Lecture 8 & 9 - Human Genome 2

Question 1

______________ involves sequential addition of nucleotides until one nucleotide without a 3’-0H is incorporated into the DNA strand, stopping synthesis. By knowing which nucleotide is missing the 3’-0H group, and by being able to detect that nucleotide independently of the others, it is possible to piece together a sequence of the template used for the synthesis.

Answer 1

Dideoxy or Sanger sequencing

Question 2

Next-Gen sequencing including amplification of DNA fragments covalently attached to a chip. Each of the fragments is eventually sequenced by photographing the chip as each, color-labeled nucleotide is incorporated

Answer 2

Massively Parallel

Question 3

Modern sequencing methods can generate about _________ of sequence per day.

Answer 3

100s of millions

Question 4

When a _________ sequence is available, large scale “re-sequencing” can be used to identify variations against the reference sequence.

Answer 4

Reference

Question 5

When a genome is being sequenced for the first time, _________ are used to generate contigs, to add an extra level of certainty to the organization of the final sequence.

Answer 5

BAC Clones