Lineweaver Burk plot Flashcards
(5 cards)
What is the LB plot
double reciprocal plot of the michaelis menten plot
what are the limitations of the MM plot
using MM graph makes the determination of vmax and km inaccurate due to hyperbolic plot
vmax is approached but not attained
what is the effect of a competitive inhibitor on the michaelis menten plot and LB plot
vmax is unaffected, km is increased.
no effect on the vmax value bc the addition of more substrate can overcome the effect of the comp inhibitor
km increases as there is increased competition between the substrate and the comp inhibitor
what is the effect of a noncompetitive inhibitor on an LB plot and MM plot
vmax is lowered, km is unaffected
vmax is lowered bc the enzyme substrate inhibitor complex is unable to form any product
km is unaffected bc the enzyme substrate complex will form bc the inhibitor will bind to the allosteric site
What is the effect of pH on enzymes?
enzyme tertiary structure held together in part by ionic bonds
at high/low pH ionic bonds become disrupted = change structure
