Lecture 9: GABA

Question 1

Enzyme that makes Gaba from Glutamate

Answer 1

Glutamic Acid Decarboxylase

Question 2

GAD inhibitor (less inhibition leads to seizures)

Answer 2

Allyglycine

Question 3

GAD Located on terminals and membranes

Answer 3

GAD 65

Question 4

GAD Located everywhere

Answer 4

GAD 67

Question 5

Co-factor in the synthesis of GABA (HELPS OU GAD)

Peeps with deficiencies are at high risk for seizures

Answer 5

Vitamin B6 (PYROXIDINE)

Question 6

Takes up GABA into vesicles

Answer 6

VGAT

Question 7

Removes GABA from cleft (transporter). On neurons and astrocytes.

Answer 7

GAT-1/ GAT-2

Question 8

Removes GABA from cleft (transporter). Only on astrocytes.

Answer 8

GAT-3

Question 9

GAT-1 inhibitor. Enhances inhibition; helps with seizures

Answer 9

Tiagabine (Gabitril)

Question 10

Enzyme that breaks down GABA

Answer 10

GABA-T (GABA amino-transferase)

Question 11

Metabolite of GABA

Answer 11

Succinate

Question 12

By product of GABA metabolism

Answer 12

Glutamate

Question 13

Irreversible inhibitor of GABA-T. Anticonvulsant but can lead to visual field problems

Answer 13

Vigabatrin (Sabril)

Question 14

GABAergic locations

Answer 14

Widespread; cerebral cortex, hippocampus, sub. nigra, striatum, globus pallidus, olfactory bulbs, cerrebellum

Question 15

GABA receptor

Answer 15

GABA-A

Question 16

of subunits on GABA A receptor

Answer 16

5

Question 17

Types of subunits on GABA A receptor

Answer 17

alpha, beta, gamma, delta

Question 18

GABA A receptors must contain at least..

Answer 18

2 alpha subunits, 2 beta subunits and 1 gamma OR 2 alpha, 1 beta and 2 gamma (sometimes replaced by delta)

Question 19

Best characterized binding site on GABA-A receptor

Answer 19

Benzodiazepine site (BzR)
NON COMPETATIVE --> cause not actually binding to GABA site but an alpha subunit

Question 20

alpha subunit responsible for sedation

Answer 20

alpha 1

Question 21

alpha subunit responsible for anxyiolitic effects

Answer 21

alpha 2/3

Question 22

alpha subunit responsible for cognition

Answer 22

alpha 3

Question 23

Non-competative antagonist of GABAA receptor

Answer 23

Picrotoxin

Question 24

GABAA agonist (leads to inhibition)

Answer 24

Muscimol

Question 25

GABAA competitive antagonist

Answer 25

Bicuculine

Question 26

CNS Depressant

Answer 26

Barbiturate

Question 27

Popular anxiolytic drugs implicated in DATE RAPE

Answer 27

Benzodiazepines (types are valium and xanax)