Lecture 5 - RH Flashcards Preview

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Flashcards in Lecture 5 - RH Deck (18):
1

Why has the human genome been sequenced?

Molecular medicine: Diagnosis, prevention and treatment
Reproductive biology: How do we develop?
Hereditary disease: Here are 3,000-4,000 hereditary disease Cure???
Understanding of genetic alterations (e.g. SNPs)

2

1984-1986

Idea to first sequence the entire human genome was proposed at a scientific meeting sponsored by U.S. Dept of Energy

3

1988

US congress approves funding to Dept of Energy and NIH, James Watson named director of NIH NHGR

4

1990

Project officially begins (International consortium US, France, England, China and Germany)

5

1990 - 1995

Human Genome project focuses on building maps

6

1992

Francis Collins replaces James Watson as Director

7

1996

Bermuda principles established for IHGSC

8

1998

J. Craig Venter forms Celera (aim to sequence entire genome in 3 years)

9

1999

Public consortium on track to have draft of human sequence by 2000

10

Dec ’99

Chromosome 22 completed and published

11

May ’00

Chromosome 21 completed and published

12

Jun ’00

Both groups announce completion of Draft

13

15/2/01

IHGSC publish in Nature

14

16/2/01

Celera publish in Science

15

What is the difference between the 2 consortia; IHGSC and Celera?

IHGSC public funded
Used hierarchical approach
Easier because different groups were sequencing different chromosomes

Celera
Whole genome shotgun
Finished faster

16

Why was chromosome 22 sequenced first?

Shortest chromosome

17

How does hierarchal sequencing work?

Bacterial Artificial Chromosomes (BACs) are generated

18

How does shotgun sequencing work?

Whole-genome shotgun reads are produced