M7

Question 1

Which of the following patients could have diabetes based on the diagnostic criteria?
A patient with an HBA1c result of 4.0%.
A patient with a 2-HPPG of 185 mg/dL
A patient with an FBS level of 200 mg/dL
A patient with an RBS result of 150 mg/dL

Answer 1

A patient with an RBS result of 150 mg/dL

Question 2

Which of the following can be used to preserve glucose?
EDTA
Fluoride
Citrate
Oxalate

Answer 2

-Fluoride

Question 3

What is the enzyme needed for the reaction below?
Glucose + O2 → Gluconic acid + H2O2
Hexokinase
Glucosamine
Amino aspartate
Glucose oxidase

Answer 3

-Glucose oxidase

Question 4

Which of the following methods for glucose determination utilizes reverse photometry by monitoring the decrease in absorbance?
Hagedorn-Jensen
Polarographic GOD method
Hexokinase method
O-toluidine method

Answer 4

Hagedorn-Jensen

Question 5

Bernard has been recently experiencing the symptoms of overeating, frequent thirst and urination. His doctor requested to a laboratory to have his random blood glucose tested. The results showed a value of 215 mg/dL, presenting a rather high level. Which of the following would confirm that he has DM if his physician were to request another test the next day?
All of the answers are correct.
HbA1c of 6%
Fasting plasma glucose of 7.3 mmol/L
OGTT 2hr post-load glucose value of 118 mg/dL

Answer 5

Fasting plasma glucose of 7.3 mmol/L

Question 6

What term is used when we loosen the clot after centrifugation?
Precipitation
Deproteinization
Agglutination
Ringing

Answer 6

-Ringing

Question 7

Which of the following is the reference range of Random blood glucose?
45-130 mg/dL
150-200 mg/dL
30 - 200 mg/dL
50-110 mg/dL

Answer 7

-45-130 mg/dL

Question 8

in the GOD-PAP enzymatic method for glucose measurement, in the presence of Glucose oxidase, glucose is oxidized to which of the following?
Acetaldehyde and water
Quinoneimine and water
Gluconic acid and hydrogen peroxide
Glucose-6-phosphate and ADP

Answer 8

Gluconic acid and hydrogen peroxide

Question 9

Which of the following statements is true about Diabetes Mellitus Type 1?
1. There is an absolute insulin deficiency
2. Destruction of a cells of the pancreas
3. Ketosis is common
4. It only occurs during childhood
2 and 4
1, 2, and 3
1 and 3
1,2,3 and 4

Answer 9

-1 and 3

Question 10

Which of the following statements is incorrect?
1. Greater amounts of glucose consumed during the reaction in the polarographic glucose oxidase method are equivalent to lower amounts of glucose in the sample.
2. Catalase and molybdate can be added to the sample to promote production of oxygen from hydrogen peroxide in a polarographic GOD method.
3. In a glucose oxidase coupled reaction, ascorbic acid may cause the glucose level to rise.
4. Glucose oxidase catalyzes the oxidation of glucose from gluconic acid and hydrogen peroxide.
2,3,4
1,2,3
1,2,3,4
1,3,4

Answer 10

1,2,3,4

Question 11

Which of the following is/are glycolytic inhibitor/s?
1. Fluoride
2. Oxalate
3. Monoiodacetate
4. Mannose
5. Citrate
I,III & IV
I, II & IV
I, II, III & IV
II, III & V

Answer 11

-I,III & IV

Question 12

Lowella, a known diabetic was requested by her physician to have her 2-hour postprandial blood sugar done. She is keen on taking her maintenance medications & follow-up checkups. Which of the following values would indicate good control of Lowella’s diabetes?
6.75 mmol/L
145 mg/dL
None of the choices is correct
9.5 mmol/L

Answer 12

6.75 mmol/L

Question 13

A patient came in complaining of mental confusion and blurring of speech and sight. A Benedict’s test was done on her urine sample. Which of the following options below would be her expected result?
A brick red precipitate will be expected.
A green precipitate will be visible
None of the choices is correct
It will produce a blue color.

Answer 13

A green precipitate will be visible

Question 14

Which of the following are the products of glucose oxidation by glucose oxidase?
1. Gluconic acid
2. Hydrogen peroxide
3. H2O
4. 4-aminoantipyrine
1,2,4
1,3,4
2,3,4
1,2,3

Answer 14

-1,2,3

Question 15

Which enzyme oxidizes the p- amino antipyrine in the GOD-PAP method to give a colored compound?
Glucose oxidase
Glucose dehydrogenase
Hexokinase
Peroxidase
-

Answer 15

Peroxidase
-

Question 16

What makes the reaction in the hexokinase method specific for glucose determination?
GIDH
ADP
G6PD
NADPH

Answer 16

G6PD

Question 17

A patient came in the clinic today and presents symptoms of hyperglycemia. What will be the action of insulin during this state?
Increasing the transport entry of glucose in muscle and adipose tissue by way of nonspecific receptors.
Increasing glycogenolysis, and promoting lipolysis.
Reducing blood glucose levels by glycogenolysis in the liver and an increase in gluconeogenesis.
Increases intestinal absorption of glucose.

Answer 17

-Increasing the transport entry of glucose in muscle and adipose tissue by way of nonspecific receptors.

Question 18

What glucose test is used by diabetics to monitor their daily glucose levels while undergoing insulin therapy?
Capillary blood glucose
HbAlc
Oral Glucose Tolerance test
Fasting blood glucose

Answer 18

-Capillary blood glucose

Question 19

A doctor wanted to know if a patient is following the dietary plan to control his blood sugar for the past 2 months, what test should the doctor order and why?
The doctor should use HbA1c, since it requires whole blood for its analysis, this it reflects the true plasma values of the patient.
The physician should order a HbA1c, since it tests blood glucose attached to hemoglobin in the RBCs, reflecting the average blood glucose over the past weeks.
He should request a Capillary blood glucose test, since it can monitor the blood sugar levels of the patient for the following weeks.
The doctor should order a 2-hr postprandial test. because it tests the glucose utilization of the after a meal.

Answer 19

-The physician should order a HbA1c, since it tests blood glucose attached to hemoglobin in the RBCs, reflecting the average blood glucose over the past weeks.

Question 20

The colored solution that results from adding the reagent to serum for the glucose oxidase coupled reaction is shown below. Which of the following is responsible in the generation the color reaction?

4-amino antipyrine-dimethylaniline
2.2”-azino-di-3-ethvlbenzothiazoline sulfonic acid - 6-diammonium salt
O-dianisidine
O-toluidine

Answer 20

O-dianisidine