LECTURE 1 (Intro)

Question 1

Characteristics of plasmids

Answer 1

• extrachromosomal piece of DNA (acts independently)
• normally circular
• smaller than chromosome
• versatile (used for cloning)
• antibiotic resistance

Question 2

What is a phagemid?

Answer 2

Phagemid: plasmid with a ori from a bacteriophage that allow ss replication and packaging into the phage. (it also has a ds ori needed for ds replication)

Question 3

What is a cosmid and a cosa site?

Answer 3

Plasmid with a cos site from a bacteriophage. The cos site is a sequence essential for packaging.

Question 4

What is a fosmid?

Answer 4

A cosmid based on a F’ plasmid. it has a sequence that allows it to integrate into the bacterial chromosome via homologous recombination.

Question 5

BAC?

Answer 5

Bacterial Artificial Chromosome. DNA construct + F plasmid. used for cloning.

Question 6

YAC?

Answer 6

Yeast Artificial Chromosome. construct that comes from yeast. 1stly used for cloning but BACs work better.

Question 7

3 types of plasmids.

Answer 7

pUC: plasmid University of California
pBR322: amp and tet resistance. archetypical vector.
pTOL: found in pseudomonas (bact) responsible of carbon metabolism

Question 8

Do plasmids autonomously replicate?

Answer 8

yes

Question 9

What are genomics?

Answer 9

The study of whole genomes