Lecture #4: Chart terms Flashcards by Michelle Ann

Neutrophils

[Circulating effector cells] Early phagocytosis and killing of microbes.

How well did you know this?

Not at all

Perfectly

Macrophages

[Circulating effector cells] Efficient phagocytosis and killing of microbes, secretion of cytokines that stimulate inflammation.

How well did you know this?

Not at all

Perfectly

NK Cells

[Circulating effector cells] Lysis of infected cells, activation of macrophages.

How well did you know this?

Not at all

Perfectly

Complement

[Circulating effector proteins] Killing of microbes, opsonization of microbes, activation of leukocytes

How well did you know this?

Not at all

Perfectly

Mannose-binding lectin (collectin)

[Circulating effector proteins] Opsonization of microbes, activation of complement (lectin pathway)

How well did you know this?

Not at all

Perfectly

C-reactive protein (pentraxin)

[Circulating effector proteins] Opsonization of microbes, activation of complement

How well did you know this?

Not at all

Perfectly

TNF, IL-1, chemokines

[Cytokines] Inflammation

How well did you know this?

Not at all

Perfectly

IFN-alpa, -beta

[Cytokines] Resistance to viral infection

How well did you know this?

Not at all

Perfectly

IFN- gamma

[Cytokines] Macrophage activation

How well did you know this?

Not at all

Perfectly

IL-12

[Cytokines] IFN-gamma production by NK cells and T cells

How well did you know this?

Not at all

Perfectly

IL-15

[Cytokines] Proliferation of NK cells

How well did you know this?

Not at all

Perfectly

IL-10, TGF-beta

[Cytokines] Control of inflammation

How well did you know this?

Not at all

Perfectly

TLR-1, -2, -4, -5, -6

Recognize extracellular pathogens (bc some present on cell surface)

How well did you know this?

Not at all

Perfectly

Endosomal TRL’s respond only to ____.

Nucleic acids

How well did you know this?

Not at all

Perfectly

TLR-3, -7, -8, -9

Recognize intracellular pathogens

How well did you know this?

Not at all

Perfectly

[Receptor] TLR1: TLR2 heterodimer – what ligands?

Lipopeptides, GPI

How well did you know this?

Not at all

Perfectly

[Receptor] TLR1: TLR2 heterodimer – what microorganisms recognized?

Bacteria, Parasites

How well did you know this?

Not at all

Perfectly

[Receptor] TLR1: TLR2 heterodimer – what cells carrying receptor?

Monocytes, DC’s, eosinophils, basophils, mast cells

How well did you know this?

Not at all

Perfectly

[Receptor] TLR1: TLR2 heterodimer – what cellular location?

Plasma membrane

How well did you know this?

Not at all

Perfectly

[Receptor] TLR2: TLR6 heterodimer – what ligands?

Lipoteichoic acid, zymosan

How well did you know this?

Not at all

Perfectly

[Receptor] TLR2: TLR6 heterodimer – what microorganisms recognized?

Gram-positive bacteria, yeasts (fungi)

How well did you know this?

Not at all

Perfectly

[Receptor] TLR2: TLR6 heterodimer – what cells carrying receptor?

Monocytes, DC’s, eosinophils, basophils, mast cells

How well did you know this?

Not at all

Perfectly

[Receptor] TLR2: TLR6 heterodimer – what cellular location?

Plasma membrane

How well did you know this?

Not at all

Perfectly

[Receptor] TLR3 – what ligands?

Double-stranded viral RNA

How well did you know this?

Not at all

Perfectly

[Receptor] TLR3 -- what microorganisms recognized?

Viruses

[Receptor] TLR3 -- what cells carrying receptor?

NK cells

[Receptor] TLR3 -- what cellular location of receptor?

Endosomes

[Receptor] TLR4:TLR4 homodimer -- what ligands?

Lipopolysaccharide

[Receptor] TLR4:TLR4 homodimer -- what microorganisms recognized?

Gram-negative bacteria

[Receptor] TLR4:TLR4 homodimer -- what cells carrying receptor?

Macrophages*, DC's, Mast cells, Eosinophils

[Receptor] TLR4:TLR4 homodimer -- what cellular location of receptor?

Plasma membrane

[Receptor] TLR5 -- what ligands?

Flagellin

[Receptor] TLR -- what microorganisms recognized?

Motile bacteria having a flagella

[Receptor] TLR5 -- what cells carrying receptor?

Intestinal epithelium

[Receptor] TLR5 -- cellular location of receptor?

Plasma membrane

[Receptor] TLR7 -- what ligands?

Single-stranded viral RNA's

[Receptor] TLR7 -- what microorganisms recognized?

Viruses (HIV)

[Receptor] TLR7 -- what cells carrying receptor?

Plasmacytoid DC's, NK cells*, eosinophils, B cells

[Receptor] TLR7 -- what cellular location of receptor?

Endosomes

[Receptor] TLR8 -- what ligands?

Single-stranded viral RNA's

[Receptor] TLR8 -- what microorganisms recognized?

Viruses

[Receptor] TLR8 -- what cells carrying receptor?

NK cells

[Receptor] TLR8 -- what cellular location of receptor?

Endosomes

[Receptor] TLR9 -- what ligands?

Unmethylated CpG-rich DNA

[Receptor] TLR9 -- what microorganism recognized?

Bacteria, Viruses (herpes)

[Receptor] TLR9 -- what cells carrying receptor?

Plasmacytoid DC's, basophils*, eosinophils, B cells

[Receptor] TLR9 -- what cellular location of receptor?

Endosomes

[Receptor] TLR10 homodimer and heterodimers with TLR1 and 2 -- what ligands/microorganisms recognized?

Unknown

[Receptor] TLR10 homodimer and heterodimers with TLR1 and 2 -- cells carrying receptor?

Plasmacytoid DC's, basophils, eosinophils, B cells | Same as TLR9

[Receptor] TLR10 homodimer and heterodimers with TLR1 and 2 -- what cellular location of receptor?

Unknown

Bacteria

TLR's 1, 2, 4, 5, 9

Viruses

TLR's 3, 7, 8, 9

Fungi

TLR's 2, 6

1. TRIF dependent signaling

TLR3 uses only the adapter protein TRIF to activate NF-kB and IRF's transcription proteins

2. MyD88/TRIF dependent signaling

TLR4 use both MyD88 and TRIF to activate NF-kB and IRF's

3. MyD88 dependent signaling

TLR's 1, 2, 5, 6, 7, 8, and 9 use only the adapter protein MyD88 to activate the transcription factors NF- kB and interferon-regulatory factor (IRF) transcription proteins

TRIF?

TIR (Toll/IL-1 Receptor)- domain- containing adapter- inducing interferon-B

NF- kB

Nuclear factor kB

IRF

Interferon regulatory factors

[Keys] LPS

Lipopolysaccharide

[Keys] LBP

LPS binding protein in the blood

[Keys] CD14

Receptor for LPS; myeloid cell differentiation marker

[Keys] MD2

LPS binding protein that make a "bridge' : CD14-LPS-MD2-TLR4

[Keys] MyD88

Myeloid differentiation primary response gene 88

[Keys] TIR Domain

Toll/Interleukin-1 receptor domain

IRAK4

Interleukin-1 Receptor- Associated Kinase 4

TRAF6

TNF Receptor Associated Factor 6

IKK

I kappa B Kinase

TLR's- 1,2,4,5,6

Extracellular and membrane receptors

TLR's 3,7,8,9

Intracellular, endosomal receptors, respond to nucleic acids

The scavenger receptor (SR) family consists of?

1. Scavenger receptor class A type I 2. Scavenger receptor class A type II 3. MARCO- Macrophage Receptor with Collagenous Structure

Oxidized lipoproteins can lead to

Atherosclerosis

Inflammation is an innate reaction caused by:

1. An increased blood supply to the affected area (redness and heat) 2. An increased capillary permeability >> leak from the blood vessels (swelling and pain) 3. A massive influx of neutrophils in the tissue 4. An arrival of monocytes/macrophages (16-48 hrs) 5. Distortion of the homeostasis and loss of fxn

HMGB l (high mobility group box 1)

Is a protein passively released during necrosis

HMGB I is recognized by

Recognized by RAGE: • Activates NF-kB pathway • RAGE is receptor for HMGBl

URIC ACID

Another diffusible danger signal

URIC ACID is recognized by:

Recognized by NLRP3: | • Activates NIF-KB pathway

HSP's

Another danger signals

HSP's are recognized by

Recognized by scavenger receptor class A • HSPs induce NF-kB pathway and release of inflammatory cytokines (TNF-a and IL-1).

MANNOSE RECEPTORS

C-type lectin receptor that recognizes terminal D-mannose, L-fucose, and N-acetyl-Dglucosamine sugars

Lecture #4: Chart terms Flashcards

(80 cards)