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Flashcards in M6 Deck (14)
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1
Q

The function of the dideoxy (dd) nucleotides that are used in the Sanger method of DNA sequencing is to

A

Incorporate into newly replicated DNA strands and terminate their synthesis

2
Q

The use of an antibiotic-resistance gene on a plasmid used in genetic engineering makes

A

Direct selection possible

3
Q

The following steps must be performed to make a bacterium produce human protein

A
Eukaryotic transcription
Reverse transcription
Restriction enzyme
DNA ligase
Transformation
Prokaryotic transcription
Translation
4
Q

Southern blotting is used to detect a specific

A

DNA.

5
Q

In the large-scale production of a particular human protein in E. coli cells, the cDNA corresponding to the protein was modified so that the expressed protein would have six histidine residues at the C-terminus. The purpose of this modification was

A

to facilitate purification of the expressed protein though binding to an affinity column containing chelated nickel.

6
Q

What is one of the advantages of producing human growth hormone (HGH) from a cloned gene in a bacterial cell?

A

Larger quantities are available

7
Q

The restriction enzyme EcoRI cuts between the G and the A of the sequence GAATTC. Which statement is true about this enzyme?

A

It leaves sticky ends of sequence AATT.

8
Q

Which choice is an example of “recombinant DNA”?

A

a bacterial plasmid combined with a human gene

9
Q

What is a clone?

A

an exact copy of a DNA, cell or organism

10
Q

Microarray technology is a process in which:

A

genes active within tissues are identified using a complementary DNA probe.

11
Q

A “transgenic” animal

A

contains a foreign gene

12
Q

What is the advantage of producing human factor IX in sheep rather than in bacteria?

A

Sheep will produce recombinant protein that is chemically more similar to humans.

13
Q

Which of the following best describes the complete sequence of steps occurring during EVERY cycle of PCR?

A

The mixture is heated to a high temperature to denature the double-stranded target DNA
The primers hybridize to the target DNA
DNA polymerase extends the primers to make a copy of the target DNA

14
Q

In animal cloning why is an egg cell required?

A

Egg proteins are required for embryonic development