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Biochem Lab > M8 to M10 Experiments > Flashcards

M8 to M10 Experiments Flashcards

1
Q

Reaction with Heat test samples

A

albumin, casein, gelatin

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2
Q

Result of Albumin in when heated

A

cloudy, white ppte coagulate

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3
Q

result of casein when heated

A

clear

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4
Q

result of gelatin when heated

A

clear no reaction w/n hot but coagulates when cold

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5
Q

factors that cause denaturation

A

heavy metals, alkaloidal reagents, concentrated acids, alkali, organic solvent

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6
Q

Reaction with Heavy Metals

A

AgNO3, HgCL2, PbAc2

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7
Q

reaction of AgNO3 with egg albumin

A

white ppt

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8
Q

reaction of HgCL2 with egg albumin

A

white ppte

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9
Q

reaction of PbAc2 with egg albumin

A

black ppte

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10
Q

Alkaloidal reagents

A

Picric Acid, Tannic Acid, TCA

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11
Q

reaction of picric acid with albumin

A

yellow ppte

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12
Q

reaction of tannic acid with albumin

A

white/brown ppte

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13
Q

reaction of TCA with albumin

A

white ppte

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14
Q

concentrated acids

A

HNO3, HCl

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15
Q

Alcohol percentages

A

95%, 70%, 40%

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16
Q

reaction of 95% ethanol

A

mostly cloudy/ max ppte

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17
Q

reaction of 70% ethanol

A

moderately cloudy

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18
Q

reaction of 40% ethanol

A

least cloudy/ min ppte

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19
Q

positive result of Activity of Amylase

A

blue

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20
Q

negative result of activity of amylase

A

yellow/brown stays the same

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21
Q

Saliva + ? + I2 soln

A

starch

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22
Q

test to detect the presence of reducing sugars

A

Fehlings A and B

23
Q

positive result of Fehlings A and B

A

brick red ppte

24
Q

Saliva + Starch + ? + Fehlings A and B

A

Iodine solution

25
Q

testing for aldehydes and reducing sugars

A

Fehlings Test

26
Q

A soln containing copper (II) sulfate

A

Fehlings A

27
Q

a clear liquid consisting of potassium sodium tartrate + sodium hydroxide

A

Fehlings B

28
Q

Fehlings Test Reagent

A

Fehlings A and B

29
Q

positive result of fehlings test

A

brown

30
Q

negative result of fehlings

A

no reaction

31
Q

test samples of fehlings test

A

glucose, sucrose

32
Q

positive test samples of fehlings test

A

glucose and sucrose in citric acid

33
Q

to detect an aldehyde group or sometimes alpha-hydroxy ketone

A

tollen’s test

34
Q

tollen’s reagent

A

Agno3 + NH4OH + NaOH

35
Q

positive result of tollen’s test

A

silver mirror

36
Q

test sample of tollen’s test

A

dextrose

37
Q

is dextrose positive result for tollen’s test

A

yes

38
Q

tests to detect phosphates

A

Dry heating test, Independent Radicals Test, Magnesia Mixture Test, confirmatory test of phosphate

39
Q

positive result of dry heating test

A

voluminous mass

40
Q

positive result of independent radicals test

A

yellow ppte

41
Q

positive result of magnesia mixture test

A

white ppte

42
Q

special test for purines

A

Uric acid test

43
Q

extracted from yeast

A

test to detect RNA

44
Q

positive result of yeast extract + NaOH + CuSO4

A

violet soln

45
Q

result of hydrolyzate + NaOH + AgNO3

A

light brown ppte

46
Q

result of hydrolyzate + bial’s orcinol

A

blue/green soln

47
Q

test to detect chemical presence of pentoses

A

bials orcinol test

48
Q

bials orcinol reagent

A

orcinol + hydrochloric acid + ferric chloride

49
Q

positive result of bials orcinol

A

blue/green soln

50
Q

negative result of bials orcinol test

A

muddy brown soln

51
Q

test samples of bials orcinol

A

ribose and gluce

52
Q

is ribose positive in bials orcinol

A

yes

53
Q

is glucose positive in bials orcinol

A

no it is negative

Biochem Lab (4 decks)

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