Long Quiz (1-10) Flashcards
Photolithotropic bacteria
Chromatium okenii
Photoorganotroph –
Rhodospirillum rubrum
Either chemolithotrophs or chemoorganotrophs –
Pseudomonas pseudoflava
Chemolithotropic heterotrophs-
Desulfovibrio desulfuricans
Bacterium that causes leprosy –
Mycobacterium leprae
Differential-
blood containing agar medium
Selective media-
cellulose, antibiotics
Useful for cultivating microaerophilic bacteria-
semisolid media
Bacteria that has an optimum pH of 2.0-3.5 and can grow in a range between pH 0.5 and 6.0 –
Thiobacillus thiooxidans
The cells are lined side by side like matchsticks –
Corynebacterium diphtheria
Disks arranged like stacks of coins –
caryophanon
Bacteria that has gliding motility-
Cytophaga spp.
They help to protect bacteria from thermal injury –
teichoic acid
Can be easily destroyed by treatment of an enzyme called lysozyme-
gram positive
Dormant, thick walled, dessication resistant forms that develop by differentiation of a vegetative cell and which can later germinate under suitable conditions –
cysts
Bacteria that has monotrichous flagellar arrangement –
Pseudomonas aeruginosa
Bacteria that has peritrichous flagellar arrangement –
Salmonella typhi
Prosthecate bacterium that undergoes transverse binary fission –
Prosthecobacterium fusiformis
Bacteria that reproduce by budding –
Rhodopseudonomonas acidophila
Used to measure viable cells –
plate count method, membrane filter method
Medium containing crystal violet dye-
gram negative intestinal bacteria
MacConkey agar –
gram negative intestinal pathogens (shigella, salmonella)
Selected when mixed culture can be heated to 80’c for 10 mins –
endospore forming bacteria
Bacteria selected when cultures are incubated at 0 to 5’c –
psychrophilic bacteria
Bacteria selected when cultures are incubated at 55’c –
thermophiles
Alkali tolerant organisms (high pH) –
Vibrio cholera
Extremely susceptible to S. pneumoniae –
mice
This manipulation thins out the bacteria on agar surface-
streak plate technique
Used for isolation of stringent anaerobes-
roll tube technique
Methods of maintenance and preservation of pure cultures
Periodic Transfer to fresh media, Ovelying cultures with mineral oil, Lyophilization, Storage at low temperature
Smooth colonies:
Streptococcus pneumoniae, salmonella
Rough colony:
Mycobacterium tuberculosis
Serratia marcescens-
red
Blue water soluble pigment –
pyocyanin
Sparingly water soluble-
chlororaphin
Coenzyme of thiamine –
cocarboxylase
Low molecular weight organic molecule-
coenzyme
Fluorescent pigment-
pyoverdin
Sequences of reactions performed by enzymes-
pathways
Coenzyme of niacin-
nicotinamide adenine dinucleotide