Lesson 9: Handling and Processing of Blood Specimens for Laboratory Testing

Question 1

Result of a test is compromised when those are not followed during pre-analytical phase:

Answer 1

*collection
procedures
*storage
*processing
*transporting
protocol

Question 2

Laboraty error /mishandling

Answer 2

approximately
48% to 68%

Question 3

Routine Handling

Answer 3

✓ Mixing tubes by inversion
usually between 3 to 10
✓ transported with the stopper

Question 4

Specimens should be transported with the stopper to:

Answer 4

1)avoid contact between
contents and the stopper
2)minimize agitation
3)aid clot formation

Question 5

Needed when handling blood specimen to protect its condition and quality

Answer 5

SPECIAL CARE/HANDLING

Question 6

What should be the temperature of the specimen when being transported?

Answer 6

near body temperature
which is 37˚C.

Question 7

Specimens are Pre-warmed at what temperature?

Answer 7

37°C

Question 8

Hold the temperature for 15 minutes from
removal from the incubator.

Answer 8

PORTABLE HEAT BLOCKS

Question 9

It can be used for specimen that can withstand a
temperature slightly higher than 37˚C

Answer 9

HEEL WARMER

Question 10

Specimens that can withstand slightly higher than 37°C

Answer 10

█Cold agglutinin
█Cyrofibrinogen
█Cyroglobulins

Question 11

Slows the metabolic process

Answer 11

CHILLING

Question 12

immediately tested or refrigerated if
needed

Answer 12

Chilled Specimens

Question 13

Chilled specimens should be submerged in:

Answer 13

CRUSHED ICE AND
WATER SLURRY

Question 14

Chilled Specimens

Answer 14

█ Acetone
█ Ammonia
█ Free Fatty Acids
█ Gastrin
█ Glucagon
█ Pyruvate

Question 15

Exposure to light could affect the result of
a specimen

Answer 15

Light-sensitive specimens

Question 16

Used in light sensitive specimens:

Answer 16

✓ALUMINUM FOIL
✓LIGHT-BLOCKING
AMBER-COLORED CONTAINER

Question 17

Light-sensitive specimens

Answer 17

█ Bilirubin
█ Carotene
█ Red Cell Folate
█ Serum Folate
█ Vitamin B2
█ Vitamin B6

Question 18

CRITERIA FOR
SPECIMEN REJECTION

Answer 18

• not identified properly
• inadequate volume
• hemolysis
• wrong tube
• incorrect collection time
• exposure to light
• did not follow testing time
  limits
• delay or error in
  processing

Question 19

Specimen should:

Answer 19

✓identified
✓logged or accessioned
✓sorted by department and
type of processing
✓evaluated for specimen
suitability which is
necessary for accurate
reasons

Question 20

QNS

Answer 20

Quantity not sufficient

Question 21

Routine blood specimen should be transported in the Laboraty within how many minutes?

Answer 21

Within 45 mins.

Question 22

How long is the centrifugation?

Answer 22

1 hour

Question 23

It should not be centrifuged

Answer 23

Hematology specimens with EDTA

Question 24

priority in terms of
transportation, processing
and testing

Answer 24

“STAT” or
“emergency”

Question 25

Blood Smear from EDTA Specimen

Answer 25

prepared 1 hour from collection

Question 26

EDTA specimen for CBC

Answer 26

analyzed within 6 hours but is stable 24 hours from collection if stored at room temperature

Question 27

EDTA specimen for erythrocyte sedimentation rate (ESR) determination

Answer 27

tested 4 hours (room temperature) and 12 hours (refrigerated)

Question 28

EDTA specimen for reticulocyte counts

Answer 28

stable for 6 hours (Room Temperature) and 72 hours if refrigerated

Question 29

Glucose test drawn in sodium fluoride tubes

Answer 29

stable for 24 hours (room temperature) but stable for up to 48 hours when refrigerated at 2 °C to 8 °C

Question 30

Prothrombin time (PT)

Answer 30

stable for 24 hours and partial thromboplastin time (PTT) should be analyzed within 4 hours from collection

Question 31

What components does centrifuge separates?

Answer 31

cells, plasma or serum of blood specimen

Question 32

STOPPERS

Answer 32

✓avoid contamination ✓evaporation ✓aerosol formation ✓pH changes

Question 33

CENTRIFUGATION

Answer 33

✓tubes should be BALANCED ✓same size and volume of specimen

Question 34

PLASMA SPECIMEN (anticoagulants)

Answer 34

centrifuged immediately

Question 35

SERUM SPECIMEN (completely clotted)

Answer 35

30 to 60 minutes at room temp

Question 36

cover the stopper to catch drops of blood that may leak or to catch aerosol

Answer 36

GAUZE or TISSUE

Question 37

portion of a sample specimen taken for chemical analysis or testing

Answer 37

ALIQUOT

Question 38

Aliquot is prepared when:

Answer 38

MULTIPLE TESTS ordered on a single specimen

Question 39

ALIQUOT PREP

Answer 39

✓ do not put specimen with different anticoagulants in the same aliquot tube ✓ cover the tube as soon as it is filled

Question 40

OSHA-REQUIRED

Answer 40

protective equipment worn when processing specimen

Question 41

OSHA

Answer 41

OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION

Question 42

Protective Equipments required

Answer 42

✓ gloves ✓ laboratory gown ✓ laboratory coats ✓ masks

Question 43

An Act Strengthening Compliance with Occupational Safety and Health Standards

Answer 43

RA 11058