Flashcards in Lecture 5 - RH Deck (18):
Why has the human genome been sequenced?
Molecular medicine: Diagnosis, prevention and treatment
Reproductive biology: How do we develop?
Hereditary disease: Here are 3,000-4,000 hereditary disease Cure???
Understanding of genetic alterations (e.g. SNPs)
Idea to first sequence the entire human genome was proposed at a scientific meeting sponsored by U.S. Dept of Energy
US congress approves funding to Dept of Energy and NIH, James Watson named director of NIH NHGR
Project officially begins (International consortium US, France, England, China and Germany)
1990 - 1995
Human Genome project focuses on building maps
Francis Collins replaces James Watson as Director
Bermuda principles established for IHGSC
J. Craig Venter forms Celera (aim to sequence entire genome in 3 years)
Public consortium on track to have draft of human sequence by 2000
Chromosome 22 completed and published
Chromosome 21 completed and published
Both groups announce completion of Draft
IHGSC publish in Nature
Celera publish in Science
What is the difference between the 2 consortia; IHGSC and Celera?
IHGSC public funded
Used hierarchical approach
Easier because different groups were sequencing different chromosomes
Whole genome shotgun
Why was chromosome 22 sequenced first?
How does hierarchal sequencing work?
Bacterial Artificial Chromosomes (BACs) are generated