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Biochem Lab > M7 Experiments > Flashcards

M7 Experiments Flashcards

1
Q

Test for Peptide Bonds. A general test for Protein and Polypetides

A

Biuret Test

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2
Q

Test for the presence of @-amino acids in proteins.

A

Ninhydrin Test

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3
Q

Test for the presence of aromatic amino acids

A

Xanthoproteic test

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4
Q

Test for the presence of tryptophan

A

Hopkins-Cole Test

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5
Q

Test for phenolic compounds. also used to detect presence of tyrosin

A

Millon’s Test

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6
Q

Test for the presence of cysteine or cystine

A

Lead Sulfide Test

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7
Q

Reagent of Biuret test

A

NaOh + CuSO4

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8
Q

Reagent of ninhydrin test

A

2,2 dihydroxyindane- 1,3-dione

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9
Q

reagent of xanthoproteic test

A

HNO3 + NaOH

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10
Q

reagent of hopkin’s cole

A

Glyoxylic acid +H2SO4

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11
Q

Reagent of Millon’s test

A

HgNO3 in HNO3

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12
Q

test samples of biuret test

A

gelatin, egg albumin, glycine

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13
Q

test samples of ninhydrin test

A

egg albumin, alanine, gelatin

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14
Q

test samples of xanthoproteic test

A

Egg albumin, tyrosine, alanine, phenylalanine

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15
Q

test samples of hopkin-cole’s test

A

albumin, casein, tryptophan, gelatin

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16
Q

test samples of millon’s test

A

casein, tyrosine, phenol

17
Q

test samples of lead sulfide test

A

albumin, tyrosine, casein

18
Q

reagent of lead-sulfide test

A

NaOH + PbAc

19
Q

Positive result of biuret test

A

Violet-pink color

20
Q

negative result of biuret test

A

blue color

21
Q

positive result of ninhydrin test

A

blue-violet complex

22
Q

negative result of ninhydrin test

A

clear

23
Q

proline on ninhydrin test

A

yellow

24
Q

positive result of xanthoproteic test

A

yellow/oraneg

25
Q

negative result of xanthoproteic test

A

clear

26
Q

positive result of hopkins-cole test

A

violet ring at junction

27
Q

negative result of hopkins cole test

A

clear

28
Q

positive result of millons test

A

red ppte

29
Q

negative result of millons test

A

clear

30
Q

positive result of lead sulfide test

A

black/brown ppte

31
Q

negative result of lead sulfide

A

clear

32
Q

positive test samples of biuret test

A

egg albumin, gelatin

33
Q

positive samples of ninhydrin test

A

egg albumin, gelatin, alanine

34
Q

positive test samples of millons test

A

tyrosine, casein

35
Q

positive test samples of lead sulfide

A

albumin

Biochem Lab (4 decks)

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