Lecture 4.3 Flashcards
What are structures that are included in the post synaptic density?
Ligand gated channels
Anchoring proteins- to anchor receptors
Cytoskeleton-structure
Regulatory proteins
Does glutamate have a thin or thick post synaptic density?
Thick
GABA has a thin or thick post synaptic density?
Thin
What are cell adhesion molecules?
They hold pre and post synaptic structures together
What is the role of Neuroligins?
They organise the post synaptic density
Give an example of a cell adhesion molecule and what do they bind to
Neuroligins and they bind to Neurexins
What is the difference between Neuroligin 1, Neuroligin 2, Neuroligin 3 and Neuroligin 4?
Neuroligin 1- in ALL glutamatergic synapses
Neuroligin 2- in SOME inhibitory synapses
Neuroligin 3- in some excitatory AND inhibitory synapses
Neuroligin 4-in glycinergic synapses in retina (Amacrine cells)
What do Neuroligins bind to postsynaptically?
PSD95- Postsynaptic density protein 95
How many binding domains does PSD95 have and what are they called as a group?
3 PDZ binding domains
Which binding site do Neuroligins bind to on PSD95?
They bind via the 3rd PDZ domain at one end
Describe the binding receptors at each of the 3 PDZ domains
1st- AMPA glutamate receptor
2nd-AMPA glutamate receptor
3rd-Neuroligins bind
What doed PSD95 bind to?
NMDA receptors
Calcium calmodulin protein kinase II
Neuronal nitric oxide synthase
Shank proteins via GKAP
What does CaMKII (Calcium-calmodulin protein kinase II) do?
Regulates NMDA and AMPA cycling
Memory function
What 2 things do Shank proteins bind to?
PSD95 indirectly via GKAP
Neuroligins- mutations in this can lead to Autism
Where are Shanks not found?
In GABA synapses
