Lec 3-4 Flashcards

(39 cards)

1
Q

Chemiluminescence vs Fluorescence vs Phosphorescence

A

Chemiluminescence: e- excitation from chem rxn
Fluorescence: e- excitation from photon absorption
Phosphorescence: e- excitation from photon absorption, with triplet intermediate step

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2
Q

Bioluminescence vs Thermal Radiation

A

Bioluminescence: e- excitation from chem rxn of luciferin
Thermal Radiation: Light emitted

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3
Q

Evolutionary Pressure to create bioluminescence

A

Predation, evading predators, communication

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4
Q

Continuum Wave (CW) Lasers

A

Continuous wave, lower power

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5
Q

Pulsed Lasers

A

Short pulses, higher power

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6
Q

Properties of lasers

A

Coherent, monochromatic, polarized and directional

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7
Q

Applications of lasers

A

Cutting, communication, printing, scanning

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8
Q

Types of waves

A
  • Spherical Waves
  • Planar Waves
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9
Q

Huygen-Fresnel’s Principle

A

All points on a wavefront are the source of a new wave.

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10
Q

Superposition Principle

A

Because waves can interfere constructively or destructively,
the result of superpositioned waves is their sum.

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11
Q

Refraction

A

When crossing into another medium, light takes the path of shortest time, not the path of shortest distance
- (light is slower if index of refraction is high)

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12
Q

Diffraction

A

Interference or bending of waves around obstacle

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13
Q

Optical components for light manipulation

A
  • Pinholes and Irises
  • Mirrors
  • Beam Splitters
  • Filters
  • Lenses
  • Prisms
  • Gratings
  • Polarizers
  • Optic Fibers`
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14
Q

Pinholes

A
  • Control amount of light entering a system
  • Allows for formation of an image on a screen
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15
Q

Pinhole examples

A
  • Human iris
  • Pinhole eyes of some animals
  • Pinhole camera
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16
Q

Mirrors

A
  • Reflect light
  • Flat or Curved
  • Metallic or Dielectric
17
Q

Dielectric mirror

A

Are made from systems of thin lenses.

Layers of oil, water and air allow light to interfere constructively/destructively.

18
Q

Snell’s Law

A

n1sinθ_1=n2 sinθ_2

19
Q

Total internal reflection

A
  • When
    • n1 > n2
    • θ_1 > 90
  • As light θ_1 increases, more light is reflected than refracted
20
Q

Critical angle (θ_c)

A

θ_1 when θ_2 is 90°
(angle of incidence when angle of refraction is 90°)

21
Q

Filters

A

Selective for specific wavelengths

22
Q

Edge filters

A

Long Pass/Short Pass

23
Q

Long Pass Filters

A

Allow passage of long wavelengths, block short wavelengths

24
Q

Short Pass Filters

A

Allow passage of short wavelengths, block long wavelengths

25
Lens equations
1/f=1/d_i +1/d_o M = -d_i/d_o = hi/ho
26
Multiple Lens Equations
1/f = 1/f_1 + 1/f_2 M = -f_2/f_1
27
Rayleigh Length
Describes beam waist at focal point
28
Prisms
Larger refractive index for smaller wavelengths --> Different wavelengths refracted at different angles --> White light separated
29
Gratings
Harnesses wave diffraction and interference to separate white light
30
Polarizers
- Select light based orientation of electrical field - Total polarization of reflected ray achieved Brewters's angle
31
Brewster's angle (θ_B)
θ_1 when θ_2 = 90° - θ_1 (incident angle when refracted angle equal to 90 - incident angle) or (angle between refracted ray and reflected ray)
32
Brewster angle formula (derived from Snell's Law)
θ_B = arctan(n2/n1), n1 small and n2 large
33
Critical angle formula (derived from Snell's Law)
θ_c = arcsin(n2/n1), n1 large and n2 small
34
Optical fibers
- Harnesses total internal reflection to transport light - Incident angle larger or equal to critical angle
35
Optical fiber types
- Step index (aka multimode): Multiple incident angles allowed - Graded index: refractive index dependent on distance from central axis - Single mode: Single incident angle allowed to preserve signal purity
36
Examples of Light manipulation in biology
ex: Gratings in butterflies ex: Algae as lenses ex: Optical fibers in sea sponge ex: Animals with pinhole vision ex: Animals with bifocal lenses
37
Describe how can optical components be used to measure ballistic photons through tissue.
Mirrors, lenses, beam splitters, optical fibers and filters are used together to use measure ballistic photons. ex: Optical Coherence Tomography (OCT) Strength: High resolution Weakness: Can't penetrate through deep tissues
38
Define Structural Coloration
Colour visible on an object due to its structure, not luminescence. ex: Butterfly wings using gratings
39
Incident Angle of Acceptance in optical fibers
sinθ = √ (n1^2 - n2^2 )