Lecture 11 Flashcards
(37 cards)
Antibodies in plasma or serum
group A
has anti-B
Antibodies in plasma or serum
group B
has anti-A
Antibodies in plasma or serum
group AB
has neither
Antibodies in plasma or serum
group O
has anti-A and anti-B
Looking for presence or absence of antigens on the surface of RBCs
group A
has A antigen 40% of population
antigens on the surface of RBCs
group B
has B antigen 11%
antigens on the surface of RBCs
group C
has A and B 4 %
antigens on the surface of RBCs
group O
has neither A nor B 45 % (most common)
Blood Grouping
sample:
EDTA (lavender, pink )or red top; do not separate, refrigerate
• direct or forward grouping looking for antigen on the surface
1 drop RBCs + 1 drop anti-A or anti-B
• reverse or indirect grouping when looking for the antibody :
patient serum with commercial A or B cells(test for blood group antibodies)
Other than the ABO and Rh blood group systems
some 20 blood group systems, such as Duffy, Kidd and Kell, on human red cells.
Antigens on surface of RBCs – D is acquired not naturally in the body
Rh D+
has D antigen 85 % of population
Antigens on surface of RBCs – D is acquired not naturally in the body
Rh D-
lack D antigen
Antigens on surface of RBCs – D is acquired not naturally in the body
Rh Du
weak Rx but is D+ rare
Antibodies: IgG type, occur after exposure to antigens
Rh D+
lacks anti-D
Antibodies: IgG type, occur after exposure to antigens
Rh D-
anti-D if exposed to D Ag by transfusion or pregnancy
Rh Typing
Patient RBCs + anti-D, heated on slide box to encourage agglutination by IgG
Gel (solid Phase) Testing for Blood Group
- Card is pre –filled with gel and mixed with appropriate reagent
- Diluents of patients’ cells pipette on to gel and card incubated
- Card centrifuged and red
- Agglutination stays on top of gel
- No agglutination goes to the bottom
Look at slide
agglutination ring on top and no agglutination at the bottom
A genotype:
the genes inherited from each parent’s blood group which are present on the chromosomes.
A phenotype:
the observable effect of the inherited genes: that is, the blood group itself.
The A and B genes
are dominant over the O gene and the phenotype A
can therefore arise from either the AO or the AA genotype.
Similarly, the phenotype B can arise from either the BO or the BB genotype.
look at slide for geno and phenotype
Haemolytic Disease of the Newborn (HDN):
when mother is Rh (–) and fetus is Rh (+); fetal cells enter maternal circulation during delivery
mother develops anti-D (IgG) which destroys fetal RBCs in subsequent pregnancies
mother given injection of RhD immunoglobulin (RhoGAM, RhIG) within 72 hrs of delivery to neutralize RhD antigens on fetal cells
Cross Match (Blood Compatability Testing) Specimen:
Pink, lavender or red top; do not separate strict adherence to patient identification protocols