Lecture 6 Flashcards
(17 cards)
What happens during transcription
It the 1st part when a gene is transcribed into mRNA which is then translated into a protien
What are proteins
Polypeptide chains of amino acids
What are genes
They are short sequences of DNA that contain genetic instructions to produce an RNA molecule and/or protein
What is genetic code
It refers to a triplet sequence of ribonucleotides that codes for a specific amino acid
Examples of proteins
Fibrous proteins
Digestive enzymes
Metabolic enzymes
Immune system
What is transcription
Portion of DNA nucleotide sequence copied into RNA nucleotide sequence. Occurs in the nucleus for eukaryotic cells and the cytoplasm of prokaryotic cells
What is translation
Synthesis of polypeptide under direction of RNA. Occurs in the cytoplasm of all cells
Transcription and translation in eukaryotes
1) DNA protected in nucleus therefore transcription occurs in the nucleus
2) But mRNA most exit the nucleus to go to the cytoplasm for translation
3) DNA contains non-coding DNA (introns) which must be cut out of DNA between the 2 processes therefore mRNA processing required
Transcription and translation in prokaryotes
1) No nucleus and DNA lies in the cytoplasm and therefore both processes occur in tandem (quickly). No transport needed
2) DNA is mostly coding (exons) and therefore processing of non-coding DNA is not required
Can genes be expressed with different efficiencies
Yes
Difference between DNA and RNA
RNA: single stranded short polymer that has a uracil base
DNA: double stranded long polymer that has a thyamine base
What is mRNA
RNA molecules copied from genes which direct synthesis of proteins
What are the RNA subtypes
Transfer RNA (tRNA)
ribosomal RNA (rRNA)
small nuclear RNA (snRNA)
What’s the most abundant RNA subtype
rRNA, it fractions into 2 band, 28s and 18s
What dose transcription produce
RNA complementary to one strand of DNA
Whar does transcription being with
The opening and unwinding of small small portion of double helix to expose bases on each DNA strand