LESSON 2 LABORATORY SAFETY

Question 1

Why study safety?
- Affect the [?] and threaten the emotional health of the party involved
- Injuries are [?] in terms of lost wages & medical treatment
- Injuries impair the [?]

Answer 1

morale

expensive

ability to serve

Question 2

IS THE RESPONSIBILITY OF ALL

Answer 2

Laboratory Safety

Question 3

source of danger

Answer 3

Hazards

Question 4

“a condition or situation that exists in the work environment that could result in physical harm, injury and/ or damage”.

Answer 4

Hazards

Question 5

relates to exposure to the danger

Answer 5

Risks

Question 6

“the likelihood of a hazard causing harm”

Answer 6

Risks

Question 7

“the degree of likelihood that harm will be caused”

Answer 7

Risks

Question 8

Types of Hazards

Answer 8

B
P
E
G
C
R
P

Question 9

What are the hazards during the pandemic?
 Covid-19 positive [?]
 Patients who are [?]
 Covid-19 positive [?]
 Patients who [?] from Covid-19
 [?] in the hosp and lab
 Inadequate [?] for social distancing
 Lack of [?]
 Inadequate [?] of MTs
 Health professionals experiencing [?]
 Inadequate [?] and lack of coordination
 Health professionals getting [?]
 Lack of adequate[?] and (?)

Answer 9

patients

asymptomatic

body fluids

died

Overcrowding

space

facilities

training

fatigue

instruction

infected

ventilation; air control

Question 10

We all manage risk consciously or unconsciously - but rarely systematically

Answer 10

Risk Management

Question 11

Managing risk means [?]
Managing risk means [?]
Managing risk means [?]
Managing risk is all about [?]

Answer 11

forward thinking

responsible thinking

balanced thinking

maximising opportunity and minimising threats

Question 12

The [?] provides a framework to facilitate more effective decision making

Answer 12

Risk Management process

Question 13

Systematic application of management policies procedures and practices to the tasks of

Answer 13

Risk Management

Question 14

• Identifying and understanding the risk points

Answer 14

1. Analyzing risks

Question 15

• Assess based on probability and severity/impact

Answer 15

2. Evaluating risks

Question 16

• Pick the highest risk and decide how to mitigate them

Answer 16

3. Controlling risks

Question 17

• Check if the mitigation is effective

Answer 17

4. Monitoring risks

Question 18

Systematic approach to identify hazards, evaluate risk and incorporate appropriate measures to manage and mitigate ris for any work process or activity.

Answer 18

Risk Assessment

Question 19

How often must the risk assessment be reviewed?

• At least once every [?]
• After an [?]
• When there is [?] in work processes, (?) of new machinery or chemicals
• Information on (?) or requirement made known

Answer 19

3 years

accident

significant change; introduction

safety technology

Question 20

minimum infection prevention practices that apply to all patient care, regardless of suspected or confirmed infection status of the patient, in any setting where healthcare is delivered.

Answer 20

Standard Precautions

Question 21

Standard Precautions:

Answer 21

1. Hand hygiene
2. PPE 3
   . Safe handling of sharp instruments
3. Respiratory hygiene and cough etiquette
4. Good housekeeping
5. Proper waste disposal

Question 22

• Application of safety and containment measures to minimize or prevent exposure from infectious agents to the “person” handling it in the lab, building occupants and the environment

Answer 22

Biosafety

Question 23

• Prevents ACCIDENTAL release

Answer 23

Biosafety

Question 24

Protect “worker” from the bad bugs

Answer 24

Biosafety

Question 25

Foundation for biosecurity

Answer 25

Biosafety

Question 26

• Institutional security measures designed to prevent the loss, misuse, diversion, intentional/deliberate release of “pathogens and toxins”

Answer 26

Biosecurity

Question 27

• Prevents DELIBERATE release

Answer 27

Biosecurity

Question 28

Protect the “bugs” from bad workers

Answer 28

Biosecurity

Question 29

Combination of laboratory practices and procedures, safety equipment (primary barriers) and facilities (secondary barriers)

Answer 29

Biosafety Level

Question 30

: Bioseals and Double Walled and Ended Autoclaves

Answer 30

BSL 3 and 4

Question 31

is comprised of a steel or stainless steel plate that is continuously welded to all four sides of the sterilizer

Answer 31

bio-seal

Question 32

Ways of promoting biosafety

Answer 32

1. Administrative Controls 2. Engineering Controls 3. PPE

Question 33

developing procedures to ensure the work conducted in a way that minimizes the hazard by reducing the duration, frequency or intensity.

Answer 33

Administrative Controls

Question 34

developing or changing policies (SOP, GMT, RM)

Answer 34

Administrative Controls

Question 35

implementing or improving training and education

Answer 35

Administrative Controls

Question 36

enhancing work practices

Answer 36

Administrative Controls

Question 37

ensuring adequate rest breaks

Answer 37

Administrative Controls

Question 38

limiting access to hazardous areas

Answer 38

Administrative Controls

Question 39

Controls developing procedures to ensure the work conducted in a way that minimizes the hazard by reducing the duration, frequency or intensity.

Answer 39

Engineering Controls

Question 40

Ventilation

Answer 40

Engineering Controls

Question 41

Isolation rooms/ negative pressure rooms

Answer 41

Engineering Controls

Question 42

biosafety cabinet

Answer 42

Engineering Controls

Question 43

lab design

Answer 43

Engineering Controls

Question 44

How is BIOSECURITY promoted?

Answer 44

1. Physical security 2. Personnel security 3. Pathogen security 4. Information security 5. Transport security

Question 45

Access control, intrusion detection, alarm assessment

Answer 45

1. Physical security

Question 46

Bg check

Answer 46

Personnel security

Question 47

periodic investigations

Answer 47

Personnel security

Question 48

personnel reliability programme

Answer 48

Personnel security

Question 49

Detailed inventory

Answer 49

Pathogen security

Question 50

acceptance and collection records

Answer 50

Pathogen security

Question 51

inactivation and disposal records

Answer 51

Pathogen security

Question 52

Confidentiality

Answer 52

Information security

Question 53

passwords

Answer 53

Information security

Question 54

back ups

Answer 54

Information security

Question 55

protocols in record keeping

Answer 55

Information security

Question 56

3-way packaging system

Answer 56

Transport security

Question 57

Chain of custody

Answer 57

Transport security

Question 58

International Air Transport Authority (IATA) certified personnel

Answer 58

Transport security

Question 59

Is a written program stating the policies, procedures and responsibilities that protect “workers” from the health hazards associated with the hazardous chemicals used in that particular workplace.

Answer 59

Chemical Hygiene Plan

Question 60

Must be reviewed Anually

Answer 60

Chemical Hygiene Plan

Question 61

1. [?] in relation to safe handle of hazardous chemicals 2. Criteria on what [?] will be implemented for extremely hazardous materials 3. Functioning [?] 4. Availability and location of [?] 5. Identification of [?] for OSHA regulated substances (can’t be exposed in formaldehyde for >8hrs) 6. Designation of [?] responsible for implementing the CHP

Answer 61

SOP PPE and Engineering controls fume hoods and PPE MSDS permissible exposure limits (PELs) personnel

Question 62

Baker's yeast

Answer 62

Level 1

Question 63

Lactobacillus

Answer 63

Level 1

Question 64

Escherichia

Answer 64

Level 1

Question 65

Influenza

Answer 65

Level 2

Question 66

HIV

Answer 66

Level 2

Question 67

Lyme Disease

Answer 67

Level 2

Question 68

Herpes simplex

Answer 68

Level 2

Question 69

Salmonella

Answer 69

Level 2

Question 70

RSV

Answer 70

Level 2

Question 71

Staph

Answer 71

Level 2

Question 72

Strep

Answer 72

Level 2

Question 73

Hepatitis

Answer 73

Level 2

Question 74

TB

Answer 74

Level 3

Question 75

Sars-CoV2

Answer 75

Level 3

Question 76

Yersinia pestis

Answer 76

Level 3

Question 77

Brucella

Answer 77

Level 3

Question 78

S. typhi

Answer 78

Level 3

Question 79

Anthrax

Answer 79

Level 3

Question 80

Histoplasma

Answer 80

Level 3

Question 81

Chlamydia

Answer 81

Level 3

Question 82

Ebola virus

Answer 82

Level 4

Question 83

Small pox

Answer 83

Level 4

Question 84

Marburg

Answer 84

Level 4

Question 85

Lassa

Answer 85

Level 4

Question 86

Arbovirus

Answer 86

Level 4

Question 87

No containment

Answer 87

Level 1

Question 88

Basic teaching and research

Answer 88

Level 1

Question 89

Basic biosafety

Answer 89

Level 2

Question 90

Primary health services

Answer 90

Level 2

Question 91

Diagnostic service and research

Answer 91

Level 2

Question 92

Containment biosafety

Answer 92

Level 3

Question 93

Special diagnostic and research

Answer 93

Level 3

Question 94

Maximum containment

Answer 94

Level 4

Question 95

Dangerous pathogen unit

Answer 95

Level 4

Question 96

Good microbial technique

Answer 96

Level 1

Question 97

Standard precautions

Answer 97

Level 2 Level 3 Level 4

Question 98

Controlled access

Answer 98

Level 3 Level 4

Question 99

directions airflow

Answer 99

Level 3 Level 4

Question 100

airlock entry

Answer 100

Level 4

Question 101

shower exit

Answer 101

Level 4

Question 102

special waste disposal

Answer 102

Level 4

Question 103

None

Answer 103

Level 1

Question 104

Open bench

Answer 104

Level 1 Level 2

Question 105

Biosafety cabinet

Answer 105

Level 2 Level 3

Question 106

other devices

Answer 106

Level 3

Question 107

Class III BSC Class II BSC

Answer 107

Level 4

Question 108

positive pressure suits

Answer 108

Level 4

Question 109

filtered air

Answer 109

Level 4

Question 110

Loss of motivation

Answer 110

Affect the morale and threaten the emotional health of the party involved

Question 111

Promotion of lab safety

Answer 111

Injuries impair the ability to serve

Question 112

Agents: bacteria, parasite, fungi

Answer 112

1. Biological

Question 113

Heat, hazards, noise, vibrations

Answer 113

2. Physical

Question 114

Physical arrangement of the lab

Answer 114

3. Ergonomic

Question 115

Risk of having musculoskeletal diseases

Answer 115

3. Ergonomic

Question 116

Bad posture due to improper microscope use/extraction

Answer 116

3. Ergonomic

Question 117

Chemical Hygiene Plan when exposed to formaldehyde, acid, corrosive substances

Answer 117

4. Chemical

Question 118

Radioactive substances

Answer 118

5. Radiation

Question 119

Affects the mental health

Answer 119

6. Psychological

Question 120

Stress, burnout, no work breaks, shift arrangements, workplace violence

Answer 120

6. Psychological

Question 121

Non-infectious DRY waste

Answer 121

Black

Question 122

Non-infectious WET waste

Answer 122

Green

Question 123

Infectious Pathological Waste

Answer 123

Yellow

Question 124

Chemical waste (heavy metals)

Answer 124

Yellow with Black Band

Question 125

Sharps & pressurized containers

Answer 125

Red

Question 126

Radioactive wastes

Answer 126

Orange

Question 127

Joint Commission (JC)

Answer 127

RACE

Question 128

National Fire Protection Association (NFPA)

Answer 128

PASS

Question 129

RACE:

Answer 129

1. Rescue—anyone in immediate danger 2. Alarm—activate the institutional fire alarm system 3. Contain—close all doors to potentially affected areas 4. Extinguish/Evacuate— extinguish the fire, if possible, or evacuate, closing the door

Question 130

PASS:

Answer 130

1. Pull pin 2. Aim at base of fire 3. Squeeze handles 4. Sweep nozzle, side to side

Question 131

—anyone in immediate danger

Answer 131

1. Rescue

Question 132

—activate the institutional fire alarm system

Answer 132

2. Alarm

Question 133

—close all doors to potentially affected areas

Answer 133

3. Contain

Question 134

— extinguish the fire, if possible, or evacuate, closing the door

Answer 134

4. Extinguish/Evacuate

Question 135

Wood, paper, or clothing

Answer 135

Class A

Question 136

Water

Answer 136

Class A

Question 137

Class A

Answer 137

Class A

Question 138

Flammable organic chemicals

Answer 138

Class B

Question 139

Class B

Answer 139

Class B

Question 140

Dry chemicals, carbon dioxide, foam, or Halon

Answer 140

Class B

Question 141

Electrical

Answer 141

Class C

Question 142

Class C

Answer 142

Class C

Question 143

Dry chemicals, carbon dioxide, or Halon

Answer 143

Class C

Question 144

Combustible metals

Answer 144

Class D

Question 145

None

Answer 145

Class D

Question 146

Sand or dry powder

Answer 146

Class D

Question 147

Class ABC

Answer 147

Class D

Question 148

Dry chemicals

Answer 148

Class D

Question 149

Grease, oils, fats

Answer 149

Class K

Question 150

Class K

Answer 150

Class K

Question 151

Liquid designed to prevent splashing and cool the fire.

Answer 151

Class K

Question 152

Non-negotiable; should always be followed

Answer 152

Standard Precautions

Question 153

– hazard identification

Answer 153

Risk assessment

Question 154

– policies and management decisions on risk

Answer 154

Risk management

Question 155

– exchange of information involving risk

Answer 155

Risk communication

Question 156

Foundation for biosecurity

Answer 156

Biosafety

Question 157

Completely shut

Answer 157

bio-seal

Question 158

– only way for an equipment to exit the room

Answer 158

Autoclave

Question 159

Aka ―SOP‖ for chemicals

Answer 159

Chemical Hygiene Plan

Question 160

(to check integrity of a person)

Answer 160

personnel reliability programme

Question 161

(monitoring of specimen, etc.)

Answer 161

Detailed inventory

Question 162

Physically remove the hazard

Answer 162

Elimination

Question 163

Replace the hazard

Answer 163

Substitution

Question 164

Isolate people from the hazard

Answer 164

Engineering Controls

Question 165

Change the way people work

Answer 165

Administrative Controls

Question 166

Protect the worker

Answer 166

Personal Protective Equipment (PPE)