Lecture 2 Flashcards
(21 cards)
What are the 3 domains of the phylogenic tree of life?
Bacteria
Archaea
Eucaryota
There are different shapes of bacteria
Name the major components of gram positive bacteria (5)
Peptidoglycan
Cell membrane
Teichoic acid
Lipoteichoic acid
Membrane proteins
Teochoic acid (TA) vs lipoteochoic acid (LTA)
Anionic polymers
Made of polyglycerol phosphate units
Involved in cell morphology and division
On surface
Adhesion amphiphile (water and fat loving)
Regulates autolytic wall enzymes
Name the major components of Gram negative bacterial cell membrane (9)
Membrane protein
Phospholipids
Porins
Lipopolysachharides
Outer membrane layer
Peptidoglycan
Periplasmic space
Cell membrane
Lipoproteins
What is the function of the inner cell membrane?
Physical barrier around cell
Control what comes in and out (selective permeability)
Name components of fluid mosaic model cell membrane
Hydrophilic region
Hydrophobic region
Transport protein
Peptidoglycan
Alternating NAG and NAM
Chains connected with oligopeptide
What is the function of the cell wall (Peptidoglycan)
Cell structure, shape, integrity, rigidity
Antibacterial?
Periplasm
Gel-like matrix
Acid fast organisms
Wax like
Nearly impermeable membrane
Contain my colic acid, complex lipids, fatty acids, waxes
Highly resistant to disinfectants and dry conditions
My colic acid
Long fatty acids
Form major component of cell wall
Mycoplasma
Smallest prokaryotes
No cell wall
Can’t retain crystal violet iodine
-therefore gram stain not part of I’d
More than 100 species
Endospores
In gram positive
A survival mechanism
Retractable, thick walled
Pathogenesis
When an infection leads to disease
Specialized structuras
Sediment sampling
Membrane filter apparatus
Filter support
Filter 0.45 um
Put filter in medium
Incubation for a day
Coliform colony
Culture base- counting
Get organism
Culture sample in agar
Count colony
Microscopic cell counting
Serial dillution
Photon counting tv camera
Rapid detection and counting of viable bacteria
