fume hood

Question 5

used for infectious biological agents, protects the user, the environment and the material

Answer 5

Biosafety cabinet

Question 6

possess HEPA filter does not exhaust air outside the building without decontamination

Answer 6

Biosafety cabinet

Question 7

HEPA

Answer 7

HIGH EFFICIENCY PARTICULATE AIR

Question 8

A device that encloses a working area
to protect workers from aerosol
exposure and infectious disease
agents.

Answer 8

Biosafety cabinet

Question 9

the air that contains the
infectious materials is sterilized, either
by heat, UV light or passage through a
high-efficiency particulate (HEPA)
resistance filter.

Answer 9

BSC

Question 10

Open-fronted type of cabinet with
negative pressure (ventilated
cabinets)

Answer 10

CLASS I CABINET

Question 11

It allows room (unsterilized) air to
enter the cabinet, circulate around
the area and expose the material
within; only the air to be exhausted
is sterilized using a HEPA filter.

Answer 11

CLASS I CABINET

Question 12

It is used for biosafety levels 2 and 3
agents

Answer 12

CLASS I CABINET

Question 13

It is also known as the laminar flow BSC

Answer 13

CLASS II CABINET

Question 14

The most commonly used BSC in a clinical
microbiology laboratory.

Answer 14

Class IIA

Question 15

Sterilizes the air using HEPA filter that flows over the infectious material and the air to be exhausted.

Answer 15

CLASS II CABINET

Question 16

has fixed opening; 70% of the air is recirculated

Answer 16

Class IIA

Question 17

variable sash opening; used for chemicals, radioisotopes and carcinogens.

Answer 17

Class IIB

Question 18

Provides the highest level of
safety to the worker

Answer 18

CLASS III CABINET

Question 19

The air coming into and going out
of the cabinet is sterilized using
HEPA filter and the infectious
material within is handled with
rubber gloves that are attached
and sealed in the cabinet.

Answer 19

CLASS III CABINET

Question 20

It is used for BSL 4 agents.

Answer 20

CLASS III CABINET

Question 21

Saccharomyces cerevisiae, E. coli K-12, and non-infectious bacteria

Answer 21

BSL-1

Question 22

Hepatitis a virus, Streptococcus pyogenes, Borrelia burgdorferi (Lyme disease), Salmonella species

Answer 22

BSL-2

Question 23

Yersinia pestis (plague), Mycobacterium tuberculosis, SARS, rabies virus, West Nile Virus, hanta viruses

Answer 23

BSL-3

Question 24

Ebola virus, smallpox virus

Answer 24

BSL-4

Question 25

Not known to consistently cause disease in healthy adult humans, and of minimal potential hazard to laboratory personnel and the environment.

Answer 25

BSL-1

Question 26

Moderate potential hazard to personnel and the environment. Includes bacteria and viruses that cause mild disease to humans, or are difficult to contract via aerosol in a lab setting.

Answer 26

BSL-2

Question 27

Microbes there can either indigenous or exotic, and they can cause serious or potentially lethal disease through respiratory transmission.

Answer 27

BSL-3

Question 28

Dangerous and exotic, posing a high risk of aerosol-transmitted infections. Infections caused by these microbes are frequently fatal and without treatment or vaccines.

Answer 28

BSL-4

Question 28

- No Containment - Defined organisms -Unlikely to cause disease

Answer 28

BSL-1

Question 29

- Containment - Moderate Risk - Disease of varying severity

Answer 29

BSL-2

Question 30

- High Containment - Aerosol Transmission - Serious/ Potentially lethal disease

Answer 30

BSL-3

Question 31

- Max Containment - "Exotic", High- Risk Agents - Life- threatening disease

Answer 31

BSL-4

Question 32

E. coli

Answer 32

BSL-1

Question 33

Influenza, HIV, Lyme Disease

Answer 33

BSL-2

Question 34

Tuberculosis

Answer 34

BSL-3

Question 35

Ebola Virus

Answer 35

BSL-4

Question 36

Agents that present minimal potential hazard to personnel & the environment.

Answer 36

BSL-1

Question 37

Agents associated with human disease & pose moderate hazards to personnel & the environment.

Answer 37

BSL-2

Question 38

Indigenous or exotic agents, agents that present a potential for aerosol transmission, & agents causing serious or potentially lethal disease.

Answer 38

BSL-3

Question 39

Dangerous & exotic agents that pose a high risk of aerosol-transmitted laboratory infections & life-threatening disease.

Answer 39

BSL-4

Question 40

Pass-thru autoclave with Bioseal required in laboratory room.

Answer 40

BSL-3

Question 41

Pass-thru autoclave with Bioseal required in laboratory room.

Answer 41

BSl-4

Question 42

These are agents that pose the greatest public health threat.

Answer 42

Category A Agents

Question 43

They are easily transmitted and highly infectious.

Answer 43

Category A Agents

Question 44

Smallpox, Bacillus anthracis, and Francisella tularensis

Answer 44

Category A Agents

Question 45

These are agents with moderate morbidity and low mortality.

Answer 45

Category B Agents

Question 46

Not easily transmitted as category A agents.

Answer 46

Category B Agents

Question 47

Coxiella burnetti, Burkholderia pseudomallei, and Rickettsia

Answer 47

Category B Agents

Question 48

These are the emerging pathogens

Answer 48

Category C Agents

Question 49

Viruses that causes yellow fever, dengue, influenza and SARS

Answer 49

Category C Agents

Question 50

1. Hand Hygiene 2. Gown 3. Respiratory Protection 4. Eye Protection 5. Gloves

Answer 50

Donning Your PPE

Question 51

1. Gloves 2. Eye Protection 3. Gown 4. Respiratory 5.Hand Hygiene.

Answer 51

Doffing your PPE

Question 52

Substances which singly or in combination have a significant threat or hazard to human health or to the environment and require special handling.

Answer 52

Hazardous Wastes

Question 53

Should be calibrated against a reference thermometer from the National Institute of Standards and Technology (NIST).

Answer 53

Thermometer Calibration

Question 54

Thermometer that differ by ___ from the reference thermometer should be disposed.

Answer 54

>1degree C

Question 55

Thermometer should be checked ___ for the presence of gas bubbles.

Answer 55

daily

Question 56

Immersed in glycerol. (Prevents fluctuations when door is opened)

Answer 56

Thermometer Calibration

Question 57

The temperature used for calibration are:

Answer 57

-20 °C, 2°C to 8°C, 37 °C, and 56°C

Question 58

The percentage of carbon dioxide must be checked ____.

Answer 58

Daily

Question 59

The speed or revolution per minute (rpm) must be checked _____ using a tachometer.

Answer 59

Checked twice a year

Question 60

culture media can be checked based on their performance and sterility., and

Answer 60

records should be kept for at least two years.

Question 61

Media that are not quality-controlled by the laboratory must still undergo observation for

Answer 61

moisture, sterility, breakage and appearance.

Question 62

reagent should be tested daily with both positive and negative controls.

Answer 62

daily

Question 63

Antimicrobial Susceptibility uses specific strains of microorganisms from ____.

Answer 63

American Type Culture Collection (ATCC)

Question 64

Antimicrobial solutions must ______ after thawing.

Answer 64

not be frozen

Question 65

A stock culture should be grown in large volume of broth and then divided into _____ to last for a year.

Answer 65

vials

Question 66

To maintain viability: it should be ____ after thawing.

Answer 66

Sub- Cultured twice

Question 67

7. Stock Cultured Organisms stored in freezer should be kept at ____

Answer 67

-70 degrees C

Question 68

All tests performed on patients must be subjected to proficiency testing

Answer 68

Twice a year