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M1 - LAB Flashcards

1
Q

ELECTROPHORESIS SEPERATION BASED ON

A

ELECTRICAL CHARGE DENSITY

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2
Q

MIGRATION OF CHARGED SOLUTES IN A MEDIUM UNDER ELECTRIC FIELD

A

ELECTROPHORESIS

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3
Q

ELECTROPHORESIS BUFFER

A

BARBITAL (PH 8.6)

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4
Q

MOLECULE WHOSE NET CHARGE CAN EITHER BE + OR -

A

AMPHOLYTE

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5
Q

IN ELECTROPHORESIS ___ ATTRACTS

A

OPPOSITE

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6
Q

MIGRATION OF SMALL IONS

A

IONTOPHORESIS

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7
Q

MIGGRATION OF CHARGED MACROMOLECULE IN POROUS MEDIUM

A

ZONE ELECTROPHPORESIS

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8
Q

ZE: SEPERATION BY MOLECULAR SIZE

A

CELLULOSE ACETATE

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9
Q

ZE: SEPERATION BY ELECTRICAL CHARGE

A

AGAROSE GEL

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10
Q

ZE: SEPERATION BY CHARGE AND MOLECULAR SIZE

A

POLYCRILAMIDE GEL

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11
Q

POLYCRILAMIDE GEL SEPERATES PROTEIN INTO __

A

20 FRACTIONS

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12
Q

DISPLAY OF PROTEIN ZONES

A

ELECTROPHORETOGRAM

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13
Q

FACTORS AFFECTING MIGRATION

A

TYPE OF SUPPORT MEDIUM
TEMP OF SUPPORT MEDIUM

MW OF PARTICLE
STRENGTH OF E. FIELF

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14
Q

THE DRIVING FORCE OF ELECTRO

A

POWER SUPPLY

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15
Q

FIVE COMPONENTS

A

POWER SUPPLY
SUPPORT MEDIUM
BUFFER
SAMPLE
DETECTING SYSTEM

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16
Q

ELECTROPHORESIS SEPERATES:

A

PROTEIN
PESTICIDE
DRUGS
AMINO ACIDS
NUCLEIC ACIDS

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17
Q

USES FILTER PAPER, ECONOMICAL

FOR LOW MW

A

PAPER ELECTROPHORESIS

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18
Q

PAPER ELECTRO SEPERATION TIME

A

12-14HRS

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19
Q

WHAT CANT BE RESOLVED BY PAPER E.

A

PROTEIN
HYDROPHILIC MOLECULES

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20
Q

TAKES - CHARGE FORM ADSORPTION OF HYDROXYL IONS

A

SUPPORT MEDIA

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21
Q

PAPER E. SUPPORT MEDIA: GREATER RESO, SOAKED IN WATER FILLED WITH ELECTROLYTES

A

CELLULOSE PAPER

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22
Q

CELLULOSE PAPER AIR SPACE

A

80%

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23
Q

PAPER E. SUPPORT MEDIA: USED AS PURIFIED FRACTION OF AGAR

NEUTRAL

A

AGAROSE GEL

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24
Q

MOVEMENT OF BUFFER ION AND SOLVENT RELATIVE TO FIXED SUPPORT

A

ELECTROENDOSMOSIS

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25
AMOUNT NEEDED IN ELECTROENDOSMOSIS
2ML
26
PAPER E. SUPPORT MEDIA: SEPERATES PROTEINS ON SURFACE CHARGE AND MOL SIZE NOT WIDELY USED
STARCH
27
PAPER E. SUPPORT MEDIA: SEPERATION BASED ON CHARGE AND MOL SIZE STUDY INDIV PROTEINS
POLYACRYLAMIDE GEL
28
SEPERATION THROUGH MOLECULAR SIEVEING TECHNIQUE
GEL ELECTROPHORESIS
29
PROTEINS ARE SEPERATED BASED ON CHARGE TO MASS RATIO AND MOL SIZE
MOLECULAR SIEVING PHENOMENON
30
MOLECULAR SIEVING PHENOMENON BASIS (2)
CHARGE TO MASS RATIO MOLECULAR SIZE
31
E. GEL APARATUS: THICKNESS LIMITED, ONE GEL PER APARATUS
HORIZONTAL
32
E. GEL APARATUS: DIFF THICKNESS, MORE THAN ONE GEL PER APARATUS
VERTICAL
33
ELECTROPHORESIS PROTEIN STAIN
AMIDO BLACK BROMPHENOL BLUE NIGROSIN
34
ISOENZYMES USES WHAT STAIN
NITROTETRAZOLEUM BLUE
35
PROTEIN STAIN DARK BLUE BAND
AMIDO BLACK
36
PROTEIN STAIN GIVING RED BAND
PONCEAU S DYE
37
LIPOPROTEIN STAINS
OIL RED O SUDAN BLACK B
38
MODIFICATION OF ELECTROPHORESIS PROTEINS ARE SEPERATED BASED ON ISOELECTIC PH
ISOELECTRIC FOCUSING
39
ISOELECTRIC FOCUSING MEDIA (3)
AGAROSE GEL POLYACRILAMIDE GEL CELLULOSE ACETATE
40
ACIDIC GROUP > BASIC GROUP = LOW PH
ACIDIC PROTEIN
41
BASIC > ACIDIC = HIGH ISOELECTRIC PH
BASIC PROTEIN
42
ISOLECTRIC PH RANGE
3-12
43
MIXTURE OF MOLECULES CONTAINING MULTIPLE ALIPHATIC AND AMINO CARBOXYLATE GROUPS
CARRIER AMPHOLYTES
44
IEF MEANS
ISOELECTRIC FOCUSING
45
DERIVATIVE OF ACRYLAMIDE (HAS DOUBLE BOND AND BUFFERING GROUPS )
ACRYLAMIDE BUFFER
46
GEL FOR ISOELECTRIC FOCUSING POLYACRYLAMIDE IS POLYMERIZED WITH __
RIBOFLAVIN
47
AUTOMATION ADVANTAGES (#)
RAPID RESULT ECONOMICAL ERRORS IN CALCULATIONS REDUC4ED
48
1ST AUTOMATED ANALYZER YEAR
1957
49
FIRST ANALYZER BY
TECHNICON
50
BATCH ANALYZER IN 1957
CONTINOUS FLOW SINGLE CHANNEL
51
CONTINOUS FLOW SINGLE CHANNEL CAN HANDLE __ SAMPLES/HOUR
40
52
NEXT ANALYZER TO BE DEVELOPED
SIMULTANEOUS MULTIPLE ANALYZER SMA 6, SMA 12
53
SMA ___ RESULTS SIMULT AT RATE OF ___ PER HOUR
6-12, 360-720
54
SPECIALTY AREA W RAPIDLY DEVELOPING ARSENAL OF ANALYZERS
IMMUNOCHEMISTRY
55
LIQUIDS ARE PUMPED INTO SYSTEM OF CONTINOUS TUBING
CONTINOUS FLOW
56
CONTINOUS FLOW SEPERATING AND CLEANING MEDIA
AIR BUBBLES AT REGULAR INTERVAL
57
USES FORCE BY CENTRIFUGATION
CENTRIFUGAL ANALYSIS
58
MOST POPULAR - SEPERATION OF EACH SAMPLE INTO CONTAINER - CAN GO STAT
DISCRETE ANALYSIS
59
WHEN ATOMS RETURN TO GROUND STATE, THEY EMIT RADIATION
FLAME EMISSION
60
IF YOU PASS BY THIS, STUDY PICTURES, PASADAHAN AND PANGALANAN MO
OK

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