Lecture 2 Questions Flashcards Preview

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Flashcards in Lecture 2 Questions Deck (5)
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1
Q

At physiologic pH (7.4), what is the charge on the side chain of Glu? Of Arg?

A

Negative; positive

2
Q

In a soluble protein, would Leu would most likely be located on the inside or the outside of the folded protein? What if it were a membrane protein?

A

Inside; in the portion of the protein spanning the membrane

3
Q

Make sure you can identify an alpha helix and a beta sheet in a diagram.

A

diagrams…

4
Q

In the collagen alpha chain (see lecture powerpoint), what amino acid repeats every third position? Why is it special? (from Flashcards)

A

Gly; fits into the small space because it only has H for a sidechain

5
Q

What can a Western blot tell you about a protein?

A

If it is present in the sample, approximate size (M.W.), whether there are multiple modified forms (sometimes). It does not say anything about protein structure or activity.