Lecture 10.27.14 (Exam #3)

Question 1

If there is oxygen present then pyruvate is sent to_____

Answer 1

citric acid cycle

Question 2

The outer membrane of the mitochondria is_____

Answer 2

very purous

Question 3

The inner membrane of the mitochondria is_____

Answer 3

tightly sealed and needs transporters to get molecule across

Question 4

Inner membrane space

Answer 4

space between the inner and outer membrane

Question 5

Matrix

Answer 5

space inside the inner membrane

Question 6

What enzyme moves PA from the inner membrane space to the matrix?

Answer 6

pyruvate translocase

Question 7

What also happens when PA is moved into the matrix?

Answer 7

H+ is moved into the inner membrane space

Question 8

Antiport

Answer 8

One molecule in while another molecule is sent out

ex.PA and OH-

Question 9

PA is converted to Acetyl CoA and CO2 by which enzyme?

Answer 9

Pyruvate decarboxylase complex

Question 10

CoA

Answer 10

a mobile carrier of carbons

Question 11

enzyme complex

Answer 11

group of enzymes, proteins, cofactors that are all coupled together

Question 12

How many enzymes are apart of PDH?

Answer 12

5

Question 13

What are the jobs of the 5 enzymes of PDH?

Answer 13

3 used for cataylsis

2 regulate later

Question 14

3 catalytic enzymes of PDH?

Answer 14

pyruvate dehydrogenase (E1)
acetyl transferase (E2)
dehydrogenase (E3)

Question 15

Pyruvate dehydrogenase does what and what is its PG?

Answer 15

redox

TPP

Question 16

Acetyl transferase does what and what is its PG?

Answer 16

transfer functional group

lipoamide

Question 17

Dehydrogenase (E3) does what and what is its PG?

Answer 17

redox

FAD

Question 18

Pyruvate Dehydrogenase binds ____ to ___ and CO2 is lost.

Answer 18

acetate group to TPP

decarboxylation and alpha cleavage

Question 19

Acetyl transferase (E2)’s ______ takes acetate’s electrons in E1 active site

Answer 19

lipoamide

Question 20

Lipoamide

Answer 20

Takes acetate’s E+ and transfer acetate to CoA in E2 active site

Question 21

Where are electrons(E2) transferred to FAD?to make?

Answer 21

in E3 active site; FADH2

Question 22

In dehydrogenase (E3) FADH2 transfers electrons to

Answer 22

NAD+

Question 23

Can FADH2 go into the ETC

Answer 23

no

Question 24

2 regulatory enzyme of PDH

Answer 24

pyruvate dehydrogenase kinase

pyruvate dehydrogenase phosphotase

Question 25

PDH kinase

Answer 25

puts Pi on E1 active site (SLOWS PDH)

Question 26

PDH phosphotase

Answer 26

takes Pi off E1 active site (PDH GO)

Question 27

What are 2 ways to regulate PDH

Answer 27

competitive inhibition | kinase/phosphotase

Question 28

When kinase is active (4)

Answer 28

``` SLOWS PDH high concentration of ATP lipoamide is reduced high concentration of NADH high concentration of Acetyl CoA ```

Question 29

When phosphotase is active (3)

Answer 29

PDH GO high concentration of ADP/AMP high concentration of Mg+2 high concentration of Ca+2