Laboratory Safety Guidelines

Question 1

Before the actual experiment, what must you do?

Answer 1

Prepare a work plan

Question 2

What does a work plan contain?

Answer 2

(familiarize) objectives, instructions and procedures

Question 3

What must you wear to ensure personal safety in the laboratory?

Answer 3

Prescribed Personnel Protective Equipment

Question 4

What must be worn during experiments?

Answer 4

Safety goggles
Closed shoes (at all times)
Prescribed Laboratory Gown
Protective Face mask (recommended)

Question 5

As an additional safety measure, ________ should be properly secured to avoid interference in your work.

Answer 5

long hair and loose clothing

Question 6

What should you immediately report to your laboratory instructor?

Answer 6

any major spills, breakages, accidents
all physical injuries sustained in the lab (no matter how trivial)
all “near-miss” incidents

Question 7

The students must wear PPE to enter the laboratory and carry out an experiment
What must you avoid to wear in the lab?

Answer 7

Avoid wearing jewelry

Question 8

What is strictly prohibited in the laboratory? Why?

Answer 8

Eating, drinking and chewing gum

Most chemicals in the laboratory are poisonous

Question 9

Why is smoking prohibited in the laboratory?

Answer 9

Some of the laboratory chemicals are flammable

Question 10

Flammable solvents like organic solvents must be only used under the ____________

Answer 10

fume hood

Question 11

Perform experiments only during the _________ and only under the supervision of your ____________

Answer 11

scheduled laboratory period, laboratory instructor.

Never work alone

Question 12

Only __________ experiments must be carried out during your laboratory period. Moreover, no _____________ from the experiment procedures described in this manual, is allowed without the permission of the laboratory instructor.

Answer 12

scheduled, additional experiment procedures or deviations

DO NOT PERFORM UNAUTHORIZED EXPERIMENTS.

Question 13

NEVER REMOVE CHEMICALS FROM THE LABORATORY AND STOCKROOM - why?

Answer 13

Improper handling and misuse of these chemicals could pose great health and environmental hazard.

Question 14

Do not play games or engage in horseplay in the laboratory. Concentrate on performing your experiment.

Answer 14

NO LOITERING IN THE LABORATORY

Question 15

DO NOT LEAVE AN ON-GOING EXPERIMENT SET-UP *.

Answer 15

UNATTENDED

Question 16

Carefully read the * of chemical reagent containers – check the *

Answer 16

labels, formula and the concentration.

Question 17

Examine the * and know where to find specific information on the hazards, toxicology, immediate first aid, and correct disposal of the substance listed on it

Answer 17

Material Safety Data Sheet (MSDS)

Question 18

Know the hazards associated with the chemicals as well as their physical and chemical properties.

Answer 18

HANDLE ALL CHEMICALS WITH EXTREME CAUTION.

Question 19

Most chemicals are * . Exercise caution when handling * acids and * .

Answer 19

corrosive

concentrated, ammonia

Question 20

Concentrated acids must only be used under the *

Answer 20

fume hood.

Question 21

In case of a chemical spillage, immediately take off any * clothing and wash off spilled chemicals on the skin or eyes with copious running water for at least +

Answer 21

contaminated

15 minutes.

Question 22

Take extra care in handling chemicals in the form of * . It is recommended that you wear a mask to avoid inhalation of these chemicals. If these come into contact with the eyes, wash with copious running water continuously for at least 15 minutes. Consult a physician if needed.

Answer 22

fine dust or powder

Question 23

Observe * when working in the laboratory.

Answer 23

cleanliness and orderliness

Question 24

• in the laboratory is a must

Answer 24

Cleanliness

Question 25

1. Avoid * the reagents. | 2. Practice the habit of * droppers and rods immediately after use.

Answer 25

contaminating | rinsing

Question 26

3. Refrain from accumulating dirty glassware. Use * (e.g. while heating solutions) to clean glassware. 4. Always wash or wipe off * from reagent bottles.

Answer 26

waiting times | dirt

Question 27

5. Clean up * immediately even if it is only water. | 6. * before leaving the laboratory and dry them on a clean towel.

Answer 27

spills, water | Wash your hands thoroughly

Question 28

Dispose acids and bases into *, respectively, to prevent clogging of sinks or premature corrosion of pipes.

Answer 28

acid waste container and base waste container

Question 29

Excess and waste potassium permanganate (KMnO4) should also be thrown into * containers since this is a powerful oxidant that may corrode sink pipes.

Answer 29

inorganic waste

Question 30

Excess and waste * should also be thrown into inorganic waste containers since this is a powerful oxidant that may corrode sink pipes.

Answer 30

potassium permanganate (KMnO4)

Question 31

Dispose organic wastes into *

Answer 31

halogenated organic waste containers or non-halogenated organic waste container.

Question 32

All solids used in experiment procedures (filtration, chromatography, etc.) should be disposed into *

Answer 32

contaminated solid waste containers.

Question 33

Other solid wastes should be disposed into the * available inside the laboratory.

Answer 33

trash bin

Question 34

All broken glassware including * used in experimental procedures such as melting point determination, chromatography, etc. should be disposed in *

Answer 34

capillary tubes, broken glassware container.

Question 35

What are the 4 types of waste and where should they be disposed of?

Answer 35

``` Inorganic waste (acids and bases + Potassium permanganate (KMnO4) Organic waste (non/halogenated organic waste containers Solid waste (contaminated solid waste containers; trash bin) Broken glassware (like capillary tubes - broken glassware container) ```

Question 36

Flammable organic solvents

Answer 36

ethanol, methanol, acetone

Question 37

Since there are few flammable organic solvents, a chemical fire can occur. Also, there is a small risk of electrical fire. In case a fire occurs, what is the first thing you should do?

Answer 37

``` Remain calm (Call the attention of your laboratory instructor and leave the laboratory) ```

Question 38

Fires in small containers can be extinguished by * over the burning container. Alternatively, cover the burning container with *. Never use * towels.

Answer 38

inverting a small beaker or any other similar equipment soaking wet towels dry

Question 39

Remove any flammable material such as * from the area of the fire.

Answer 39

reagent bottles with flammable chemical

Question 40

In case of a person inhaling smoke or toxic fumes, transfer him/her to a *.

Answer 40

well-ventilated area

Question 41

In case your clothing should catch on fire, use the *

Answer 41

safety shower