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BACTE (LESSON 18) > Legionella > Flashcards

Legionella Flashcards

1
Q

Ubiquitous gram-negative bacilli
and acquired through inhalation.

A

Legionella

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2
Q

Laboratory diagnosis for Legionella except:
A. Isolation using special media
B. Urine antigen detection
C. Direct fluorescent antibody
D. PCR
E. Serology

A

D

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3
Q

The most common pathogen of Legionella.

A

Legionella pneumophila

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4
Q

They have the ability to enter, survive, and multiply within host’s
cells,

A

Legionella / L. pneumophila

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5
Q

Most Legionella invades what macrophage?

A

bronchoalveolar macrophages

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6
Q

Legionella has the ability to produce ___.

A

proteolytic enzymes

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7
Q

T or F
Legionella spp. cause intracellular infections in humans but also survive in an extracellular environment.

A

True

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8
Q

Legionella can cause all the infections except:
A. BPF
B. Legionnaire’s Disease
C. Pontiac Fever
D. Asymptomatic Infection

A

A

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9
Q

Febrile disease with pneumonia brought by Legionella.

A

Legionnaire’s Disease

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10
Q

Febrile disease without pulmonary caused by Legionella

A

Pontiac Fever

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11
Q

Three major patterns of Legionnaire’s Disease.

A
  1. Sporadic
  2. Epidemic
  3. Nosocomial
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12
Q

Most common patterns of Legionnaire’s Disease and spread in comunity.

A

Sporadic

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13
Q

Short duration pattern of Legionnaire’s disease and has low attack rates

A

Epidemic

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14
Q

patterns of Legionnaire’s Disease in immunocompromised

A

Nosocomial

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15
Q

Caused by L. pneumophila that can be pneumonia (atypical).

A

Legionnaire’s Disease

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16
Q

A nonpneumonic form, short incubation period (2 days), Flulike symptoms caused by L. pneumophila

A

Pontiac fever

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17
Q

It occurs naturally in aquatic sources (lakes, rivers,
hot springs, mud)

A

Legionella

18
Q

T or F
Legionella can tolerate chlorine (resists water treatment)

A

True

19
Q

Major reservoir of Legionella.

A
  • Hot water systems
  • cooling towers
  • evaporative condensers
20
Q

Laboratory Diagnosis for Legionnaire’s disease.

A

Urine antigen detection + culture

21
Q

Lab. diagnosis for pontiac fever.

A

Serology

22
Q

Specimens for Legionella

A

– Sputum
– Bronchoalveolar lavage
– Bronchial washings

23
Q

Why saline or buffer should not be used in isolation of Legionella? (inhibitory effect
of sodium)

A

It has an inhibitory effect of sodium

24
Q

How many hours req. To refrigerate the specimens of Legionella for transportation

A

2 hours

25
Q

Characteristics of Legionella under microscope.

A

Pleomorphic, weakly staining, gram-negative bacilli.

26
Q

Since the Legionella is weakly stained in safranin, how many mins. Need to stain the organisms properly?

A

10 mins. For better absorption.

27
Q

weakly acid-fast; stains best with
modified Kinyoun procedure.

A

L. micdadei

28
Q

Confirmation test for Legionella.

A

DFA TEST (Direct Fluorescent Antibody Test)

29
Q

Common dye for DFA Test.

A

FITC (Fluorescein isothiocyanate) yellow green color

30
Q

Most important test for Legionnaire’s disease is __.

A

Culture

31
Q

_____ of specimens contaminated with other bacteria, such as sputum, before inoculation enhances isolation of Legionella spp.

A

Acid treatment

32
Q

Medium for Legionella spp. Requires ____ for growth.

A

L-cysteine

33
Q

Best medium for Legionella spp.

A

BCYE agar with L-cysteine

34
Q

True or false
Legionella is aerobic, fastidous, and does not grow in SBA.

A

True

35
Q

Appearance of Legionella spp. in BCYE.

A
  • Grayish-white or blue-green,
  • convex
  • glistening
36
Q

The central portion of young Legionella colonies has a _____ appearance, and the color is ___.

A

groundglass appearance”, light gray and granular

37
Q

Color of periphery of Legionella colonies.

A
  • pink
  • light blue
  • bottle green
    (bands with a furrowed appearance)
38
Q

All are common Phenotypic Characteristics of Legionella Species except:
A. Slow growth (3 to 5 days)
B. Catalase or oxidase: weakly (-)
C. ground-glass” colony morphology
D. Asaccharolytic

A

B.Catalase or oxidase: weakly (+)

39
Q

Urine Antigen test for Legionella.

A
  • RIA
  • EIA,
  • Immunochromographic assay
40
Q

___ test: LRT specimen; FITC-labeled (bright yellow to green)

A

DFA test

41
Q

3 rapid methods for identifying Legionella.

A
  • Urine Antigen test:
  • DFA test
  • DNA detection

BACTE (LESSON 18) (4 decks)

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