Lesson 6

Question 1

Are growth of microorganism on a culture medium

Answer 1

Cultures

Question 2

Is a liquid, semi-solid or solid medium utilized to observe growth patterns of microorganism as well as for transport and storage.

Answer 2

Culture Medium

Question 3

Culture composed of only one specie

Answer 3

Pure Culture

Question 4

Culture composed of more than one specie

Answer 4

Mixed Culture

Question 5

– Is composed of several species
contained in a separate culture
medium (one species/culture
medium).
– It is used for academic and
industrial purposes.

Answer 5

Stock Culture

Question 6

Colonies may be described as being
large, medium, small, or pinpoint.

Answer 6

Size

Question 7

Refers to the edge of colonies

Answer 7

Form or Margin

Question 8

■ Determined by tilting culture plate and looking at the
side of the colony
■ Raised, convex, flat, umbilicate, or umbonate

Answer 8

Elevation

Question 9

May be described as transparent, translucent, opaque.

Answer 9

Density

Question 10

Used to describe a particular colony genus in genera

Answer 10

Color

Question 11

Determined by touching a colony with a sterile loop

Answer 11

Consistency

Question 12

Inherent characteristic of specific organisms

Answer 12

Pigment

Question 13

What is the distinctive odor of staphylococcus aureus?

Answer 13

Old Sock

Question 14

Vinelike growth in media

Answer 14

Streamers

Question 15

Overall cloudiness of liquid

Answer 15

Turbidity

Question 16

It contains 0% agar

Answer 16

Liquid Medium

Question 17

It contains 0.5 to 1% agar

Answer 17

Semi-Solid Medium

Question 18

It contains 2-3% agar

Answer 18

Solid Medium

Question 19

Agar will melt at what temperature?

Answer 19

80 to 90°C

Question 20

Agar will solidify at what temperature?

Answer 20

40 to 50°C

Question 21

Cooling temp for distribution of agar into plates

Answer 21

55-60°C

Question 22

• It is a medium where all the
components are known to the
user.
• It is use for research purposes.

Answer 22

Synthetic or Defined Medium

Question 23

• It is composed of some unknown
substances (peptones, meat and
yeast extracts).
• It is very useful for isolation of
bacteria.
• examples: nutrient broth, TSB
and MacConkey agar

Answer 23

Non-Synthetic or Complex Medium

Question 24

• It used for obligate intracellular
bacteria (Rickettsia, Chlamydia).
• Examples: W138, HELA 229 cells
and Mc Coy cells

Answer 24

Tissue Culture Medium

Question 25

Are routinely used in the laboratory and without added supplement

Answer 25

1. Simple Media/General Purpose Media/Supportive Media

Question 26

■ It is used to propagate the growth of certain group of organisms. ■ They contain specific nutrients; liquid-type media. ■ It can also be used as supplement to agar plates. ■ Examples: Alkaline Peptone Water, Selenite F, Thioglycolate, tetrathionate, Gram- negative (GN) broth, Lim broth (group B streptococci)

Answer 26

Enrichment Broth

Question 27

■ These are media with added supplements such as blood, vitamins and yeast extract, necessary for the growth of fastidious organisms. ■ These are solid-type media

Answer 27

Enriched Media/Nonselective Media

Question 28

Standard nonselective culture medium

Answer 28

BAP

Question 29

Has horse blood is used for the isolation of Hemophilus

Answer 29

CAP

Question 30

These are media that allow visualization of metabolic differences between groups of bacteria. ■ Examples: MacConkey, BAP, EMB, and HEA

Answer 30

Differential Media

Question 31

"Greenish” zone of hemolysis

Answer 31

ALPHA

Question 32

“Clear” zone of hemolysis

Answer 32

Beta

Question 33

Means No Hemolysis

Answer 33

Gamma

Question 34

These are media incorporated with antibiotics, dyes or chemicals to inhibit the growth of other organisms while promoting the growth of the desired organism.

Answer 34

Selective Media

Question 35

contains bile salt and dyes (acid fuchsin) for non-enteric pathogens; it inhibits the normal flora of the lower GIT.

Answer 35

HE Agar

Question 36

It contains 0.25% Sodium Deoxycholate salt and sodium thiosulfate to differentiate Salmonella (red with black centers) from Shigella (red or clear colonies).

Answer 36

XLD Agar

Question 37

Inhibitory to Gram-positive Bacteria

Answer 37

1. Crystal/Gentian Violet 2. Basic Fuchsin 3. Bile Salt

Question 38

Inhibitory to Gram-negative Bacteria

Answer 38

1. Potassium Tellurite 2. Sodium Azide

Question 39

Inhibitory to Swarming Bacteria

Answer 39

1. Alcohol 2. Chloral Hydrate

Question 40

Is used to isolate bacteria with specific growth requirements.

Answer 40

Special Media