Lecture 7 Connective Tissue I

Question 1

What is connective tissue?

Answer 1

Supports and connects other tissue (parenchyma) and forms a matrix that maintains form/shape of the organ

Question 2

WHat are the functions of connective tissue?

Answer 2

transport (vascularized – blood supply) Mechanical support Growth & repair Hemochoasis (blood cell synthesis) Energy economics (fat, adipose tissue)

Question 3

______ and ______ make up the ECM

Answer 3

protein fibers and ground substance

Question 4

What are the 4 cellular components of CT?

Answer 4

fibroblast, adipocyte, macrophage, mesenchymal cell

Question 5

What is the origin of CT?

Answer 5

Embryonic mesenchyme

Question 6

______ have large nuclei in cells, euchromatic, tend to have a prominent nucleolus and surrounded by an ECM that they produced and that consists largely of a simple ground substance rich in hyaluronan with very little collagen.

Answer 6

Embryonic mesenchyme

Question 7

What are the 6 types of CT tell types?

Answer 7

fibroblasts, macrophages, mast cells, plasma cells, adipocytes, leukocytes

Question 8

_____ are active cells with lots of collagen and spindle shaped nucleus.

Answer 8

Fibroblasts

Question 9

What does this image depict?

Answer 9

Active and inactive fibrocytes

Question 10

Fibroblasts involved in wound healing call ______. Contractile. Pull edges of the wound together by contraction like smooth muscle.After role completed, undergo ______

Answer 10

myofibroblasts, apoptosis

Question 11

_______ are derived from monocytes and have a prominent nucleus as well as a lot of lysosomes. Also, have antigen presentation for immune response.

Answer 11

Macrophages

Question 12

______ are derived from progeniture cell in bone marrow, take up residency in CT and play a role in inflammation. Use a metachromatic stain.

Answer 12

Mast cells

Question 13

True or False: Mast cell secretion is triggered by re-exposure to certain antigens and allergens (ie, bacteria)

Answer 13

True

Question 14

______ are antibody-secreting plasma cells, derived from b-lymphocytes, very active (RER–Golgi), basophilic

Answer 14

Plasma cell (can see Golgi ghosts in cell)

Question 15

What are the 3 fiber types?

Answer 15

Collagen, reticular, elastic

Question 16

What percent of protein in your body is collagen?

Answer 16

30%

Question 17

_____ 28 different types, resistant to tears and shearing forces, a key element to the basement membrane

Answer 17

Collagen

Question 18

______ are elongated components of the connective tissue of CT, that are formed as precursors that polymerize externally and not formed in cell

Answer 18

Fibers

Question 19

______ are delicate connective tissue, form networks

Answer 19

Reticular

Question 20

_____ aid in providing resiliency to connective tissue.

Answer 20

Elastic fibers

Question 21

90 % of collagen is ______, acidophilic/eosinophillic, extremely strong and forms bundles

Answer 21

Type I

Question 22

What type of assembly does Type I collagen form?

Answer 22

Triple helix

Question 23

_____ form stroma and lattices/networks, high in carbohydrate (PAS) but see well with silver stain

Answer 23

Reticular fibers

Question 24

______ is a thinner Type I collagen, that stains poorly with H&E and better with aldehyde fuchsin (ex, dermin and aorta)

Answer 24

Elastic fibers

Question 25

True or False: When stretched, elastic binds with less steric hindrance

Answer 25

True

Question 26

\_\_\_\_\_\_ is highly hydrated and transparent, a complex mixture of macromolecules.

Answer 26

ground substance

Question 27

What are the 3 types of ground substance?

Answer 27

Glycosaminoglycans (GAG) Proteoglycans Multi adhesive glycoproteins

Question 28

What are examples of glycoproteins and proteoglycans?

Answer 28

Glyco- More protein than carb, Oligosaccharides, Multi adhesion proteins Proteo - carb than protein, Polysaccharides, GAG glycosaminoglycan = mucopolysaccharide, Hyaluronic acid most common, binds a lot of water ​

Question 29

\_\_\_\_\_ is a multi adhesive protein present in the basal lamina, a binding site for integrins (epithelial cell) + matrix macromolecules

Answer 29

Laminin

Question 30

\_\_\_\_\_ is a multi-adhesive protein, binds to GAG, found throughout CT

Answer 30

Fibronectin

Question 31

what are the 4 types of proper connective tissue?

Answer 31

Loose, Dense (regular, irregular), reticular, mucoid

Question 32

This picture illustrates what type of connective tissue? (dense w/ less GS)

Answer 32

Dense regular

Question 33

\_\_\_\_\_\_: high fiber - Irregular orientation, eg, dermis of skin (picture b) - Collagen blue trichrome stain - Sheer forces from different directions = irregular orientation \_\_\_\_\_\_: less fiber, less dense between DI + epithelium - Well vascularized (b/c epithelium not, needs nutrients and blood supply somehow) - Delicate - More GS ​

Answer 33

Dense irregular, Loose CT

Question 34

This is an example of what kind of connective tissue? (silver stain)

Answer 34

reticular

Question 35

\_\_\_\_\_\_ tissue is, similar to embryonic mesenchyme, Very loose ECM, Little fibers, No eosin staining of collagen, Mainly GS, Very watery tissue, HYALURONAN = gelatinous

Answer 35

Mucoid