M11 Gram (-ve part 2)

Question 1

list three gram negative anaerobic bacilli

Answer 1

tennerella
fusobacterium
leptotrichia

Question 2

which saccharolytic, non-pigmented bacteria pits the surface of blood agar?

Answer 2

tannerella forsythia

Question 3

which condition is a tanneralla forsythia an important part of?

Answer 3

periodontitis

Question 4

which important virulence factors are associated with tannerella forsythia?

Answer 4

fibronectin/ fibrinogen binding BspA

Protease

Question 5

what are the effects of BspA on tanerella forsythia?

Answer 5

adhesion
Induces release of bone reabsorbing pro-inflammatory cytokines
induces chemokines (recruits neutrophils, inflammation)

Question 6

what are the interactions of BspA (Tannerella forsythia?

Answer 6

P.gingivalis enhances attachement to epithelial cells

- forms mixes films with fusobacterium

Question 7

which GNAB is mainly found in human gingival sulcus?

Answer 7

fusobacterium nucleatum

Question 8

which bacteria can adhere with Gm +ve and Gm -ve?

Answer 8

fusobacterium nucleatum

Question 9

which virulence factor is gaining a lot of attention in extra-oral infections? (fusobacterium)

Answer 9

FadA

Question 10

the spirochaete combination of Fusobacterium nucleatum and Treponema vincentii is?

Answer 10

fusospirochaetal infection

Question 11

what is fusobacterium nucleatum associated with?

Answer 11

Acute ulcerative Gingivitis (AUG)

Question 12

how does fusobacterium (mainly smokers) lead to AUG

Answer 12

• induces protein kinase (cell survival)
• stimulates collagenase 3 expression
• secretes serine protease
• immune suppression

Question 13

which saccrolytic Gnab makes lactic acid as a major fermentation product?

Answer 13

leptotrichia -

Question 14

which main species is preferentially isolated from plaque overlying caries?

Answer 14

leptotrichia buccalis (present in dental plaque_
- extra oral infections in seriously ill patients

Question 15

What is the meaning of gram -ve facultative anaerobes?

Answer 15

Prefer oxygen and are often capnophilic

Question 16

what are the 4 main oral species of facultative anaerobes?

Answer 16

Hemophilus
Aggregatibacter
Eikenella
capnocytophaga

Question 17

which gram -ve small bacilli are found in the mouth in first few days of birth?

Answer 17

haemophilus

Question 18

what is the most common haemophilus soecies found in supragingival plaque?

Answer 18

haemophilus segnis

Question 19

describe aggregatibacter oral species

Answer 19

gram -ve
capnophilic
periodontipathogen
often found associated with actinomyces

Question 20

what is juvenile periodontitis?

Answer 20

common in girls puberty
west african and asian fam
localised JP (first permanent molars and incisors)

Question 21

which actinomyces is associated with Juvenile periodontitis?

Answer 21

A.actinomycetemcomitans

rapid destructive periodontitis

Question 22

what anitbiotic is given for A.actinomycetemcomitans?

Answer 22

tertracycline and beta lactam

Question 23

describe eikenella

Answer 23

gram -ve asaccharolytic small bacillus that pits or corrodes agar

Question 24

what serious extra oral infection can eikenella cause?

Answer 24

brain and abdominal abscesses, endocarditis 25% of human bite/clench fist injuries

Question 25

which curved gram -ve bacilli is found in plaque and periodontal pockets?

Answer 25

Campylobacter rectus

Question 26

what is the red complex?

Answer 26

tennerella forsythia porphyromonas gingivalis treponema denticola

Question 27

what is the orange complex made up of?

Answer 27

prevotella intermedia prevotella nigrescens fusobacterium nucleatum