Lecture 3: Genes and Transcription Flashcards Preview

MCM > Lecture 3: Genes and Transcription > Flashcards

Flashcards in Lecture 3: Genes and Transcription Deck (10):
1

RNA Polymerase 1

Transcribes rRNA genes

2

RNA Polymerase 2

Transcribes mRNA/protein coding genes

3

RNA Polymerase 3

Transcribes tRNA

4

5 Transcription Factors for RNA Polymerase 2

Order: TFIID, TFIIB, (RNA Poly 2), TFIIF, TFIIE, TFIIH
DAB Paul Twice For Efficient Healing

5

TFIID

Recognizes TATA box with TBD subunit and and starts the process of transcription.

6

TFIIB

Positions the RNA Polymerase. Second ste.

7

TFIIF

Stabilizes RNA Pol and attracts TFIIE and TFIIH

8

TFIIE

Recruits TFIIH

9

TFIIH

Unwinds DNA at transcript start point. Phosphorylates Ser5 of RNA pol CTD (carboxyterminus domain), releases RNA pol from promoter

10

Enzymes used for creating 5' cap

Phosphatase=removes phosphate from 5’ end
Guanotransferase= adds gmp in reverse linkage
Methyl transferase= adds methyl group to guanosine