LI Bacterial

Question 1

Food poisoning vs. Food-Associated Illness

Answer 1

Poisoning = Toxin ingestion with fast onset
FAI = Ingest organism, longer onset

Question 2

Bugs that cause Food poisoning

Answer 2

S Aureus
B. Cerus
C Botulinum

Question 3

Gram + Bacili
Spore Forming
Facultative Anaerobe
Motile

Answer 3

Bacillus Cerus

Question 4

Spores that can survive RICE COOKING

Answer 4

Bacillus Cerus

Question 5

B cerus toxin that causes vomiting?
Mechanism?
Pre-formed?

Answer 5

Cerulide

• Forms pores
• Pre-formed

Question 6

B Cerus toxin that causes diarrhea?
Mechanism?
Pre-formed

Answer 6

Large MW Enterotoxin

• Increases fluid excretion
• NOT pre-formed

Question 7

Gram + Bacili
Spore Forming
Obligate Anaerobe
Motile

Answer 7

Clostridium botulinum

Question 8

Gram + Bacili
Spore Forming
Obligate Anaerobe
Non-Motile

Answer 8

Clostridium perfringens

Question 9

C. perfringens specific symptoms

Answer 9

Diarrhea WITHOUT vomiting or fever

Gas Gangrene

Question 10

Gram + Bacili
NON-Spore Forming
Facultative Anaerobe
Motile

Answer 10

Listeria monocytogenes

Question 11

Diagnosing listeria?

Answer 11

Culture sterile site, NOT stool

Question 12

Listeria complications?

Answer 12

Meningitis

Sepsis

Question 13

Most common cause of foodborne DEATH?

Answer 13

Listeria

Question 14

Gram -
Oxidase +
Microaerophilic
Cold Sensitive

Answer 14

Camplobacter Jejuni

Question 15

Camplobacter reservoir?

Answer 15

Poultry

Question 16

Camplobacter mechanism of disease?

Answer 16

Cell invasion triggering an inflammation response

Question 17

Camplobacter grows on what agar?

Answer 17

Karmali

Question 18

Complication of Camplobacter?

Answer 18

Guillan-Barre

Question 19

Gram -
Comma shaped
Oxidase +
Facultative Anaerobe
Flagellated
Motile

Answer 19

Vibrio species

Question 20

Bloody diarrhea, cramps and fever lasting less than one week?

Answer 20

V hemolytica

Question 21

V hemolytica toxin and mechanism?

Answer 21

Hemolysin causes RBC and intestinal epithelial cell lysis

Question 22

V hemolytica reservoir?

Answer 22

Shellfish, oyster, seafood

Question 23

Gram -
Rod
Lactose +
Facultative Anaerobe
Motile

Answer 23

E coli

Question 24

EHEC vs. ETEC

Answer 24

EHEC = hemorrhagic due to shiga-like toxin
ETEC = toxigenic due increased cAMP increasing H2O secretion

Question 25

Which E coli type has a quicker incubation?

Answer 25

EHEC (1-3 days)

Question 26

Which E coli type does NOT penetrate cells?

Answer 26

ETEC

Question 27

Food-Borne organisms?

Answer 27

``` C perfringens L monocytogenes V parahemolytica C jejuni Sal. enterica ```

Question 28

``` Gram - Lactose - Facultative Anaerobe H2S + Motile ```

Answer 28

Salmonella species

Question 29

``` Gram - Lactose - Facultative Anaerobe H2S - Non - Motile ```

Answer 29

Shigella species

Question 30

What color with salmonella and shigella species be on SS agar?

Answer 30

``` Salmonella = Black (H2S+) Shigella = Clear (H2S-) ``` Neither will be red (Lactase +)

Question 31

Sal. enterica mechanism?

Answer 31

Intracellular replication | Closure of ion channels causes cell lysis

Question 32

Shigella mechanism?

Answer 32

Enters GI via M Cells Infects macrophages Spread to GI epithelial cells via T3SS

Question 33

Organisms that can cause HUS?

Answer 33

EHEC/ETEC | Shigella

Question 34

Bloody diarrhea causes?

Answer 34

Shigella EHEC V. parahemolytica

Question 35

Mildest shigella infection?

Answer 35

S sonnei

Question 36

Cause of bacillary dysentery?

Answer 36

S flexnori | S dysenteriae

Question 37

Most severe shigella species? Why?

Answer 37

S dysenteriae | - Shiga toxin produced (vascular endothelial damage)

Question 38

GI bugs that can cause reactive arthritis?

Answer 38

Shigella flexnori | Salmonella enterica

Question 39

V cholera mechanism and symptoms?

Answer 39

Increased cAMP "rice-water" stool Dry membranes

Question 40

Reservoir for Salmonella typhi?

Answer 40

Only humans

Question 41

What bacteria is there a vaccine available for?

Answer 41

S. typhi