lecture 2

Question 1

what does not cause replication and transcription to stop?

Answer 1

misincorporation or deamination

Question 2

do the following stop replication or transcription? dimers, alkylation, ss nick, removal of base

Answer 2

yes

Question 3

how are thymine dimers fixed?

Answer 3

excision or direct (rare)

Question 4

does depurintation stop replication and transcription? corrected by what

Answer 4

yes, insertion

Question 5

what DNA pol is used in excision?

Answer 5

DNA pol 1

Question 6

what are major pathways of repair?

Answer 6

uvr, dam methyl-dircted, recBC recF

Question 7

what percent of uvr is short patch vs long patch?

Answer 7

99, 1

Question 8

long patch in uvr can remove how many nt around where

Answer 8

1500-9000. fork

Question 9

what uses XP excision repair genes?

Answer 9

NER

Question 10

NER uses what TF?

Answer 10

TF11H

Question 11

XPD is called what in yeast?

Answer 11

RAD4

Question 12

XPC called what in yeast?

Answer 12

XPC

Question 13

in BER what comes first?

Answer 13

glycosylase, which can be followed by lyase step

Question 14

what happens when lyase happens in BER?

Answer 14

APE recruits beta which then uses ligase and make short

Question 15

what happens when lyase doesnt happen?

Answer 15

APE does the nicking, and then delta epsilon recruits with use of FEN endo. makes long

Question 16

base flipping used by what

Answer 16

methylases and glycoslyase

Question 17

what has pyrimidine dimer recognition?

Answer 17

rad4

Question 18

examples of mutator genes

Answer 18

mut SLH and dna Q

Question 19

dna Q is what

Answer 19

polymerase 3 subunit (epsilon)

Question 20

dam methylation used in what process?

Answer 20

mismatch

Question 21

which exo used in mismtach

Answer 21

5’-3 exo 7 or 3’-5’ exo

Question 22

Mut S/L removes what?

Answer 22

T from GT and CT

Question 23

MutY removes what and encodes what

Answer 23

adenosine glycosylase. A from CA and GA

Question 24

what hydrolyzes 8-oxo-dGTP

Answer 24

mutT

Question 25

what removes G=O that is replaiced with C

Answer 25

mutM

Question 26

what removes A that is repaired with G=O

Answer 26

mutY

Question 27

8-ox G pairs with what

Answer 27

A

Question 28

MSH and MutS/L are homologous and fix hat

Answer 28

mismatch base pairs

Question 29

what complex binds insertion/deletion loops resulting from replication slippage

Answer 29

msh2/3

Question 30

what complex binds single base mismatch?

Answer 30

msh2/6

Question 31

what repairs replication slippage?

Answer 31

MutS and mutL

Question 32

what is used to retart replication forks? what is used in repairing gaps due to TT dimers in diaghter strand for replication?

Answer 32

recBC and recA. RecF, recO, and recR

Question 33

what in NHEJ does the trimming?

Answer 33

attremis

Question 34

uvrB is it constitutive ?

Answer 34

no its inducable

Question 35

where does rec A cleave?

Answer 35

ala-gly

Question 36

is SOS response reversible?

Answer 36

yes