M3:U4 Leukocyte Esterase Nitrite Ascorbic Acid

Question 1

Type of uti caused by enteric gram-negative organisms moving from urethra to urinary bladder

Answer 1

Ascending infections

Question 2

Causative agents of ascending infection

Answer 2

Escherichia coli
Proteus species
Enterobacter
Klebsiella

Question 3

Lower urinary tract infection

Answer 3

Urethritis
cystitis

Question 4

Upper urinary tract infection

Answer 4

pyelonephritis

Question 5

Normal amount of leukocytes in urine

Answer 5

0-8 HPF
10 WBC/uL

Question 6

Found in azurophilic granules of granulocytes

Answer 6

Leukocyte esterase

Question 7

Factors to consider when using nitrite test

Answer 7

Infecting bacteria is nitrate reducer
adequate time to let bacteria reduce nitrate (4 hrs)
availability of dietary nitrate subsequent conversion to nitrogen in an hour

Question 8

Wbc that does not produce leukocyte esterase

Answer 8

Lymphocyte

Question 9

Which is better nitrite or leukocyte esterase

Answer 9

Leukocyte esterase

Question 10

Also known as the 11th reagent pad

Answer 10

Ascorbic acid

Question 11

Analytes affected by ascorbic acid

Answer 11

Blood
bilirubin
glucose
nitrite

Question 12

Strong reducing substance that forms colorless compound leading to false negative results

Answer 12

Ascorbic acid